CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2023, Vol. 45 ›› Issue (1): 164-174.doi: 10.19802/j.issn.1007-9084.2021322

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Effects of chitooligosaccharide soaking on germination and physiological metabolism of peanut seeds

Xin-long SHI1,2(), Yue-qin YANG1(), Suo-yi HAN2, Xian XUE1, Jun-jia GUO2, Ya-qi WANG2, Xin-you ZHANG1,2()   

  1. 1.Agriculture College / Tree Peony College of Henan University of Science and Technology, Luoyang 471000, China
    2.Institute of Crop Molecular Breeding of Henan Academy of Agricultural Sciences, Zhengzhou 450003, China
  • Received:2021-12-22 Online:2023-02-25 Published:2023-03-03
  • Contact: Yue-qin YANG,Xin-you ZHANG E-mail:975184724@qq.com;yyqyxf@126.com;haasxinyou@163.com


To investigate the effects of chitooligosaccharide (COS) soaking on germination and physiological metabolism of peanut seeds under low temperature, seed germination and related physiological indexes of peanut were observed following immersion in chitooligosaccharide solution. Results showed that 250 mg/L COS significantly increased peanut germination at low temperature. Compared with no soaking control, both germination rate and relative germination rate increased by 44.45 percentage points, germination index and seed vigor index increased by 5.59 and 24.83, respectively. COS soaking also significantly increased gibberellin content and decreased abscisic acid content of peanut seeds during germination. The content of gibberellin in germinating seeds at 0, 12, 24, 36 and 48 h were significantly increased by 7.94%, 3.84%, 8.52%, 12.40% and 19.20%, and abscisic acid content was significantly decreased by 38.41%, 36.99%, 38.03%, 41.20%, 40.12%. Meanwhile, COS soaking increased the activities of lipase, protease, α-amylase, superoxide dismutase, peroxidase and catalase, and accumulated the quantities of adenosine triphosphate, soluble sugar, soluble protein and proline, while decreased the contents of malondialdehyde and hydrogen peroxide. Studies revealed that COS could regulate hormone level of peanut seeds and improve the activities of hydrolase at the early germination stage under low temperature environment, promote the decomposition of storage substances, and also enhance the activity of antioxidant enzymes, increase the content of osmotic regulatory substances, slow down lipid membrane peroxidation, which promote seed germination.

Key words: peanut, chitooligosaccharide, seed germination, low temperature, hormones, antioxidant enzyme promoting system

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