Effects of chitooligosaccharide soaking on germination and physiological metabolism of peanut seeds

doi:10.19802/j.issn.1007-9084.2021322

Abstract

Abstract:

To investigate the effects of chitooligosaccharide （COS） soaking on germination and physiological metabolism of peanut seeds under low temperature, seed germination and related physiological indexes of peanut were observed following immersion in chitooligosaccharide solution. Results showed that 250 mg/L COS significantly increased peanut germination at low temperature. Compared with no soaking control, both germination rate and relative germination rate increased by 44.45 percentage points, germination index and seed vigor index increased by 5.59 and 24.83, respectively. COS soaking also significantly increased gibberellin content and decreased abscisic acid content of peanut seeds during germination. The content of gibberellin in germinating seeds at 0, 12, 24, 36 and 48 h were significantly increased by 7.94%, 3.84%, 8.52%, 12.40% and 19.20%, and abscisic acid content was significantly decreased by 38.41%, 36.99%, 38.03%, 41.20%, 40.12%. Meanwhile, COS soaking increased the activities of lipase, protease, α-amylase, superoxide dismutase, peroxidase and catalase, and accumulated the quantities of adenosine triphosphate, soluble sugar, soluble protein and proline, while decreased the contents of malondialdehyde and hydrogen peroxide. Studies revealed that COS could regulate hormone level of peanut seeds and improve the activities of hydrolase at the early germination stage under low temperature environment, promote the decomposition of storage substances, and also enhance the activity of antioxidant enzymes, increase the content of osmotic regulatory substances, slow down lipid membrane peroxidation, which promote seed germination.

Key words: peanut, chitooligosaccharide, seed germination, low temperature, hormones, antioxidant enzyme promoting system

CLC Number:

S565.2

Xin-long SHI, Yue-qin YANG, Suo-yi HAN, Xian XUE, Jun-jia GUO, Ya-qi WANG, Xin-you ZHANG. Effects of chitooligosaccharide soaking on germination and physiological metabolism of peanut seeds[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2023, 45(1): 164-174.

Figures/Tables 8

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处理缩写 Abbreviation	处理名称 Treatment
CK0	常温（25℃）对照处理 CK0
LT0/CK	低温（15℃）对照处理CK Low temperature （LT， CK）
LT1	低温处理+ 50 mg/L COS （LT+ 50 mg/L COS）
LT2	低温处理+ 100 mg/L COS （LT+ 100 mg/L COS）
LT3	低温处理+ 150 mg/L COS （LT+ 150 mg/L COS）
LT4	低温处理+ 200 mg/L COS （LT+ 200 mg/L COS）
LT5	低温处理+ 250 mg/L COS （LT+ 250 mg/L COS）
LT6	低温处理+ 300 mg/L COS （LT+ 300 mg/L COS）
LT7	低温处理+ 350 mg/L COS （LT+ 350 mg/L COS）

处理Treat.	露白率 Percentage of seeds with radicals breaking through testa /%
处理Treat.	1 d	2 d	3 d	4 d	5 d	6 d	7 d
CK0	41.11±6.94a	91.11±3.85a	97.78±1.92a	100.00±0.00a	100.00±0.00a	100.00±0.00a	100.00±0.00a
LT0	0.00±0.00d	10.00±3.33d	30.00±6.67f	72.22±3.85d	90.00±5.77c	91.11±3.85b	91.11±3.84b
LT1	0.00±0.00d	13.33±3.34d	46.67±3.34e	73.33±5.77d	91.11±1.92c	91.11±1.92b	91.11±1.92b
LT2	0.00±0.00d	22.22±1.92c	57.78±5.09de	88.89±5.09bc	94.45±3.85ab	95.56±1.93ab	95.56±1.93ab
LT3	3.33±3.34cd	35.56±1.93b	82.22±3.85b	88.89±7.70bc	92.22±5.09c	94.45±6.94ab	94.45±6.94ab
LT4	3.33±0.00cd	31.11±10.18b	68.89±10.71cd	90.00±0.00abc	91.11±1.92c	94.44±1.93ab	94.44±1.93ab
LT5	5.56±1.93c	33.34±5.77b	86.67±8.82ab	95.56±5.09ab	96.67±3.34ab	96.67±3.34ab	96.67±3.34ab
LT6	7.78±1.92bc	37.78±6.94b	83.33±8.82b	95.56±1.93ab	96.67±0.00ab	96.67±0.00ab	96.67±0.00ab
LT7	12.22±1.92b	35.56±1.93b	75.55±3.85bc	84.44±10.18c	92.22±5.09c	92.22±5.09b	92.22±5.09b

处理 Treat.	发芽率 /% Germination rate	相对发芽率 /% Relative germination rate	发芽指数 Germination index	种子活力指数 Seed vigor index	根长胁迫指数 Root length stress index
CK0	100.00±0.00a	100.00±0.00a	27.93±1.57a	225.60±3.78a	100.00±4.95a
CK/LT0	3.33±3.33f	3.33±3.33f	0.20±0.23d	0.32±0.41e	12.64±11.21e
LT1	4.44±1.92f	4.44±1.92f	0.26±0.20d	0.29±0.21e	14.25±0.72e
LT2	13.33±3.33e	13.33±3.33e	1.09±0.07d	1.65±0.03e	18.70±1.30e
LT3	20.00±5.77e	20.00±5.77e	1.85±0.30cd	3.34±0.45e	22.41±1.17e
LT4	34.44±5.09d	34.44±5.09d	3.29±0.38c	8.88±2.74d	32.83±6.93d
LT5/COS	47.78±5.09b	47.78±5.09b	5.79±0.61b	25.15±0.94b	54.04±5.01b
LT6	44.44±5.09bc	44.44±5.09bc	5.37±1.28b	25.20±1.64b	46.21±5.05bc
LT7	37.78±6.94cd	37.78±6.94cd	5.66±2.12b	19.22±6.59c	42.63±3.98c

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