Localization and function of effector P2 of Puccinia helianthi Schw

doi:10.19802/j.issn.1007-9084.2022012

Abstract

Abstract:

Sunflower rust, caused by Puccinia helianthi, often threatens sunflower production. Effectors are proteins secreted during pathogen infection, which promote pathogen invasion or trigger host immunity. In order to clarify the role of P2 effector in rust infection process, P2 gene was analyzed by bioinformatics. cDNA of P. helianthi urediospores was used as template to clone P2 gene. Its expression characteristics during the infection were analyzed by qRT-PCR. Then function of P2 was verified in Nicotiana benthamiana by Agrobacterium transient expression system, and subcellular localization of P2 was performed by transient expression in N.benthamiana. Results showed that P2 encoded 58 amino acids （aa） with 24 aa signal peptide at N-terminal, without nuclear localization signal and transmembrane domain. P2 expression was up-regulated during early stage of P. helianthi infection. Transient expression of P2 in N.benthamiana inhibited BAX-induced cell death. Subcellular localization showed that P2 was localized in cell membrane and nucleus.

Key words: sunflower rust, effector, qRT-PCR, programmed cell death (PCD), subcellular localization

CLC Number:

S435.655

Jia-peng JIN, Xiao-yu LIAN, Yan LU, Lan JING. Localization and function of effector P2 of Puccinia helianthi Schw[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2023, 45(1): 124-130.

Figures/Tables 5

Table 1

Fig. 1

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Fig. 4

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引物 Primer	序列 Sequence （5'-3'）
UBC-F	GTGGTGACCAAAGACTGAGACA
UBC-R	CGACAGTCAGCCAACTACC
qPCR-P2-F	TGCCACGTTGAGAATGACTC
qPCR-P2-R	TGCTTCCCAGCATAACCTTT
P2-F	CGGGGTACCATGATCATTGGCTTTGATCTGTTGG
P2-R	GCGTCGACTTAAAATGCTTCCCAGCATAACCTT
part-CAM-eGFP-P2-F	TTGGAGAGGACACGCTCGAGATGATCATTGGCTTTGATCT
part-CAM-eGFP-P2-R	CCCTTGCTCACCATGAATTCAAATGCTTCCCAGCATAAC

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