Analysis of phosphorus efficiency and screening of P-efficient germplasm on natural population of oilseed rape (Brassica napus) at seedling stage

doi:10.19802/j.issn.1007-9084.2022087

Abstract

Abstract:

Screening of phosphorus (P)-efficient germplasm of oilseed rape (Brassica napus L.) will provide elite materials for P heredity and P-efficient breeding. In this study, 400 varieties of natural population of oilseed rape (B. napus) was used to evaluate P efficiency at seedling stage under 2 experiments by hydroponic culture, named EXP. Ⅰ and EXP. Ⅱ, respectively. Under normal P and low P conditions, RDW (root dry weight), SDW (shoot dry weight), RSR (root : shoot ratio), PRL (primary root length) and PEC (phosphorus efficiency coefficient) of the natural population showed normal distribution, indicating that there existed abundant genetic variation in the natural population. P efficiency of the natural population of B. napus at the seedling stage was determined by the parameter of SDW_LP and PEC. Among them, 17 P-efficient and 13 P-inefficient candidate varieties were obtained in EXP. Ⅰ, while 17 P-efficient and 18 P-inefficient candidate varieties were obtained in EXP. Ⅱ. Based on the 2 experiments, totally 32 P-efficient and 31 P-inefficient candidate varieties were identified for further work.

Key words: Brassica napus, phosphorus, phosphorus efficiency, germplasm screening

CLC Number:

S565.4

Yan-ling CHEN, Ning WANG, Lei SHI. Analysis of phosphorus efficiency and screening of P-efficient germplasm on natural population of oilseed rape (Brassica napus) at seedling stage[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2023, 45(1): 56-62.

Figures/Tables 6

Fig. 1

Table 1

Table 2

Table 3

Table 4

Table 5

References 23

1	中华人民共和国国家统计局. 中国统计年鉴［M］. 北京：中国统计出版社，2021.
2	Cordell D， Drangert J O， White S. The story of phosphorus： global food security and food for thought［J］. Glob Environ Change， 2009， 19： 292-305. DOI： 10.1016/j.gloenvcha.2008.10.009 . doi: 10.1016/j.gloenvcha.2008.10.009
3	Roland W S， Andrea E U， Marjatta E， et al. Sustainable use of phosphorus： A finite resource［J］. Sci Total Environ， 2013： 799- 803. DOI： 10.1016/j.scitotenv.2013.05.043 . doi: 10.1016/j.scitotenv.2013.05.043
4	Hinsinger P. Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes： a review［J］. Plant & Soil， 2001， 237（2）：173-195. DOI： 10.1023/A：1013351617532 . doi: 10.1023/A：1013351617532
5	Richardson A E， Simpson R J. Soil microorganisms mediating phosphorus availability update on microbial phosphorus［J］. Plant Physiology， 2011， 156（3）： 989-996.DOI： 10.1104/pp.111.175448 . doi: 10.1104/pp.111.175448
6	Gregory P J， George T S. Feeding nine billion： the challenge to sustainable crop production［J］. J Exp Bot， 2011， 62（15）： 5233-5239. DOI： 10.1093/jxb/err232 . doi: 10.1093/jxb/err232
7	López-Arredondo D L， Leyva-González M A， González-Morales S I， et al. Phosphate Nutrition： Improving low-phosphate tolerance in crops［J］. Annu Rev Plant Biol， 2014， 65： 95-123. DOI： 10.1146/annurev-arplant-050213-035949 . doi: 10.1146/annurev-arplant-050213-035949
8	Narang R A， Bruene A， Altmann T. Analysis of phosphate acquisition efficiency in different Arabidopsis accessions［J］. Plant Physiol， 2000， 124： 1786-1799. DOI： 10.1104/pp.124.4.1786 . doi: 10.1104/pp.124.4.1786
9	Wang X R， Shen J B， Liao H. Acquisition or utilization， which is more critical for enhancing phosphorus efficiency in modern crops？［J］. Plant Sci， 2010， 179（4）： 302-306.93. DOI： 10.1016/j.plantsci.2010.06.007 . doi: 10.1016/j.plantsci.2010.06.007
10	Ozturk L， Eker S， Torun B， et al. Variation in phosphorus efficiency among 73 bread and durum wheat genotypes grown in aphosphorus-deficient calcareous soil［J］. Plant Soil， 2005， 269： 69-80. DOI： 10.1007/s11104-004-0469-z . doi: 10.1007/s11104-004-0469-z
11	刘慧，刘景福，刘武定. 不同磷营养油菜品种根系形态及生理特性差异的研究［J］. 植物营养与肥料学报，1999，5（1）：40-45. DOI： 10.3321/j.issn：1008-505X.1999.01.007 . doi: 10.3321/j.issn：1008-505X.1999.01.007
12	Duan H Y， Shi L， Ye X S， et al. Identification of phosphorous efficient germplasm in oilseed rape［J］. J of Plant Nutr， 32： 1148-1163， 2009. DOI： 10.1080/01904160902943171 . doi: 10.1080/01904160902943171
13	Yang M， Ding G D， Shi L， et al. Quantitative trait loci for root morphology in response to low phosphorus stress in Brassica napus ［J］. Theor Appl Genet， 2010， 121： 181-193. DOI： 10.1007/s00122-010-1301-1 . doi: 10.1007/s00122-010-1301-1
14	梁宏玲，石磊，徐芳森，等. 甘蓝型油菜不同磷效率基因型对土壤难溶性磷吸收利用的差异［J］. 中国油料作物学报，2007，29（3）：297-301.
15	Zhang H W， Huang Y， Ye X S， et al. Genotypic differences in phosphorus acquisition and the rhizosphere properties of Brassica napus in response to low phosphorus stress［J］. Plant Soil， 2009， 32： 91-102. DOI： 10.1007/s11104-008-9873-0 . doi: 10.1007/s11104-008-9873-0
16	Ahmad Z， Gill M A， Qureshi R H. Genotypic variations of phosphorus utilization efficiency of crops［J］. J Plant Nutr， 2001， 24（8）：1149-1171. DOI： 10.1081/PLN-100106973 . doi: 10.1081/PLN-100106973
17	Gunes A， Inal A， Alpaslan M， et al. Genotypic variation in phosphorus efficiency between wheat cultivars grown under greenhouse and field conditions［J］. Soil Sci Plant Nutr， 2010， 52（4）： 470-478. DOI： 10.1111/j.1747-0765.2006.00068.x . doi: 10.1111/j.1747-0765.2006.00068.x
18	Hayes J E， Zhu Y G， Mimura T， et al. An assessment of the usefulness of solution culture in screening for phosphorus efficiency in wheat［J］. Plant Soil， 2004， 261（1）： 91-97. DOI： 10.1023/B：PLSO.0000035561.00460.8b . doi: 10.1023/B：PLSO.0000035561.00460.8b
19	Clark R B， Duncan R R. Improvement of plant mineral nutrition through breeding［J］. Field Crops Res， 1991， 27： 219-240. DOI： 10.1016/0378-4290（91）90063-2 . doi: 10.1016/0378-4290（91）90063-2
20	王运华，刘武定，程建尧. 油菜磷素营养与磷肥有效施用技术的研究：Ⅰ. 油菜磷素营养的规律性［J］. 湖北农业科学，1973，7 ：14-22.
21	张海伟，黄宇，叶祥盛，等. 甘蓝型油菜重组自交系苗期磷效率的评价［J］. 作物学报，2008，34（12）：2152-2159. DOI： 10.3724/SP.J.1006.2008.02152 . doi: 10.3724/SP.J.1006.2008.02152
22	石桃雄，王少思，石磊，等. 不同氮、磷水平对“双高”油菜品种宁油7号和“双低”油菜品种Tapidor生长和品质的影响［J］. 植物营养与肥料学报，2010，16（4）：959-964. DOI： 10.11674/zwyf.2010.0427 . doi: 10.11674/zwyf.2010.0427
23	Richardson A E， Barea J M， McNeill A M， et al. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms［J］. Plant Soil， 2009， 321（1-2）： 305-339. DOI： 10.1007/s11104-009-9895-2 . doi: 10.1007/s11104-009-9895-2

	地上部干重SDW	根系干重RDW	根冠比 RSR	主根长PRL	磷效率系数PEC
地上部干重SDW		0.768**	-0.365**	0.066	-0.772**
根系干重RDW	0.756**		0.260**	0.043	-0.561**
根冠比RSR	-0.393**	0.285**		0.049	0.420**
主根长PRL	0.136**	0.304**	0.227**		-0.160**
磷效率系数PEC	0.333**	0.170**	-0.232**	0.173**

	地上部干重SDW	根系干重RDW	根冠比 RSR	主根长PRL	磷效率系数PEC
地上部干重SDW		0.705**	-0.447**	0.097	-0.663**
根系干重RDW	0.739**		0.208**	0.187**	-0.375**
根冠比RSR	-0.178**	0.130**		0.072	0.563**
主根长PRL	0.169**	0.284**	0.043		-0.111
磷效率系数PEC	0.473**	0.299**	-0.27**	0.048

		地上部干重SDW	根系干重RDW	根冠比 RSR	主根长PRL	磷效率系数PEC
低磷 LP	EXP. Ⅰ vs Ⅱ	0.314^**	0.369^**	0.398^**	0.340^**	0.020
正常磷 NP	EXP. Ⅰ vs Ⅱ	0.112^*	0.192**	0.027	0.183^**	0.020

磷高效候选品种 Candidate P-efficient cultivars			磷低效候选品种 Candidate P-inefficient cultivars
品种编号	地上部干重	磷效率系数	品种编号	地上部干重	磷效率系数
No.	SDW_LP /g	PEC	No.	SDW_LP /g	PEC
7	0.1558	0.6993	66	0.0717	0.1944
25	0.1895	0.9303	81	0.0839	0.1367
35	0.2037	0.9417	82	0.0923	0.2057
42	0.2040	0.7320	116	0.0923	0.1572
45	0.1606	0.6305	151	0.0625	0.1003
49	0.1888	0.8281	183	0.1010	0.2143
56	0.1563	0.5934	242	0.0997	0.1571
70	0.1681	0.6360	267	0.0961	0.2258
92	0.1800	0.7692	349	0.0834	0.1591
101	0.2039	0.9336	365	0.0970	0.2183
102	0.1831	0.7206	386	0.0878	0.0974
130	0.1799	0.8915	396	0.0837	0.1717
142	0.2039	0.8640	397	0.0796	0.1946
208	0.1643	0.9008
306	0.1729	0.8187
333	0.1712	0.7392
360	0.2092	0.6511

磷高效候选品种			磷低效候选品种
Candidate P-efficient cultivars			Candidate P-inefficient cultivars
品种编号	地上部干重	磷效率系数	品种编号	地上部干重	磷效率系数
No.	SDW_LP /g	PEC	No.	SDW_LP /g	PEC
49	0.1340	0.6256	87	0.0716	0.1371
65	0.1419	0.6176	88	0.0593	0.1412
66	0.1361	0.9873	91	0.0495	0.1398
102	0.1667	0.5310	131	0.0675	0.1314
169	0.1334	0.6712	136	0.0531	0.1093
174	0.1452	0.5830	187	0.0471	0.0942
175	0.1463	0.7116	226	0.0574	0.0795
194	0.1573	0.5574	227	0.0753	0.1345
195	0.1352	0.6643	231	0.0614	0.0577
196	0.1483	0.7153	232	0.0680	0.1150
198	0.1681	0.6544	233	0.0718	0.1379
199	0.1799	0.5299	240	0.0737	0.1280
200	0.1995	0.5639	250	0.0722	0.1385
276	0.1656	0.4779	252	0.0667	0.1125
312	0.1543	0.5731	350	0.0741	0.1468
317	0.1635	0.4856	368	0.0433	0.1202
389	0.1313	0.4801	374	0.0554	0.1429
			381	0.0722	0.1367