Comprehensive evaluation of Jihua peanut varieties with high oleic acid based on principal component and cluster analysis

doi:10.19802/j.issn.1007-9084.2022191

Abstract

Abstract:

To guide classification of peanut germplasm resources, principal component and cluster analysis were applied on 11 Jihua varieties with high oleic acid by using 3 years phenotype and re-sequencing data. Results showed that oil content variation coefficient among 15 traits was the smallest at 1.91%. The variation of yield characteristics was greater than quality except linoleic acid content. The positive correlation coefficient between hundred-pod weight and hundred-seed weight was extremely significant, and oleic acid content had a significant negative correlation with linoleic acid content. The cumulative contribution rate of the first two principal components was 78.99%. Cultivars were classified using phenotype traits, and the results were partially consistent with pedigree relationships. After data control, 320 000 high-quality SNP sites with uniform density distribution were obtained. 4 categories were from 11 varieties via genotype data. Meanwhile, varieties derived from the same combination were divided into one category, thus the results are consistent with variety pedigree relationship. Therefore, genotype data is more accurately on reflecting genetic basis of the varieties, and could provide reference for germplasm resources classification and utilization.

Key words: peanut, high oleic acid, principal component analysis, cluster analysis, phenotype, SNP site

CLC Number:

S565.2

Min-jie GUO, Li DENG, Yu-rong Li, Jin WANG, Li REN. Comprehensive evaluation of Jihua peanut varieties with high oleic acid based on principal component and cluster analysis[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(6): 1210-1217.

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品种 Cultivar	系谱 Pedigree
冀花915 Jihua 915	开农65×P08-1 Kainong 65×P08-1
冀914 Ji 914	开农65×P08-1 Kainong 65×P08-1
冀花11号 Jihua 11	冀花5号×开选016 Jihua 5×Kaixuan 016
冀花18号 Jihua 18	冀花5号×开选016 Jihua 5×Kaixuan 016
冀花13号 Jihua 13	冀花6号×开选016 Jihua 6×Kaixuan 016
冀花16号 Jihua 16	冀花6号×开选016 Jihua 6×Kaixuan 016
冀花19号 Jihua 19	冀花6号×开选016 Jihua 6×Kaixuan 016
冀花21号 Jihua 21	冀花6号×开选016 Jihua 6×Kaixuan 016
冀花26号 Jihua 26	冀花6号×开选016 Jihua 6×Kaixuan 016
冀花28号 Jihua 28	冀花13号×漯花9号 Jihua 13×Luohua 9
冀花572 Jihua 572	秋乐花177×冀花16号 Qiulehua 177×Jihua 16

性状 Trait	2019年均值 Mean （2019）	2020年均值 Mean （2020）	2021年均值 Mean （2021）	平均值 Mean value	标准差 SD	变异系数 CV /%
百果重Hundred-pod weight /g （Y₁）	185.40	188.80	203.54	192.58	25.33	13.15
市斤果数 No. of pod /500g （Y₂）	369.27	337.39	335.12	347.26	53.11	15.29
饱果率Matural pod percentage /% （Y₃）	80.60	70.19	64.88	71.89	5.63	7.83
出米率Shelling percentage /% （Y₄）	70.14	67.08	63.27	66.83	2.68	4.01
半斤仁数No. of seed /250g （Y₅）	427.70	439.68	446.68	438.02	47.99	10.96
百仁重Hundred-seed weight /g （Y₆）	74.67	71.04	75.00	73.57	6.92	9.41
荚果长Pod length /mm （Y₇）	35.87	37.84	35.73	36.48	3.31	9.07
荚果宽Pod width /mm （Y₈）	15.85	15.43	15.94	15.74	1.08	6.85
籽仁长Seed length /mm （Y₉）	17.74	17.83	17.35	17.64	1.14	6.47
籽仁宽Seed width /mm （Y₁₀）	9.45	8.80	9.44	9.23	0.27	2.93
蛋白质含量Protein content /% （Q₁）	24.95	26.72	22.49	24.72	0.60	2.41
脂肪含量Fat content /% （Q₂）	49.54	47.15	52.28	49.66	0.95	1.91
油酸含量Oleic acid content /% （Q₃）	76.38	69.49	80.12	75.33	2.15	2.86
亚油酸含量 Linoleic acid content /% （Q₄）	5.09	12.47	2.06	6.54	2.11	32.21
棕榈酸含量Palmitic acid content /% （Q₅）	7.34	7.06	6.75	7.05	0.19	2.68

主成分 Component	特征值 Eigenvalue	贡献率/% Contribution rate	累计贡献率 /% Cumulative contribution rate
1st	9.4941	63.29	63.29
2nd	2.3549	15.70	78.99
3rd	1.6801	11.20	90.19
4th	0.7994	5.33	95.52
5th	0.2816	1.88	97.40
6th	0.1832	1.22	98.62
7th	0.1671	1.11	99.74
8th	0.0266	0.18	99.91
9th	0.0094	0.06	99.98
10th	0.0035	0.02	100.00

性状 Trait	主成分 Component
性状 Trait	1st	2nd
百果重Hundred-pod weight /g （Y₁）	0.31	-0.30
市斤果数 No. of pod /500g （Y₂）	-0.15	0.15
饱果率Matural pod percentage /% （Y₃）	-0.08	0.16
出米率Shelling percentage /% （Y₄）	-0.03	0.16
半斤仁数No. of seed /250g （Y₅）	0.18	0.38
百仁重Hundred-seed weight /g （Y₆）	-0.11	0.05
荚果长Pod length /mm （Y₇）	0.09	0.12
荚果宽Pod width /mm （Y₈）	0.10	-0.29
籽仁长Seed length /mm （Y₉）	0.33	-0.10
籽仁宽Seed width /mm （Y₁₀）	0.17	0.44
蛋白质含量Protein content /% （Q₁）	-0.56	0.38
脂肪含量Fat content /% （Q₂）	0.00	-0.23
油酸含量Oleic acid content /% （Q₃）	0.00	0.05
亚油酸含量 Linoleic acid content /% （Q₄）	0.00	0.07
棕榈酸含量Palmitic acid content /% （Q₅）	0.60	0.43

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