
Transcriptomic analysis on the flower color changes of white-petaled BnaC3.CCD4-transgenic rapeseed (Brassica napus) linesduring the daytime
Jin-ting WANG, Xue-yu SUN, Wen-jing HU, Qian YANG, Chao LIU
CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2025, Vol. 47 ›› Issue (1) : 45-59.
Transcriptomic analysis on the flower color changes of white-petaled BnaC3.CCD4-transgenic rapeseed (Brassica napus) linesduring the daytime
Our previous study revealed that BnaC3.CCD4 encodes carotenoid cleavage dioxygenas whose mutations altered flower color from white to yellow in rapeseed. Interestingly, we observed that the white flowers of functional BnaC3.CCD4 transgenic lines showed pale yellow in the morning and still became pure white in the afternoon during the daytime. In order to explore whether environmental signals such as light and temperature have a regulatory effect on carotenoid biosynthesis and metabolism, RNA-seq was performed for transcriptomic analysis on them at two different timepoints in the present research. The analysis results of gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) revealed that a number of differential expression genes were significantly enriched in pathways related to light signal, circadian rhythm, stimulus response and carotenoid biosynthesis et al. Through further analyzing the top 50 differentially expressed genes, it was revealed that the light signal might be the key factor for the flower color change of BnaC3.CCD4 transgenic lines. Therefore, it was inferred that the light signal factors potentially regulate the expressions of the genes in the carotenoid biosynthesis pathway by binding to their photoresponsive elements to control the carotenoid contents in the petals and then cause their color change.
Brassica napus / flower color / carotenoid cleavage dioxygenas / light signal / circadian rhythm {{custom_keyword}} /
Fig. 1 Flower colors and their values of BnaC3.CCD4 transgenic plants at different time points图1 不同时间点BnaC3.CCD4 转基因植株花色及其色度值 |
Fig. 15 qRT-PCR validation for the transcriptome data in BnaC3.CCD4-transgenic lines图15 BnaC3.CCD4转基因株系转录组数据qRT-PCR验证 |
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