PDF(1321 KB)
Genetic diversity of Brassica juncea from Tibet Autonomous Region of China
SONG Wei-lin，XU Kun，LI feng，CHEN Bi-yun, CAI Meng-xian，WU Jin-feng，WU Xiao-ming
CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2013, Vol. 35 ›› Issue (2) : 153.
PDF(1321 KB)
PDF(1321 KB)
Genetic diversity of Brassica juncea from Tibet Autonomous Region of China
Genetic diversity of 73 mustard (Brassica juncea L.) accessions from Tibet Autonomous Region of China and 35 from southwest of China，northwest of China and India were analyzed using 8 SRAP (sequence－related amplified polymorphism) primers combinations on 20 phenotypic traits. SRAP results showed that a total of 139 bands were produced with polymorphism rate of 24.5%. Clustering result of SRAP showed that 108 accessions were divided into 4 groups, and the accessions in each group were mainly from the same region. Genetic diversity index of China Tibet accessions was higher than others. Variation degree of 8 important phenotypic traits of Tibet accessions showed the highest variation coefficiency was 41.29% on silique number per plant. Clustering analysis of phenotypic traits showed that 108 materials were clearly divided into different groups based on their original regions except the accessions from Northwest. In conclusion, Tibet mustard had abundant genetic diversity. The order of genetic diversity indexes from top to bottom was as follows: China Tibet, Southwestern China, Northwestern China and India. Mustard genetic diversity was mainly associated with geological and biological conditions.
Tibet of China / Brassica juncea L. / Genetic diversity / SRAP / Phenotypic traits {{custom_keyword}} /
[1] 刘后利. 油菜遗传育种学[M]. 北京: 中国农业大学出版社, 2000.26-31, 75-80, 82-89, 289-316.
[2] 刘淑艳, 刘忠松, 官春云, 等.芥菜型油菜种质资源研究进展植物遗传资源学报[J]. 2007, 8(3): 351-358.
[3] 刘忠松, 官春云, 陈社员, 等. 芥菜型油菜与甘蓝型油菜种间杂种后代的RAPD分析[J]. 中国农业科 学, 2002, 35(8): 1010-1015.
[4] 李汝刚, 朱 莉, 伍宁丰, 等. 我国芥菜型油菜品种遗传多样性初探[J]. 生物技术通报, 1997（5）: 26-31.
[5] 安贤惠, 陈宝元, 傅廷栋, 等. 利用RAPD标记研究中国芥菜型油菜遗传多样性[J]. 华中农业大学学报, 1999, 18(6): 524-527.
[6] 蒲晓斌, 王茂林, 栾 丽, 等. 中国西南地区芥菜型油菜资源遗传多样性分析[J]. 中国农业科学, 2007, 40(8): 1610-1621.
[7] 王建林, 旦 巴, 胡书银, 等. 西藏油菜遗传多样性和系统发生关系的RAPD分析[J]. 西藏科技, 2002, 11: 17-24.
[8] 栾运芳, 胡书银, 王建林, 等. 西藏油菜品种资源特征特性的研究与利用[J]. 西藏科技, 2002（11）: 25-28.
[9] 王建林, 胡书银, 栾运芳, 等. 西藏芥菜型油菜品种资源及其开发利用的研究[J]. 西藏科技，2002, 11:41-43.
[10] 胡书银, 王建林, 旦 巴, 等. 西藏芥菜型油菜资源的地理分布与表型分化研究[J]. 国土与自然资源研究, 2002, 5(4): 315-319.
[11] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP) a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica [J]. Theor Appl Genet, 2001, 103: 455- 461.
[12] 李巧燕, 林瑞庆, 朱兴全, 等. SRAP分子标记及其应用概述[J]. 热带医学杂志, 2006, 6(4): 467-470.
[13] 王从彦. SRAP标记在植物遗传多样性中的应用[J]. 进展生物技术, 2011, 21(5): 87-90.
[14] Polat I, Kacar Y A, Yesiloglu T, et al. Molecular characterization of sour orange (Citrus aurantium) accessions and their relatives using SSR and SRAP markers[J]. Genet Mol Res, 2012, 11(3): 3267-76.
[15] Zhang Q S, Xu B L, Liu L D, et al. Analysis of genetic diversity among Chinese Pleurotus citrinopileatus Singer cultivars using two molecular marker systems (ISSRs and SRAPs) and morphological traits[J]. World J Microbiol Biotechnol,2012,28(5): 2 237-2 248.
[16] 樊洪泓, 李廷春, 邱 婧, 等. 石斛属几种植物遗传关系的SRAP和RAPD比较分析[J]. 2010, 41(4): 627-632.
[17] 文雁成, 王汉中, 沈金雄, 等. 用SRAP标记分析中国甘蓝型油菜品种的遗传多样性和遗传基础[J]. 中国农业科学, 2006, 39(2): 246-256.
[18] 邹小云, 宋来强, 陈伦林, 等. 利用SRAP标记划分甘蓝型油菜杂种优势群[J]. 江西农业学报,2011, 23(4): 1- 4.
[19] 徐爱遐,马朝芝,肖恩时, 等. 中国西部芥菜型油菜遗传多样性研究[J]. 作物学报, 2008, 34(5): 754?763.
[20] 中国农业科学院油料作物研究所.中国油菜品种资源目录(续编一)[M].农业出版社,1990.
[21] 周必文, 陈道炎, 余 琦, 等. 油菜多抗性材料的鉴定与筛选[J]. 中国油料, 1993, 15(1): 14-17.
[22] 王 珍, 方宣钧. 植物DNA分离[J]. 分子植物育种, 2003, 1(2): 281-288.
[23] 许 鲲, 陆光远, 伍晓明, 等. 欧洲野生甘蓝的核质遗传多样性和群体遗传结构分析. 2011, 33(2): 111-117.
[24] 栾运芳, 胡书银, 王建林, 等. 西藏油菜生长发育规律的研究(J). 西藏科技, 2002（7）: 31-33.
[25] 卓 嘎, 旦 巴, 何 燕, 等. 西藏野生油菜种质资源千粒重分析[J]. 种子, 2008, 27(12): 72-76.
[26] 王建林, 次仁央金, 大次卓嘎, 等. 西藏与周边地区芥菜型油菜农艺性状比较研究[J]. 植物遗传资源学报,2011, 12(4): 562-569.
[27] 钱秀珍, 伍晓明, 胡 琼, 等. 油菜种质资源的搜集鉴定保存和利用[J]. 中国油料, 1996, 18(1): 60-63.
[28] 王胜军, 陆作楣, 万建民, 等. 采用表型和分子标记聚类研究杂交籼稻亲本的遗传多样性[J].中国水稻科学, 2006, 20(5): 475-480.
PDF(1321 KB)
/
| 〈 |
|
〉 |
