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中国油料作物学报 ›› 2015, Vol. 37 ›› Issue (2): 129-.doi: 10.7505/j.issn.1007-9084.2015.02.001

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Fatty acid composition of transgenic high oleic acid rapeseed W-4 (Brassica napus L.)

  

  1. 1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China; 2. Nanjing Sub-Center (Oilseed rape), National Oilseed Crops Improvement Center, Nanjing 210014, China; 3. Key Lab of Cotton and Oilseed Rape of Lower Reaches of Yangtze River, Ministry of Agriculture, Nanjing 210014, China
  • Online:2015-04-28 Published:2015-07-08

Abstract:

To investigated fatty acid changes of a down-regulated fad2 gene transgenic rapeseed line W-4, its T5 to T7 generations and wild type Westar (WT) were used to study seeds fatty acid composition. Results showed that the increase of oleic acid and eicosenoic acid contents were highly significant. Oleic acid was 84.62%±1.41% in W-4 seed, 25.91% higher than that of wild type and eicosenoic acid was 18.46% higher too. While linoleic acid and linolenic acid were 3.22%±0.56% and 3.45%±0.51% respectively, which were 80.89% and 47% lower than that of WT respectively, and the decrease were highly significant. Moreover, palmitic acid (-18.10%) and stearic acid (-8.33%) were lower than that of WT, and the difference were highly significant. The results indicated that down-regulation of fad2 gene significantly changed the fatty acid composition. Thus the polyunsaturated fatty acid contents were significantly reduced, and monounsaturated fatty acid contents were increased.

Key words: Transgenic rapeseed, High oleic acid, Fatty acid composition

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