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中国油料作物学报 ›› 2015, Vol. 37 ›› Issue (3): 277-.doi: 10.7505/j.issn.1007-9084.2015.03.004

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QTL mapping of oil content with wild soybean backcross introgressive lines

  

  1. 1. College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2. Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin,150090, China
  • Online:2015-06-28 Published:2015-09-07

Abstract:

 A soybean backcross population was constructed using Suinong 14 (recurrent parent) and wild soybean ZYD00006 (donor parent). 23 QTLs (4 with positive effects, 19 with negative effects) for oil content on 11 linkage groups were detected by One-way ANOVA at 0.01 level. Compared with recurrent parent Suinong14, 13 BC3F3 lines had higher oil contents and 20 BC3F3 lines had lower oil contents. These 33 lines were selected to construct a control (random) population. Based on this control population, 25 QTLs for oil content on 15 linkage groups were found by genetic hitchhiking; among them, 11 QTLs had positive effect and 14 QTLs had negative effect. Total 34 QTLs were found by these 2 methods. Among them, 14 were detected in both methods, and 11 were newly found.

Key words: Wild soybean, Backcross , Introgression lines, Oil content, QTL mapping