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Identification and genetic stability analysis of transgenic soybean with enhanced level of methionine
HAN Qing-mei,SUN Shi, HOU Wen-sheng, BAI Xiao-feng, YU Yang, ZHOU Yan-feng,HAN Tian-fu
CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2015, Vol. 37 ›› Issue (6) : 789.
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PDF(1367 KB)
Identification and genetic stability analysis of transgenic soybean with enhanced level of methionine
Soybean is one of the most important sources of plant protein, but the content of methionine (Met) in soybean protein is inadequate, which limits its value. Recent researches showed that improving plant protein and amino acids composition of plant protein via bio-technology method is a promising strategy. In the previous study, we transformed AtD-CGS, which is a key gene in Met biosynthesis and is expressed specifically in seeds, into soybean. A homozygous AtD-CGS transgenic soybeans line, Zhongzuo CGS-ZG11, has been obtained by self-fertilization and phenotypic identification through many generations. Zhongzuo CGS-ZG11 was crossed with a conventional soybean variety of Shidou 5 which has good comprehensive characteristics, and generated progeny lines, such as Zhongzuo CGS14J890. The PCR and Southern blot confirmed that AtD-CGS gene was inherited from T6 to T8 generations of Zhongzuo CGS-ZG11. The RT-PCR and Western blot showed the expression of AtD-CGS was stable in Zhongzuo CGS-ZG11. The Met content in different generations of Zhongzuo CGS-ZG11 was investigated by High Performance Liquid Chromatography(HPLC). Compared to the wild-type seeds, the content of total Met in the transgenic soybean seeds increased remarkably, and remained consistently high through generations. The high methionine content in Zhongzuo CGS14J890 further proved that AtD-CGS could function consistently among different genetic background and generations.
Soybean / AtD-CGS gene / Methionine / Genetic stability / hybridization transfer {{custom_keyword}} /
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