Identification of SSR makers associated with bacterial wilt resistance of peanut through correlation analysis

doi:10.7505/j.issn.1007-9084.2015.06.010

Abstract

Abstract:

Bacterial wilt caused by Ralstonia solanacearum is one of the important diseases limiting the production of peanut (Arachis hypogaea L.) in China. In the present study, 72 polymorphic SSR markers were used to detect the genetic diversity of 57 peanut cultivars with various resistance to bacterial wilt (BW). The results showed that the coefficients of genetic similarity between two genotypes among the involved 57 cultivars varied from 0.4978 to 0.9607 with the average of 0.7038. According to UPGMA cluster analysis, the involved 57 cultivars were clustered into two groups when the threshold valve of coefficients of genetic similarity was 0.69. Group I contained 30 accessions including 23 hypogaea genotypes and 7 vulgaris genotypes. 29 of the 30 accessions in group I were susceptible to BW. Group II composed of 27 vulgaris genotypes, among which, 26 were resistant to BW and one was susceptible. The clustering result of the involved 57 cultivars by SSR data was consistent with the botanical type and BW resistance of the cultivars. The genetic diversity among cultivars with BW resistance was slightly greater than that of the susceptible cultivars involved in the present study. Combining the data from bacterial wilt evaluation of the genotypes in two years, a total of 10 SSR loci related to BW resistance were detected through correlation analysis and multiple linear regression analysis, among which, 3 loci including 1B9-3, IPAHM288-1 and ARS590-1 were highly significantly associated with BW resistance. These 3 highly significantly associated with BW resistance makers were used to detect the RIL population from crossing of parents with BW resistance and susceptible respectively, loci 1B9-3 and ARS590-1 were detected in 62.85% and 40.00% of the BW resistant lines.

Key words: Peanut cultivars, Bacterial wilt resistance, Genetic diversity, SSR

XU Zhi-jun, REN Xiao-ping, HUANG Li, CHEN Yu-ning, ZHOU Xiao-jing, LIAO Bo-shou, JANG Hui-fang* . Identification of SSR makers associated with bacterial wilt resistance of peanut through correlation analysis[J]. 中国油料作物学报, 2015, 37(6): 803-.

References

[1] 廖伯寿. 花生在中国食物安全和农业发展中作用的经济学分析[J]. 粮食与油脂, 2001 (10): 28-29.

[2] 万书波. 我国花生产业面临的机遇与科技发展战略[J]. 中国农业科技导报, 2009, (1).

[3] 谈宇俊, 廖伯寿. 国内外花生青枯病研究述评[J]. 中国油料作物学报, 1990, (4).

[4] 任小平, 姜慧芳, 廖伯寿. 花生抗青枯病分子标记研究[J]. 植物遗传资源学报, 2008, 9(2): 163-167.

[5] 姜慧芳, 任小平, 雷永, 等. 花生青枯病抗性分子标记的初步研究[J]. 花生学报, 2004, 32(B11): 319-323.

[6] 唐荣华, 周汉群,花生属栽野杂种后代抗青枯病研究[J].中国油料作物学报,2000,22(3):61-65.

[7] 崔富华, 赖明芳,曾孝平.花生青枯病的分布及防治对策研究[J].花生学报,2003,32(4):17-22.

[8] 姜慧芳, 陈本银, 任小平,等. 利用重组近交系群体检测花生青枯病抗性SSR标记[J]. 中国油料作物学报, 2007, 29(1):26-30.

[9] 彭文舫, 姜慧芳, 任小平,等. 花生AFLP遗传图谱构建及青枯病抗性QTL分析[J]. 华北农学报, 2010, 25(6):81-86.

[10] 洪彦彬, 温世杰, 钟旎,等. SSR标记与花生青枯病、锈病抗性的相关性研究[J]. 广东农业科学, 2011. DOI:10.3969/j.issn.1004-874X.2011.z1.017.

[11]禹山林. 中国花生品种及其系谱[M]. 上海科学技术出版社, 2008.

[12] Cuc L M, M ace E S, Crouch J H, et al. Isolation and characterization of novel microsatellite markers and their application of diversity assessment in cultivated ground-nut(Arachis hypogaea L. ) [ J]. BMC Plant Biol 2008,8: 55.

[13] Shirasawa K, Bertioli D J, Varshney R K, et al. Integrated consensus map of cultivated peanut and wild relatives reveals structures of the A and B genome of Arachis and divergence of the legume genome[J]. DNA research, 2013, doi:10.1093/dnares/dss042.

[14] Kenta Shirasawa, Padmalatha Koilkonda, Koh Aoki. In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut[J]. BMC Plant Biology 2012, 12:80.

[15] Ravi K, Vadez V, Isobe S, et al. Identification of several small effect main QTLs and large number of epistatic QTLs for drought tolerance in groundnut (Arachis hypogaea L.) [J]. Theor Appl Genet 2011;122:1119-1132.

[16] 姜慧芳, 段乃雄. 花生种质资源描述规范和数据标准[ M ]. 北京: 中国农业出版社, 2006.

[17] Nei M．Analysis of gene diversity in subdivided populations［J］．Proc Natl Acad Sci USA，1973，70: 3321-3323.

[18] 陈本银, 姜慧芳, 廖伯寿等. 中国花生青枯病抗性遗传改良研究进展[J]. 中国农学通报, 2007, (8):369-372.

[19] 廖伯寿, 李文溶, 孙大容. 花生青枯病抗性遗传研究[J]. 中国油料作物学报, 1986, (3).

[20] 杨鑫雷, 周晓栋, 刘恒蔚等. AFLP 标记与棉花重要农艺性状的关联研究. 棉花学报, 2013, 25: 211–216

[21] 周金超, 杨鑫雷, 崔顺立等. 花生SSR标记与农艺性状的相关性[J]. 作物学报, 2014, (7)

[22] Shirasawa K, Bertioli D J, Varshney R K, et al. Integrated Consensus Map of Cultivated Peanut and Wild Relatives Reveals Structures of the A and B Genomes of Arachis and Divergence of the Legume Genomes[J]. DNA Research, 2013, 20(2):173-184.