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中国油料作物学报 ›› 2015, Vol. 37 ›› Issue (6): 803-.doi: 10.7505/j.issn.1007-9084.2015.06.010

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Identification of SSR makers associated with bacterial wilt resistance of peanut through correlation analysis

  

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
  • Online:2015-12-28 Published:2016-03-07

Abstract:

 Bacterial wilt caused by Ralstonia solanacearum is one of the important diseases limiting the production of peanut (Arachis hypogaea L.) in China. In the present study, 72 polymorphic SSR markers were used to detect the genetic diversity of 57 peanut cultivars with various resistance to bacterial wilt (BW). The results showed that the coefficients of genetic similarity between two genotypes among the involved 57 cultivars varied from 0.4978 to 0.9607 with the average of 0.7038. According to UPGMA cluster analysis, the involved 57 cultivars were clustered into two groups when the threshold valve of coefficients of genetic similarity was 0.69. Group I contained 30 accessions including 23 hypogaea genotypes and 7 vulgaris genotypes. 29 of the 30 accessions in group I were susceptible to BW. Group II composed of 27 vulgaris genotypes, among which, 26 were resistant to BW and one was susceptible. The clustering result of the involved 57 cultivars by SSR data was consistent with the botanical type and BW resistance of the cultivars. The genetic diversity among cultivars with BW resistance was slightly greater than that of the susceptible cultivars involved in the present study. Combining the data from bacterial wilt evaluation of the genotypes in two years, a total of 10 SSR loci related to BW resistance were detected through correlation analysis and multiple linear regression analysis, among which, 3 loci including 1B9-3, IPAHM288-1 and ARS590-1 were highly significantly associated with BW resistance. These 3 highly significantly associated with BW resistance makers were used to detect the RIL population from crossing of parents with BW resistance and susceptible respectively, loci 1B9-3 and ARS590-1 were detected in 62.85% and 40.00% of the BW resistant lines.

Key words: Peanut cultivars, Bacterial wilt resistance, Genetic diversity, SSR