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induced expressions of BnICS1 gene from Brassica napus and related signaling pathway
PENG Qi, GAO Jian-qin, ZHOU Xiao-ying, ZHANG Jie-fu, QI Cun-kou, PU Hui-ming, CHEN Song*
CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2016, Vol. 38 ›› Issue (01) : 7.
PDF(1566 KB)
PDF(1566 KB)
induced expressions of BnICS1 gene from Brassica napus and related signaling pathway
Key enzyme of SA (salicylic acid) synthesis is isochorismate synthase 1 (ICS1). To better understand the function of this enzyme gene in Brassica napus against infection by Sclerotinia sclerotiorum, cDNA of BnICS1 was cloned based on homologous sequence in B. rapa. The complete open reading frame of BnICS1 is 1 719 bp long, encoding 572 amino acids with a predicted chorismate-bind domain. Real-time RT-PCR showed that BnICS1 expression increased at 6hpi (hours post-inoculation), but decreased after 24hpi. SA signaling pathway genes were induced. MPK4 expression decreased at 6hpi, but increased after 24hpi. These revealed that SA synthesis was induced by S. sclerotiorum at the beginning, but suppressed after necrotic lesions appeared. Expressions of EDS5 and PR1 were increased from 6hpi, and PDF1.2 was decreased after inoculation. Results indicated that SA signaling pathway eventually led to necrotic lesions, while jasmonic acid pathway was inhibited.
Brassica napus L., Isochorismate synthase / Sclerotinia sclerotiorum / Salicylic acid (SA) signaling pathway / Cloning / Gene expression {{custom_keyword}} /
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