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中国油料作物学报 ›› 2016, Vol. 38 ›› Issue (01): 104-.doi: 10.7505/j.issn.1007-9084.2016.01.016

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Isolation and identification of IAA-producing strains from peanut rhizosphere and its promoting effects on peanut growth

  

  1. 1.College of Resources and Environment, Henan Agricultural University, Zhengzhou 450002, China;
    2. Soil and Fertilizer Station of Shandong Province, Ji’nan 250100, China;
    3. College of Resources and Environment, Southwest University, Chongqing 400715, China
  • Online:2016-02-28 Published:2016-05-04

Abstract:

 Some plant rhizosphere bacteria can secrete indoleacetic acid (IAA) to promote the plant growth and increase crop yield. This research aimed to screen IAA-producing strains from rhizosphere of peanut in sandy soil, and to analyze their promoting effect. 5 IAA-producing strains were isolated from rhizosphere of a healthy peanut plant. Among them, strain HS10 had the highest IAA-production ability and was identified as Bacillus tequilensis by 16S rRNA gene sequencing combined with its physiological and biochemical evaluation. HS10 produced maximum IAA at 24h of incubation with optimum conditions as 30℃, pH8, fluid volume 25 mL/250 mL, fructose and potassium nitrate as carbon source and nitrogen source respectively. Pot experiment demonstrated that HS10 significantly promoted plant growth and root system of peanut compared to the control. 

Key words:  Peanuts, Rhizosphere IAA-producing strains, Indoleacetic acid, Sandy chao soil, Pot experiment