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Resistance SNP development to downy mildew in Brassica napus using SLAF-seq technique
WANG Wei, LIU Fan, REN Li, XU Li, CHEN Wang, ZENG Ling-yi, HUANG Bing-wen, FANG Xiao-ping*
CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2016, Vol. 38 ›› Issue (5) : 555.
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PDF(1557 KB)
Resistance SNP development to downy mildew in Brassica napus using SLAF-seq technique
Downy mildew of Brassica napus, caused by the oomycete Hyaloperonospora parasitica, is an important disease during the epidemic. In this study, F2 population derived from a susceptible-parent and resistant-parent was used to construct resistant and susceptible pools. In order to obtain molecular markers related to disease resistance to downy mildew, BSA and SLAF-seq technique were developed. Firstly, reference genome of B. napus was used to design marker discovery experiments in silico by simulating the number of markers produced by different enzymes. Secondly, the SLAF library was conducted and sequenced by paired-end sequencing. 132 519 SLAF labels were obtained and 264 256 SNPs were found. Thirdly, the associated region was located by SNP-index, and the SNPs locating at the associated region were analyzed between the two parents. After annotation, 2 non-synonymous -coding SNPs were found. By verification, these SNPs were considered to be related to resistance to downy mildew and were genetically stable. Finally, 2 specific primer pairs for each SNP were obtained by SNAPER software which designed primers generated differences in the amplification products.
Brassica napus L. / Downy mildew / SLAF / Marker / SNP {{custom_keyword}} /
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