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中国油料作物学报 ›› 2016, Vol. 38 ›› Issue (5): 563-.doi: 10.7505/j.issn.1007-9084.2016.05.004

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Selection of core SSR markers and identification of fingerprint on peanut cultivars

  

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of Agriulctural Sciences, Wuhan 430062, China
  • Online:2016-10-28 Published:2017-01-09

Abstract:

pAccording to the location of SSR primers in genetic linkage map, 200 pairs of primers were selected with uniform distribution on the chromosome. Then, through olyacrylamide gel electrophoresis and silver stain technology, 120 primers were chosen with clear bands and repeating. Finally, using capillary electrophoresis technology to screen, primers with uniform distribution on the chromosome were selected with the alleles number more than 4, and polymorphism information content (PIC) value higher than 0.4, and heterozygosity being lower than 0.1 primers. 60 of primers were selected to identify fingerprint of peanut cultivars as core primers, which had abundant polymorphism, broad representative and uniform distribution of genome, with good amplification effect in both common primers and fluorescent primers. Among 60 core primers, 352 polymorphic alleles were amplified in 100 peanut varieties with the average polymorphic alleles being 5.87, and the average number of genotypes distinguished being 6.35, and the mean PIC being 0.54. Primers accounted for 66.67% with high polymorphism primers, and heterozygosity was below 0.06. The genetic similarity coefficient among cultivars ranged from 0.530 to 0.683. Fingerprint establishment of 100 peanut varieties provided a foundation for the construction of DNA fingerprint database of peanut varieties and resources.

Key words:  Peanut (Arachis hypogaea L.), Fluorescent SSR markers, Capillary electrophoresis, Fingerprint