Welcome to CHINESE JOURNAL OF OIL CROP SCIENCES,

中国油料作物学报 ›› 2016, Vol. 38 ›› Issue (6): 737-.doi: 10.7505/j.issn.1007-9084.2016.06.005

Previous Articles     Next Articles

 Breeding strategy of temporary maintainer with the same genetic background as two-purpose line of recessive epistatic genic male sterility system in Brassica napus L.

  

  1.  Crop Research Institute, Anhui Academy of Agricultural Sciences; Hefei Rapeseed Subcenter, National Oil Crop Improvement Center; Anhui Provincial Key Laboratory of Crop Quality Improvement, Hefei 230031, China
  • Online:2016-12-28 Published:2017-03-06

Abstract:

Newbreedingstrategyoftemporarymaintainerwiththesamegeneticbackgroundastwo-purpose line9012ABwasproposedbasedonthecharacteristicsofrecessiveepistaticgenicmalesterilitysysteminBrassica napusL.Afterhightemperaturetreatment,thesterileplantswithhomozygousgenotypewasstable,andthefertility
alterationwasobservedonthesterileplantswithheterozygousgenotypeandthefertilealterantplantscouldselfwith certainsuccess.Backcrosswasmadebetweensterileplantsfrom9012ABasrecurrentparentandtemporarymain tainer204TAMasdonorparent.F1,BC1-2progenieswereallsterile,andsimilarplantsto9012ABwereselected andtreatedcontinuouslyfor15hoursat35℃ withlight.Fertilealterantplantswerethenbackcrossedtosterile plantsfrom9012AB.SelfingprogenyofselectedfertilealterantplantswasobtainedfromBC3.Temporarymaintai ner9012TAMwiththesamegeneticbackgroundas9012ABwasdevelopedbasedontheoffspringofselectedfertile individualsfrommentionedselfingprogeny.

Key words:  Brassica napus L., Recessive epistatic genic male sterility, Temporary maintainer, Two-purpose line