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中国油料作物学报 ›› 2016, Vol. 38 ›› Issue (6): 771-.doi: 10.7505/j.issn.1007-9084.2016.06.010

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Expression of functional genes related to fatty acid synthesis of oil flax

  

  1. 1. College of Life Science, Hohhot 010018, China; 2. Agronomy college, Hohhot 010018, China; 3. College of Ecology and Environment, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Online:2016-12-28 Published:2017-03-06

Abstract:

To better understand the relationship between fatty acid desaturation enzyme gene expression and fatty acid composition of leaves and capsule balls, 3 varieties of oil flax was investigated as materials. Real-time fluorescence quantitative PCR was applied to investigate relative expression of these 4 genes (phosphatidylcholine diacylglycerol cholinephosphotransferase 1 gene PDCT1, phosphatidylcholine diacylglycerol cholinephosphotransferase 2 gene PDCT2, fatty acid desaturases 2a gene FAD2a, and fatty acid desaturases 3a gene FAD3a). The relationship between relative expression and fatty acid composition was discussed. Results showed that these 4 genes expression patterns followed the normal distribution in leaves and capsule balls during development. At linseed forming period, these 4 genes were the major genes at high expression levels. Expression quantity was significantly correlated among genes (correlation coefficiency r ranged from 0.989 to 0.643, P<0.01). At linseed mature stage, gene expressions were significantly correlated with fatty acid composition. The significant positive correlation coefficiency were 0.548 to 0.405 with stearic acid, and 0.494 to 0.304 with linolenic acid. The significant negative correlation coefficiency was -0.299 to -0.497 with linoleic acid. The expression of PDCTs, FAD2a and FAD3a genes changed fatty acid compositions and led to oil quality change in oil flax.

Key words:  Oil flax, Real-time fluorescence quantitative PCR, Fatty acid desaturase gene (FAD), Phosphatidylcholine diacylglycerol cholinephosphotransferase gene (PDCT)