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中国油料作物学报 ›› 2017, Vol. 39 ›› Issue (1): 30-.doi: 10.7505/j.issn.1007-9084.2017.01.005

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Development of high oleic acid peanut from molecular marker assisted selection

  

  1. 1. Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100;
    2. Kaifeng Academy of Agriculture and Forestry, Kaifeng 475004
  • Online:2017-02-28 Published:2017-05-08

Abstract:

To breed high oleate peanut cultivars suitable for Shandong and Huang-Huai-Hai region of China, HY23×DF12(SAAS-01), HY31×KN176(SAAS-02)and HY31×Sunoleic95R, three cross combinations were made using two elite peanut cultivars Huayu 23 and Huayu 31 as female parents, and three high oleate peanuts Sunoleic95R, DF12 and Kainong176 as male parents. One cross, four backcrosses and one selfing were carried out within three years. The progenies of each cross were examined by CAPS (cleaved amplified polymorphic sequence) and sequencing of PCR products. KASP (kompetitive allele specific PCR) was performed for more efficient examining of BC4F2 genotypes. The numbers of BC4F2 seeds with aabb genotype of three combinations SAAS-01, SAAS-02 and SAAS-03 were 1, 18 and 26, respectively. Different numbers of BC4F2 seeds with Aabb, aaBb and AaBb genotypes were obtained. These seeds could be grown and selfed to obtain the aabb homozygous. These materials could provide useful resources for breeding of high oleic acid peanut cultivars with high yield and suitable for Shandong and Huang-Huai-Hai region.

Key words: peanut, high oleic acid, marker assisted selection, backcross, breeding