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中国油料作物学报 ›› 2017, Vol. 39 ›› Issue (6): 763-.doi: 10.7505/j.issn.1007-9084.2017.06.006

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Identification of QTL and consistency QTL with resistance to Sclerotinia sclerotiorum in soybean

  

  1. Soybean Research Institute, Northeast Agricultural University, Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, China
  • Online:2017-12-28 Published:2018-03-19

Abstract:

A total of 128 lines derived from a cross between a susceptible soybean cultivar Hefeng 25 and a disease resistant soybean cultivar Maple Arrow, were analyzed through isolated leaf stalk inoculation method and living stem inoculation method. A total of 7 QTLs were detected through combining 2 sets of phenotypic identification data with the interval mapping method. QTL for the soybean with pests or disease-resistance was collected through meta-analysis, the consistent QTL map for the soybean with pests or disease-resistance was constructed, and 2 consistent QTL were obtained. The more reduced confidence interval was obtained and 21 candidate genes were discovered, 3 of which were associated with disease resistance. The consistent results were similar to that of this study in B1 linkage group, which provided a research basis for fine positioning and gene cloning.

Key words: Soybean, Sclerotinia sclerotiorum, QTL mapping, Meta-analysis, Consistency QTL