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中国油料作物学报 ›› 2017, Vol. 39 ›› Issue (6): 794-.doi: 10.7505/j.issn.1007-9084.2017.06.010

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Morphological variation and SRAP analysis of flax core germplasm resources

  

  1. 1. Institute of Agronomy, Inner Mongolia Agriculture University, Hohhot 010019, China; 2. Inner Mongolia Academy of Agriculture and Husbandry Sciences, Hohhot 010031, China
  • Online:2017-12-28 Published:2018-03-19

Abstract:

The phenotypic variation and genetic diversity of 401 domestic and foreign flax germplasm resources were studied, by multi-statistical and SRAP marker methods. The results showed that the average variation coefficient was 17.87%, the average genetic diversity index was 2.132. There were significant differences among 28 pairs of traits. 26 pairs of SRAP primers, were used to evaluate the flax core collection, and 234 polymorphism bands were generated, with the average polymorphism information content (PIC) for each pair of primers being 0.60. The effective number of alleles (Ne), aromatic information index (I) and Nei's genetic identity (H) were 1.5847, 0.5235 and 0.3479 at the species levels, and 1.5105, 0.4644 and 0.3054 at the group levels, respectively. According to the cluster analysis, 401 flax resources could be divided into 2 categories when the genetic similarity coefficient (GS) was 0.295, and into 7 sub-types when GS was 0.375, being roughly consistent with 7 different origins. The studies showed that the agronomic traits variation was greater than that of quality traits for studied flax germplasm resources. There were great genetic differences between domestic and foreign varieties (or lines), showing a distant kinship; Geography was the main factor affecting the flax germplasm genetic diversity.

Key words:  oil flax, germplasm resources, phenotypic traits, genetic diversity, SRAP