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中国油料作物学报 ›› 2018, Vol. 40 ›› Issue (1): 25-.doi: 10.7505/j.issn.1007-9084.2018.01.004

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Cloning and functional analysis of a novel early embryo-specific expressed AhDGAT3 promoter from Arachis hypogaea L.

  


  1. Industrial Crops Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture, P R China / Henan Provincial Key Laboratory for Oil Crops Improvement / National and Provincial Joint Engineering Laboratory for Peanut Genetic Improvement, Henan Province, Zhengzhou 450002, China
  • Online:2018-02-28 Published:2018-05-15

Abstract:

To characterize an early embryo-specific promoter, the putative promoter region of AhDGAT3 (GenBank: AY875644.1) was isolated by nested PCR-based genomic walking method. Results showed that the 2181 bp 5´ flanking sequence of AhDGAT3 was isolated. Sequence analysis in PLACE and plantCARE database identified the core regulatory elements such as TATA-box and CAAT-box, the putative cis-acting regulatory elements involved in MeJA-responsiveness, defense stress responsiveness, light-responsiveness, heat stress responsiveness and further the seed or embryo-specific elements. For the functional and characteristic analysis of AhDGAT3 promoter, the recombinant vector pBI121-PAhDGAT3 was designated and transformed into Arabidopsis. Histochemical staining revealed that the activity of pAhDGAT3 was restricted to a short temporal and spatial window from the heart stage to torpedo stage of the embryo.

Key words: Peanut (Arachis hypogaea L.), DGAT3, Early embryo, Seed-speci?c promoter, GUS reporter gene