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中国油料作物学报 ›› 2018, Vol. 40 ›› Issue (1): 127-.doi: 10.7505/j.issn.1007-9084.2018.01.016

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Functionality of BnWRKY70 gene of oilseed rape (Brassica napus L.) in relation to blackleg

  

  1. 1. Plant Protection Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031, China; 2. College of Life Science, Inner Mongolia University, Hohhot 010021, China; 3. College of Life Science , Inner Mongolia Agricultural University, Hohhot 010019, China
  • Online:2018-02-28 Published:2018-05-15

Abstract:

WRKY transcription factors is the important member of plant signal transduction pathway, plays an important role in the process of plant resistance, in order to better understand BnWRKY70 in rapeseed blackleg disease resistance. The sequence of BnWRKY70 specific gene to response to blackleg was analyzed, and the 308 bp CDS specific section of BnWRKY70 gene cloned, using the Tobacco rattle virus carrier constructed induced gene silencing system of oilseed, then validated target gene expression quantity with qRT - PCR, finally evaluation resistance of BnWRKY70 gene silencing rape after inoculation of blackleg pathogens by phenotypic observation and statistical analysis of disease index. The results showed that BnWRKY70 gene had the longest ORF of 858 bp, encoding 285 amino acids, containing a WRKY conservative domain structure, belonging to the class Ⅲ WRKY transcription factors, and BnWRKY70 gene expression was significantly decreased after silence by successful VIGS system constructed in oilseed rape, the leaf of gene silencing oilseed rape is more susceptible than target gene silence oilseed rape, the disease spot area is larger, and the disease index was significantly higher than that of control. It all showed that the silence of BnWRKY70 genes decreased the oilseed rape resistance, to further reveal pathogen molecular interactions mechanism of oilseed rape.

Key words:  Brassica napus L., blackleg, VIGS, BnWRKY70 gene