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中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (2): 192-.doi: 10.7505/j.issn.1007-9084.2019.02.005

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Effect of exogenous 5-hydroxytryptamine (5-HT) on rape (Brassica napus L.) seedling under drought stress

  

  1. 1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; 
    2. Zhangye Academy of Forestry Science, Zhangye 734000, China; 
    3.Hubei Vocational College Of Bio-Technology, Wuhan 430070, China;
    4. Agricultural Technology Promotion Center in Qichun County, Qichun 435300, China;5. Oil Crop Station, Agricultural Bereau of Xishui County, Xishui 438200, China
  • Online:2019-04-28 Published:2019-07-04

Abstract:

 5-hydroxytryptamine (5-HT, serotonin) is a naturally occurring compound in plants. To reveal the effects of 5-HT on rape (Brassica napus L.) seedlings under drought, effect of exogenous 5-HT on rape (Brassica napus L.) growth under PEG stress were investigated. The traits of seedling growth, active oxygen metabolism and osmotic adjustment substance were evaluated. Results showed that the simulative drought inhibited seedling growth, resulted in H2O2 and malonadialdehyde (MDA) accumulation, increased proline (Pro) and soluble sugar (SS) content, and significantly enhanced the activity of catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX). Under the simulative drought condition, exogenous 5-HT alleviated the inhibition on seedling growth. The activity of antioxidant enzymes (CAT, SOD and APX) was improved significantly by exogenous 5-HT, and the content of H2O2 and MDA was decreased. Results also showed that solute accumulation under the stress was significantly enhanced with exogenous 5-HT by increasing the content of Pro and SS. This study indicated that exogenous 5-HT couldan improve H2O2-scavenging capacity of rape seedlings by enhancing the activity of antioxidant enzymes and also alleviate osmotic stress by promoting the accumulation of osmoregulatory substances. Ultimately, exogenous 5-HT improved the biomass of rape seedlings under drought stress, therebyand effectively alleviateding drought stress in rape seedlings.

Key words: rape (Brassica napus L.), 5- hydroxytryptamine (serotonin), simulative drought, seedling