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中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (3): 383-.doi: 10.7505/j.issn.1007-9084.2019.03.010

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Cloning and expression analysis of GmCYP82C4 gene in soybean

  

  1. 1. Institute of Pomology, Chinese Academy of Agricultural Sciences,Xingcheng 125100, China;
     2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences,Beijing 100081, China
  • Online:2019-06-28 Published:2019-09-02

Abstract:

Cytochrome P450 CYP82 is in a unique gene family in dicotyledonous plants. To better undrstand its important roles in plant growth and response to biotic and abiotic stresses, soybean gene GmCYP82C4 was homologously cloned and identified. The gene was 1584 bp long, encoding 527 amino acids with a conservative heme-binding domain. Bioinformatics showed that GmCYP82C4 protein was homology with PsCYP82A1 in pea. Subcellular localization showed that GmCYP82C4 was located in plasma membrane. Real-time fluorescence quantitative PCR showed that GmCYP82C4 expression in roots of soybean seedlings was the highest. GmCYP82C4 expression was induced by low temperature, wound (cut) and Phytophthora sojae infection respectively. GmCYP82C4 was responsive to plant hormones as ABA, MeJA (methyl jasmonate), ethylene and SA (salicylic acid). These results suggested that GmCYP82C4 might regulate the response of soybean to stress through hormonal signaling pathway. 

Key words: soybean, cytochrome P450, GmCYP82C4, plant hormone, cold stress, wound, Phytophthora sojae infection