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中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (3): 445-.doi: 10.7505/j.issn.1007-9084.2019.03.017

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Allelopathy autotoxicity of aqueous extracts of oil flax stubble

  

  1. Biotechology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
  • Online:2019-06-28 Published:2019-09-02

Abstract:

 In order to investigate the allelopathy autotoxicity of oil flax stubble and identify the organic compounds responsible for its autotoxicity, aqueous extracts of stubble root, stem and leaf were prepared respectively. Different concentrations of gradient aqueous extracts were set for seed germination and seedling growth experiments. The autotoxic effects were determined by measuring germination potential, germination rate, seeding length and root weight, etc; and the main autotoxic chemical substances of the stubble root, stem and leaf were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the stubble root, stem and leaf aqueous extracts of oil flax inhibited the seed germination and seedling growth in a dose-dependent manner. With the increase of aqueous extract concentration, the aqueous extract from the stubble leaves reached the critical concentration first, followed by the aqueous extract of stubble stem, and finally the aqueous extract of the stubble root. The synthesis effect showed that the relationship between the aqueous extract of different stubble organs and the autotoxicity is that stubble leaf > stubble stem > stubble root. Eight, twenty-one and fourteen compounds were identified in aqueous extracts of root, stem and leaf, respectively, including acids and esters. The most abundant autotoxins was palmitic acid. These results showed that aqueous extracts of oil flax stubble significantly affecteds seed germination and seedling growth, which maycould be one of the main cause of continuous cropping obstacles of oil flax.

Key words: oil flax (Linum usitatissimum L.), stubble, aqueous extracts, autotoxicity, GC-MS, allelochemicals, continuous cropping obstacle