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中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (3): 349-.doi: 10.7505/j.issn.1007-9084.2019.03.06

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Difference of DNA methylation in winter rapeseed (Brassica napus L.) with temperature sensitivity and prediction of At4g02000-like protein

  

  1. Gansu Provincial Engineering Research Center of Rapeseed, Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Improvement and Key Laboratory of Crop Genetics and Planting Innovation of Gansu Province, Lanzhou 730070, China
  • Online:2019-06-28 Published:2019-09-02

Abstract:

 To better understand the cold-resistance of winter rapeseed (Brassica napus L.), DNA methylation were investigated using MSAP (methylation sensitive amplified polymorphism) technology on 13 materials with different cold resistance under low temperature (4oC) stress. MSAP results showed that, after 4oC treatment, the weak cold-resistance lines (14 Meiqieshi 7, 14 Meiqieshi 16, 14 Meiqieshi 20, 14 Meiqieshi 3 and Meiqieshi 38 had increased DNA methylation level and had higher methylation degree. The strong cold-resistant lines (15TS306, 14NS52-3, 15TS309, 14NS54-7 and 15TS312) had higher degree of demethylation. Sequence analysis of the differentially methylated fragments showed that 16 of 22 fragments were similar to the known and putative functional enzymes. Among them, At4g02000-like protein had the greatest variation. Cloning and bioinformatics results showed that its isoelectric point was 9.49 and the relative molecular weight was 39.2525kD. In sumarry, after low temperature stress, the strong cold-resistant lines had decreased DNA methylation level with dominant demethylation, while the weak cold-resistance lines had increased DNA methylation level with dominant methylation. In these winter rapeseeds, cold adaptability showed close correlation between differentially methylation patterns of some specific genes. 

Key words:  Brassica napus, cold stress, DNA methylation, MSAP