中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (4): 497-.doi: 10.7505/j.issn.1007-9084.2019.04.003

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Construction of core collection of polima CMS restorer lines in Brassica napus L. based on SLAF sequencing


  1. Academy of Agricultural and Forestry Sciences, Qinghai University, Key Laboratory of Spring Rapeseed Genetic Im? provement/The Qinghai Research Branch of the National Rapeseed Genetic Improvement Center, Spring Rape Scientif? ic Observation Experimental Station of Ministry of Agriculture and Rural Areas, Qinghai Research and Development Center for Spring Rapeseed, Xining 810016, China
  • Online:2019-08-28 Published:2019-10-18


In order to provide a foundation base for conservation, selection, heterosis and hybridization as well as for utilizing germplasm,118 Brassica napus of Polima CMS restorer lines were studied and sequenced through spe? cific length amplified fragment sequencing (SLAF-seq). By developing SNP markers and analyzing genetic relation?ships, core collections were conducted. A number of 2516 effective SNP loci were developed. 118 Brassica napus L. restorer lines were used to construct core collection and their genetics were analyzed, based on these effective SNP markers. Results showed that the average Nei’s genetic distance between these restorer lines was 0.319, different genetic components of restore lines had a large genetic distance. 118 restorer lines were clustered into five catego? ries, most of the materials with the same origins were classified into the same category. The core collection of Brassi? ca napus had two types of qualified varieties, containing 34 and 46 lines respectively, which named C30 and C40. By evaluating these two core collections, the allelic coverage of these two subsets was more than 99.8% and the MR (Rogers distance) and CE (Cavali-Edward distance) genetic distance indices were similar, while the polymorphic
content reached 0.28. The core germplasm of the constructed Brassica napus L. restorer lines met the requirement of core collection, and could represent the original species.

Key words:  , Brassica napus L.;polima CMS restorer lines;SNP markers;SLAF tags;core collection