中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (4): 517-.doi: 10.7505/j.issn.1007-9084.2019.04.005

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Cloning and functional analysis of soybean GmWRI1a promoter


  1. Northeast Agricultural University / Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Harbin 150030, China
  • Online:2019-08-28 Published:2019-10-18


 To explore the promoter function of GmWRI1a gene, a sequence of 1669 bp was cloned from the up? stream of GmWRI1a gene from soybean (Glycine max (Linn.) Merr.) cv Dongnong 50. GUS reporter gene showed the active promoter sequence by visible GUS expression in transgenic Arabidopsis. According to the predicted locations of cis-acting elements of the promoter, 4 promoter 5’-deletion-fragments were amplified and tested. In transformed Arabidopsis, all 4 fragments exhibited GUS blue. Moreover, by GUS enzyme activity tests, the promoters contained ethylene, jasmonic acid and gibberellin hormone response elements. The cis-acting element of ethylene located be? tween -1138 and -1087 bp, the cis-acting element of jasmonic acid located between -1087 bp and -690 bp, and the cis-acting response of gibberellinla located between -690 bp and -437 bp.

Key words: soybean, GmWRI1a promoter, cis-acting element, hormone response, GUS enzyme activity analysis