Cloning and function of GsWRKY57 transcription factor gene response to drought stress #br#

doi:10.7505/j.issn.1007-9084.2019.04.006

Abstract

Abstract:

The WRKY transcription factor is an important transcription factor found in plants in recent years, it plays an important role in the process of plant stress resistance. In order to analyze the mechanism of GsWRKY57 transcription factors in the process of resistance response, a full –length CDS of GsWRKY57 gene was cloned by an? alyzing wild soybean leaf transcriptome data. Sequence analysis showed that GsWRKY57 cDNA contained 903bp ORF encoding a protein of 300 amino acids residues with molecular mass of 34.23 kD and theoretical pI of 5.88. Gs? WRKY57 protein contained a WRKY conservative domain structure, belonging to the class III WRKY transcription factors. Phylogenetic tree analysis demonstrated GsWRKY57 shared the highest homology with Glycine max, fol?
lowed by Vigna angularis, Cajanus cajan. The promoter analyses identified the putative cis-acing regulatory ele? ments of GsWRKY57. Tissue specific expression analysis indicated GsWRKY57 was ubiquitously expressed in leaves, stems, flowers, pods and roots, with the highest level in leaves and the lowest in root. Its expression was sig? nificantly induced by jasmonate, salicylic acid, abscisic acid and drought. Over-expression of GsWRKY57 increased drought stress tolerance in transgenic Arabidopsis lines.

Key words: Glycine soja, GsWRKY57, transcription factor, overexpression, drought stress

WANGYan-yan，ZHANGYong-xing，GUOWei，DAIWen-jun,ZHOUXin-an,JIAOYong-qing*,SHENXin-jie *. Cloning and function of GsWRKY57 transcription factor gene response to drought stress #br#[J]. 中国油料作物学报, 2019, 41(4): 524-.

References

［1］张秋平，杨宇红，谢丙炎，等. 植物转录因子的超表达及其在分子育种中的应用［J］. 分子植物育种，2006， 4（3）：411-418.
［2］王娜，张振葆，黄凤珠，等. WRKY 转录因子参与植物非生物胁迫应答的研究进展［J］. 核农学报，2014，28 （10）：1 819-1 827.
［3］ Eulgem T，Rushton P J，Robatzek S. The WRKY super? family of plant transcription factors［J］. Trends Plant Sci? ence，2000，5：199-206.
［4］ Rushton P J，Torres J T，Parniske M，et al. Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes
［J］. EMBO J，1996，15（20）：5 690-5 700. ［5］ Rocher A，Dumas C，Cock J M. A W-box is required for full expression of the SA-responsive gene SFR2 ［J］. Gene，2005，344（1）： 181-192.
［6］ Kim C Y，Zhang S. Activation of a mitogen-activated protein kinase cascade induces WRKY family of tran? scription factors and defense genes in tobacco［J］. Plant J，2004，38（1）：142-151.
［7］ Pandey S P，Somssich I E. The role of WRKY transcrip? tion factors in plant immunity ［J］. Plant Physiology， 2009，150（4）：1648-1655.
［8］ Vivian E V，Carlos B，Luciano C M，et al. Activation of rice WRKY transcription factors：an army of stress fight? ing soldiers［J］？ Curr Opin Plant Biol，2018，45：1-8.
［9］ Aditya B ，Aryadeep R . WRKY proteins：signaling and regulation of expression during abiotic stress responses ［J］. Sci World J，2015，2015：1-17.
［10］张凡，尹俊龙，郭瑛琪，等. WRKY 转录因子的研究进展［J］. 生物技术通报，2018，34（1）：40-48.
［11］ Asai T，Tena G，Plotnikova J，Willmann M R，et al. MAP kinase signalling cascade in Arabidopsis innate im? munity［J］. Nature，2002，415（6875）：977-983.
［12］ Jiang Y，Qiu Y，Hu Y，et al. Heterologous Expression of AtWRKY57 confers drought tolerance in Oryza sativa ［J］. Plant Science，2016，7：145.
［13］ Robatzek S，Somssich I E. A new member of the Arabi? dopsis WRKY transcription factor family，AtWRKY6，is associated with both senescence- and defense-related processes［J］. Plant J，2001，（28）：123-133.
［14］李大红，王春弘，刘喜平，等. 大豆GmWRKY35 基因的克隆及其增强烟草耐旱能力研究［J］．大豆科学， 2017，36（5）：685-691.
［15］闫文飞，程凡生，姜新强，等. 野大豆盐碱胁迫相关Gs? TIFY6B 基因克隆及表达特性分析［J］．华农农学报， 2018，33（4）：82-89.
［16］史宏，刘学义. 野生大豆抗旱性鉴定及研究［J］．大豆科学，2003，22（4）：264-268.
［17］孙明哲，孙晓丽，于青跃，等，逆境诱导基因GsS? NARE1的耐逆功能分析［J］．中国农业科学，2013，46 （11）：2 191-2 200.
［18］陈洪国，桂花幼苗对不同程度水分胁迫的生理响应［J］．华中农业大学学报，2006，25（2）：190-193.
［19］ Livak K，Schmittgen T. Analysis of relative gene expres? sion data using real-time quantitative PCR and the 2（- Delta Delta C（T））method［J］. Methods，2001，25： 402-408.
［20］张家团，屈燕平. 近30年来中国干旱灾害演变规律及抗旱减灾对策探讨［J］. 中国防汛抗旱，2008（5）： 47-52.
［21］彭琦，高建琴，周晓婴，等. 甘蓝型油菜异分支酸合酶基因的克隆及信号通路相关基因的诱导表达［J］. 中国油料作物学报，2016，38（1）：7-12.
［22］钱金鑫，齐学军，解莉楠，等. 拟南芥中WRKY家族基因功能的研究进展［J］．安徽农业科学，2014（5）：1 295-1 297.
［23］ Qiu Y P，Yu D Q. Overexpression of the stress-induced OsWRKY45 enhances disease resistance and drought tol? 7 中国油料作物学报2019，41（2）
erance in Arabidopsis［J］. Environ Exp Bot，2009，65 （1）：35-47.
［24］ Yu S，Ligang C，Liping Z，et al. Overexpression of Os? WRKY72 gene interferes in the abscisic acid signal and auxin transport pathway ofArabidopsis［J］. J Biosci， 2010，35（3）：459-471.
［25］ Song Y，Jing S J，Yu D Q. Overexpression of the stressinduced OsWRKY08 improves osmotic stress tolerance in Arabidopsis ［J］. Chinese Science Bulletin，2009，54 （24）：4 671-4 678.
［26］ Birkenbihl R P ，Kracher B ，Somssich I E ，et al. Nega? tive regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100 ［J］. eLife，4，（2015-06-13），2015，4（e07295）.

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