Abstract:

To investigate the regulatory role of DDTC (sodium diethyldithiocarbamate) in antioxidant system of waterlogging stressed soybean, 2 Glycine max varieties Xudou 18 (sensitive) and Nannong 1138-2 (tolerant) were used. By the mode of 1 d after spraying DDTC at flowering stage followed by 8 d waterlogging, antiosidation system changes were investigated in roots at flowering stage with pot experiments. Results showed that under the stress, Nannong 1138-2 had decreased less in root dry mass, less membrane lipid peroxidation damage, and lower ROS (re?active oxygen species) levels, but more active on APX (ascorbate peroxdidase), GR (glutathione reductase) and DHAR (dehydroascorbate reductase), and its contents of ascorbic acid (AsA) and glultathione (GSH) were in? creased. It indicated that Nannong 1138-2 had stronger antioxidant capacity under waterlogging. The result showed that DDTC could further increase the activities of POD (peroxidase), APX, DHAR and GR. It led to increasing AsA content and AsA/DHA ratio in roots, and to significantly reducing root ROS levels and membrane lipid peroxidation damage. It suggested that DDTC increased the ROS-scavenging activity in roots by