Application of ISSR markers to reveal the genetic diversity of sunflower broomrape in China

doi:10.7505/j.issn.1007-9084.2019.04.018

Abstract

Abstract: Sunflower broomrape is parasitic seed plant seriously endangering the sunflower industry in China. To clarify the genetic relationship of sunflower broomrape in different regions of China, ISSR markers were used on population genetic diversity of 96 sunflower broomrape samples from different provinces in China. Results showed 12 primers with high polymorphism and good reproducibility screened from 100 ISSR primers. A total of 147 bands were amplified by PCR, of which 90 were polymorphic. The percentage of polymorphic bands was 61.2%. Shannon information index and Nei′s diversity index of broomrape population from different locations were both increased with the increase of population sample size. Among them, broomrapes form Inner Mongolia and Xinjiang provinces
showed higher polymorphism. Their Shannon indices were 0.5560 and 0.5067 respectively. Genetic clustering re? sults showed that the samples from 6 different provinces could be divided into 2 sub-groups. Shanxi, Hebei and Shaanxi samples were clustered into one sub-group; and those from Jilin, Xinjiang and Inner Mongolia were clus? tered into another sub-group. Broomrape samples between Hebei and Shaanxi had the closest relationship, while samples from Xinjiang and Hebei had the furthest relationship.

Key words: sunflower broomrape (Orobanche cumana Wallr.), ISSR markers, genetic diversity

SHI Bi-xian，ZHANG Jian，GU Yuan-guo，LAI Cheng-xia，LEI Zhong-hua，SHA Hong，GAO Yan， MAYILA Yusuyin，ZHAO Jun*. Application of ISSR markers to reveal the genetic diversity of sunflower broomrape in China[J]. 中国油料作物学报, 2019, 41(4): 629-.

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