[ 1 ] 　KiritsakisA K. Composition of olive oil and its nutritional
and health effect. Proceedings of the 10 th International
Rapeseed Congress[C ]. Sep t, 26, 1999. Wratten N. and
Salisbury P A, Eds Canberra Australia, GCIRC.
[ 2 ] 　Stoutjesdijk P A, Hurlstone C, Singh S P, Green A G.
High - oleic acid Australia Brassica napus and B. juncea
varieties p roduced by co - sup ression of endogenous delta
- 12 desaturases [ J ]. Biochem Soc Trans, 2000, 28:
938—940.
[ 3 ] 　官春云. 油菜转基因育种研究进展[A ]. 食物与能源
安全战略中的中国油料,中国作物学会油料作物专业
委员会第五届学术年会论文集[ C ]. 中国农业科学出
版社, 2004. 13—17.
[ 4 ] 　石东乔,周奕华,胡赞民,等. 基因枪法转移反义油酸
脱饱和酶基因获得转基因油菜[ J ]. 农业生物技术学
报, 2001, 9 (4) : 359—362.
[ 5 ] 　熊兴华,官春云,李　　,等. 基因枪法向甘蓝型油菜
转移反义FAD2基因的研究[ J ]. 湖南农业大学学报
(自然科学版) , 2003, 29 (3) : 188—191.
[ 6 ] 　L iu Q, Singh S, Green A. Genetic modification of cotton
seed oil using inverted - repeat gene - silencing tech2
niques[ J ]. Biochem Soc Trans, 2000, 28: 927—929.
[ 7 ] 　Stoutjesdijk P A, Singh S P,L iu Q, et al. hp - RNA - me2
diated targeting of the Arabidosis FAD2 gene gives highly
efficient and stable silencing[ J ]. Plant Physiology, 2002,
129 (4) : 1723—1731.
[ 8 ] 　Singh S, Green A, Stoutjesdijk P, et al. Inverted - repeat
DNA: a new gene - silencing tool for seed lip id modifica2
tion[ J ]. Biochem Soc Trans, 2000, 28 (6) : 925—927.
[ 9 ] 　Stoutjesdijk P A, Hurlstone C, Singh S P, et al. Genetic
manipulation for altered oil quality in Brassica. Proceed2
ings of the 10 th International Rapeseed Congress [ C ].
Sep t, 26, 1999. Wratten N. and Salisbury P. A. , Eds
Canberra Australia, GCIRC.
[ 10 ] 　Kinney A J. Development of genetically engineered soy2
bean oils for food app lications[ J ]. J Food L ip ida, 1996,
3: 273—292.
[ 11 ] 　Waterhouse P M, Graham M W,WangM B. Virus re2
sistance and gene silencing in p lants can be induced by
simultaneous exp ression of sense and anti - sense RNA
[ J ]. Proc NatlAcad Sci, 1998, 95: 13959—13964.
[ 12 ] 　Chuang C,Meyerowitz E. Specific and heritable genetic
interference by double - stranded RNA in Arabidop sis
thialiana [ J ]. Proc Natl Acad Sci, 2000, 97: 4985—
4990.
[ 13 ] 　中国科学院上海植物生理研究所,上海市植物生理
学会. 现代植物生理学实验指南[M ]. 科学出版社,
1999. 336.
[ 14 ] 　金冬雁,黎孟枫,译. 分子克隆实验指南[M ]. 第二
版,科学出版社, 1996. 19—21.
[ 15 ] 　熊兴华,官春云,李　　,等. 油菜种子特异表达nap in
基因启动子的克隆及序列分析[ J ]. 生物技术, 2003,
113 (13) : 4—5.
[ 16 ] 　Auld D L, Heikkinen M K, Erickson D A, et al. Rape2
seed mutantswith reduced levels of polyunsaturated fatty
acids and increased levels of oleic acid [ J ]. Crop Sci2
ence, 1992, 32: 657—662.
[ 17 ] 　和江明,王敬乔,陈　薇,等,用EMS诱变和小孢子培
养快速获得甘蓝型油菜高油酸种质材料的研究[ J ].
西南农业学报, 2003, 16 (2) : 34—36.
[ 18 ] 　KinneyA J. Genetic modification of the storage lip ids of
p lants[ J ]. CurrOp in Biotech, 1994, 5: 144—151.
陈松等:甘蓝型油菜种子特异性表达fad2基因的ihpRNA载体构建255
[ 19 ] 　王敬乔,陈　薇,曾黎琼,等. Δ - 12脂肪酸去饱和酶
基因( fad2)突变体对油菜叶片表面结构和透性的影
响[ J ]. 植物生理与分子生物学学报, 2003, 29 ( 3 ) :
192—198.
[ 20 ] 　KinneyA J, Cahoon E B, HitzW D ,Manipulation desat2
urase activities in transgenic crop p lants [ J ]. Biochem
Soc Trans, 2001, 30: 1099—1103.
[ 21 ] 　BrummellD A , Balint - Kurti P J , Harp sterM H , et
al. Invert repeat of a heterologous 3’- untranslated re2
gion for high - efficiency, high - throughput gene silen2
cing[ J ]. The Plant Journal, 2003, 33: 793—800.
[ 22 ] 　Chuang C,Meyerowitz E. Specific and heritable genetic
interference by double - stranded RNA in Arabidop sis
thialiana [ J ]. Proc Natl Acad Sci, 2000, 97: 4985—
4990.
[ 23 ] 　Schweizer P, Pokorny J , Schulez - Lefer P, et al. Double
- stranded RNA interfereswith gene function at the sin2
gle cell level in cereals [ J ]. Plant J , 2000, 24: 895—
903.
[ 24 ] 　WangM B,Waterhouse PM. App lication of gene silen2
cing in p lants [ J ]. Current Op inion in Plant Biology,
2001, 5: 146—150.
[ 25 ] 　Wterhouse PM, Helliwell C A. Exp loring p lant genomes
by RNA - induced gene silencing [ J ]. Nature Review
Genetics, 2003, 4: 29—38.
[ 26 ] 　Smith N A, Singh S P,WangM B, et al. Total silencing
by intron - sp liced hairp in RNAs [ J ]. Nature, 2000,
407: 319—320.
[ 27 ] 　Wesley S V, Helliwell C A, Smith N A, et al. Construct
design for efficient, effective and high - throughput gene
silencing in p lants[ J ]. Plant J , 2001, 27: 581—590.