Establishment of an in vitro embryonic growth system and its application in rapeseed oil synthesis and accumulation study

doi:10.19802/j.issn.1007-9084.2019192

Abstract

Abstract: To facilitate the study on embryo developrnent and fatty acid accumulation, tissue culture of rape
seeds were carried out by using Brassica napus spring type 862. Study of influence factors on 862 oil synthesis and
accumulation showed that B5 basal medium with 8% sucrose and 0.6% agar was optimal. Extracts from pericarp
and leaf of different varieties in the medium could improve oil accumulation efficiency in embryo. While pericarp
extract had higher accumulation efficiency than that from leaf. Extracts of pericarp and leaf from the high oil variet⁃
ies possessed much higher oil accumulation efficiency.

Key words: , Brassica napus L., embryo, in vitro tissue culture, oil content

CLC Number:

中图分类号：S565.4

DENG Lin-bin, FAN Shi-hang, SUN Xing-chao, HUA Wei, LIU Jing. Establishment of an in vitro embryonic growth system and its application in rapeseed oil synthesis and accumulation study[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(2): 203-.

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