CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (2): 203-.doi: 10.19802/j.issn.1007-9084.2019192

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Establishment of an in vitro embryonic growth system and its application in rapeseed oil synthesis and accumulation study


  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and
    Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
  • Online:2021-04-28 Published:2021-03-30

Abstract: To facilitate the study on embryo developrnent and fatty acid accumulation, tissue culture of rape
seeds were carried out by using Brassica napus spring type 862. Study of influence factors on 862 oil synthesis and
accumulation showed that B5 basal medium with 8% sucrose and 0.6% agar was optimal. Extracts from pericarp
and leaf of different varieties in the medium could improve oil accumulation efficiency in embryo. While pericarp
extract had higher accumulation efficiency than that from leaf. Extracts of pericarp and leaf from the high oil variet⁃
ies possessed much higher oil accumulation efficiency.

Key words:  , Brassica napus L., embryo, in vitro tissue culture, oil content

CLC Number: