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中国油料作物学报 ›› 2015, Vol. 37 ›› Issue (3): 269-.doi: 10.7505/j.issn.1007-9084.2015.03.003

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Inheritance of imidazolinone resistance in rapeseed 12WH318 and BnALS gene cloning

  

  1. 1.Oil Crop Research Institute of the Chinese Academy of Agricultural Science, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China;2.Hubei University,Wuhan,430062,China
  • Online:2015-06-28 Published:2015-09-07

Abstract:

 12WH318 is a novel rapeseed germplasm (Brassica napus) with imidazolinones resistance, which was screened and isolated from imported transgenic oilseed - rape. The inheritance and molecular mechanism of imidazolinones resistance in 12WH318 were studied for better utilization of 12WH318. Result showed that imidazolinones resistance from 12WH318 was controlled by one pair of genes, in mode of complete dominance without cytoplasmic effect. Imidazolinone herbicide targeted acetolactate synthase (ALS) genes (BnALS1, BnALS2 and BnALS3) which were isolated from 12WH318 and 3 susceptive rapeseed lines by homologous strategy. According to alignment of DNA and amino-acids sequences, BnALS1 gene from 12WH318 contained mutant loci, in which a serine (AGT) at the 638th base became asparagine acid (AAT). Relative expressions showed that both BnALS1 and BnALS3 had a burst in leaves after herbicide treatment. After 7 days, BnALS1 expression was significantly lower than that of water control, and BnALS3 expression was similar to that of water control. It was concluded that the imidazolinones resistance in 12WH318 was controlled by BnALS1 gene.

Key words: Brassica napus L., Imidazolinones, Acetolactate synthase (ALS), Herbicide resistance, Gene expression analysis