中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (3): 421-.doi: 10.7505/j.issn.1007-9084.2019.03.015

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Transcriptome analysis of Brassica napus-Plasmodiophora brassicae interaction during early infection


  1. 1.College of Plant Protection,Yunnan Agricultural University, Kunming Yunnan 650201,China; 
    2. Industrial Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming Yunnan 650205, China
  • Online:2019-06-28 Published:2019-09-02


Clubroot is one of the most serious disease in oilseed rape. To explore the resistant molecular mechanism of the interaction between Plasmodiophora brassicae and Brassica napus during early infection, gene differential expression analysis of the clubroot-resistant rapeseed line ZHE-226(R) and clubroot-susceptible line 10159 (S) were performed by RNA-seq. A total of 809 and 1082 genes were commonly up- and down-regulated at 0, 12, 48, 72h post-inoculation (hpi) time points in R line. Pattern recognition receptors (PRRs), chitinase, R gene, WRKY transcription factor, salicylic acid (SA) and jasmonic acid (JA) were involved in the early response to clubroot infection in lines of R and S, but showed different expression patterns. Compared to S line, most of the PRRs genes were down-regulated, R genes were up-regulated and SA genes were down-regulated in earlier stage but up-regulated at 72hpi in R line. Chitinase, WRKY and JA related genes were both induced in R and S lines. These results showed that PTI- mediated by PRRs was significantly induced in S line, but played a small role in R line. ETI-mediated by R gene and resistance signal pathway-mediated by SA played great role in clubroot resistance. This research provided a better understanding of the resistance genes of B. napus in response to P.brassicae, and assistance for clubroot-resistant breeding.

Key words: clubroot resistance, Plasmodiophora brassicae, Brassica napus, RNA-seq