中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (6): 858-.doi: 10.19802/j.issn.1007-9084.2019017

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Identification and codon bias of NBS-LRR gene family in Brassica napus


  1. 1. College of Life Sciences, North China University of Science and Technology, Tangshan 063210,China; 
    2. Depart ment of Biological Sciences, Tangshan Normal University, Tangshan 063000, China
  • Online:2019-12-28 Published:2019-12-20


NBS-LRR (nucleotide binding site and leucine rich repeat) is a large complex gene family playing important role in plant disease resistance. For better understanding of NBS-LRR gene family in Brassica napus (L.), bioinformatics analyses were conducted in B. napus and other 4 species (B. oleracea, B. rapa and Arabidopsis thali? ana and Oryza sativa). As a whole, family identification, phylogenetic analysis, conserved motif, gene structure, chromosome localization and codon bias were analyzed. Results showed that 463 NBS-LRR members were identi? fied in B. napus. Referenced by A. thaliana, the members were divided into 2 groups, TIR-NBS-LRR and CCNBS- LRR, which were further divided into 4 and 8 subgroups respectively. All 19 chromosomes contained NBSLRR genes. Among them, 53 genes distributed on ChrC09. The NBS-LRR genes were mostly presented in clusters. Large-scale fragment replication of the genes was speculated. Results of codon bias analysis of the family in the 5 species showed that ENC (effective number of codon) of B. napus genes was 37.76 to 61.00, which was similar to B. oleracea in their preference. Codon bias features by preference of collinear homologous relationship showed more similarity between B. napus and B. oleracea.

Key words: Brassica napus, NBS-LRR gene family, phylogenetic evolution, chromosome localization, synte?ny, codon bias