CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (5): 1006-1017.doi: 10.19802/j.issn.1007-9084.2021253


Cloning and genetic transformation of soybean fatty acid dehydrogenase GmFAD3C-1 gene

Su-qi JIAO(), Jun-ming ZHOU, Yu-qing SHANG, Jia-xin WANG, Ai-jing ZHANG, Hao-bo HE, Qiu-zhu ZHAO, Yue LI, Dan YAO()   

  1. College of Life Sciences, Jilin Agricultural University,Changchun 130118,China
  • Received:2021-10-05 Online:2022-10-25 Published:2022-10-31
  • Contact: Dan YAO E-mail:asu0106@163.com;dyao@jlau.edu.cn


By comparing the sequences of four key enzyme genes in the soybean fatty acid dehydrogenase GmFAD3 family, the GmFAD3C-1 gene had a base site deletion (G) at +966bp in the low linolenic acid soybean mutant MT72 compared with the control JN18, producing a frameshift mutation resulting in large changes in the amino acid sequence (the mutant gene is named gmfad3c-1). The GmFAD3C-1 gene overexpression and its CRISPR / Cas9 editing vectors were constructed, and the T2 transformed plants were obtained using the pollen tube channel method. The fatty acid dehydrogenase enzyme activity assay showed that in the T2 grain of overexpressing plants, enzyme activity increased 47.62%-78.85% compared to control, and in the editing vector T2 generation, enzyme activity decreased 25.67%-47.11% compared to control. The results of the fatty acid relative content determination further indicate that the expression of the GmFAD3C-1 gene is closely related with plant linolenic acid content.

Key words: soybean, linolenic acid, mutant, fatty acid dehydrogenase, GmFAD3C-1 gene

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