CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1275-1285.doi: 10.19802/j.issn.1007-9084.2021274
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Qi-fei ZHANG1(), Jian-miao CHEN1(
), Xin-xin HOU1, Shu-ya LI1, Jian WANG2, Hang-gui LAI1
Received:
2021-11-04
Online:
2022-12-25
Published:
2022-11-24
Contact:
Jian-miao CHEN
E-mail:zqifeizhang@163.com;cjm@hainanu.edu.cn
CLC Number:
Qi-fei ZHANG, Jian-miao CHEN, Xin-xin HOU, Shu-ya LI, Jian WANG, Hang-gui LAI. In vitro rapid propagation of Camellia vietnamensis Haida youcha 4 and nurse seed grafting using its tissue culture bud as scion[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(6): 1275-1285.
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URL: http://www.jouroilcrops.cn/EN/10.19802/j.issn.1007-9084.2021274
Table 2
Effects of different disinfection treatments on disinfection of inoculated explants
编号 Code | 消毒处理方式 Disinfection treatment | 污染率 /% Contamination rate | 诱导率 /% Induction rate | 芽萌发级别 Bud germination grade |
---|---|---|---|---|
① | 0.1% HgCl2 (12 min) | 96.7±2.3 a | 2.7±0.3 d | III、IV级为主 Mainly Grade III and IV |
② | 0.1%多菌灵摇床振荡4 h+0.1%HgCl2 12 min 0.1 % carbendazim on shaker (4 h) + 0.1 % HgCl2 (12 min) | 82.5±4.6 b | 16.7±1.7 c | II、III级为主 Mainly Grade II and III |
③ | 0.1% HgCl2 (12 min) + 2% NaClO (15 min) | 70.7±3.9 c | 20.5±1.5 c | I、II级为主 Mainly Grade I and II |
④ | 0.1%多菌灵摇床振荡4 h+0.1% HgCl2 12 min+2% NaClO 15 min 0.1% carbendazim on shaker (4 h) + 0.1% HgCl2 (12 min) + 2% NaClO (15 min) | 67.5±5.1 c | 10.7±1.9 d | II、III级为主 Mainly Grade II and III |
⑤ | 0.1% HgCl2 12 min + 2% NaClO 15 min + 0.1 g/L清菌易 0.1% HgCl2 12 min + 2% NaClO 15 min + 0.1 g/L germicide (Qingjunyi) | 16.7±1.9 d | 61.5±2.6 a | I、II级为主 Mainly Grade I and II |
⑥ | 0.1% HgCl2 12 min + 2% NaClO 15 min + 0.5 mL/L植菌清 0.1% HgCl2 12 min + 2% NaClO 15 min + 0.5mL/L germicide (Zhijunqing) | 65.4±16.5c | 32.3±1.6 b | II、III级为主 Mainly Grade II and III |
Table 3
Effects of different cytokinins on the induction of apical bud and axillary bud primaries
外植体 Explant | 细胞分裂素 Cytokinin /(mg/L) | K1 /% | K2 /% | K3 /% |
---|---|---|---|---|
顶芽 Terminal bud | 0 | 63.3±2.6 bc | 29.3±4.2 b | 4.7±1.3 c |
6-BA | 69.7±6.9bc | 43.7±3.1 a | 49.1±6.3 a | |
KT | 61.9±3.2 c | 30.1±5.3 b | 43.2±3.1 ab | |
TDZ | 65.6±1.4 bc | 35.1±4.9 ab | 44.6±2.5 ab | |
腋芽 Axillary bud | 0 | 75.2±5.7 b | 30.7±2.1 b | 4.3±0.7 c |
6-BA | 83.9±6.1 a | 42.9±4.9 a | 45.6±4.9 ab | |
KT | 72.4±4.3 bc | 38.5±4.6 ab | 36.9±2.7 b | |
TDZ | 76.1±1.7 b | 37.9±6.3 ab | 44.3±3.2 ab |
Fig. 1
Induction of terminal buds and axillary buds by different cytokininsNote: A: Inoculated axillary and terminal buds explants with 1/3 leaves left; B: Sterile buds were induced by terminal bud and axillary buds; C: Sterile buds were induced by different cytokinins (from left to right: without any cytokinin, 6-BA added, KT added, TDZ added)
Table 4
Effects of different basic media and exogenous hormone on bud regeneration
序号 Serial number | 培养基 Sulture medium | 6-BA mg/L | IAA mg/L | GA3 mg/L | 增殖系数 Growth coefficient | 芽生长情况 Bud growth |
---|---|---|---|---|---|---|
A1 | MS(b) | 1.0(b) | 0.05(a) | 0.5(c) | 4.3±0.9 b | 正常,叶绿色、少量叶片卷曲,茎节短 Normal, leaf green, some leaf curled, stem node short |
A2 | MS(b) | 2.0(a) | 0.1(b) | 1.0(b) | 4.6±0.5 ab | 正常,叶绿色正常、茎节短 Normal, green leaves normal, stem nodes short |
A3 | MS(b) | 3.0(a) | 0.5(c) | 2.0(a) | 4.0±0.5 b | 正常,叶绿色叶片较大,茎节短 Normal, green leaves larger, internodes shorter |
A4 | B5(c) | 1.0(b) | 0.1(b) | 2.0(a) | 3.3±0.4 c | 芽细长淡绿,茎节长 Buds slender, light green, internodes long |
A5 | B5(c) | 2.0(a) | 0.5(c) | 0.5(c) | 3.1±0.4 c | 芽细弱,茎节短,部分叶片后期萎蔫 Buds slender, internodes short, some leaves wilt later |
A6 | B5(c) | 3.0(a) | 0.05(a) | 1.0(b) | 4.2±0.3 bc | 芽细长淡绿,部分基部长愈伤 Buds slender, light green, part of base minister callus |
A7 | WPM(a) | 1.0(b) | 0.5(c) | 1.0(b) | 4.8±0.5 ab | 芽正常,叶墨绿、茎节长 Bud normal, leaf dark green, stem node long |
A8 | WPM(a) | 2.0(a) | 0.05(a) | 2.0(a) | 5.7±0.3 a | 芽健壮墨绿,茎节长 Bud strong dark green, stem node long |
A9 | WPM(a) | 3.0(a) | 0.1(b) | 0.5(c) | 5.0±0.3 ab | 芽健壮墨绿,茎节短 Buds strong dark green, internodes short |
Table 5
Strong bud culture of regenerating buds in tissue culture
序号 Serial number | 芽茎粗 /cm Stem diameter | 芽长 /cm Bud length | 芽生长情况 Growth status |
---|---|---|---|
S1 | 0.23±0.02 c | 3.31±0.11 c | 基部有新生愈伤和小芽,多芽相对细小,小叶绿色 New callus and buds at base, many buds relatively small, leaflets green |
S2 | 0.32±0.05 b | 4.26±0.23 b | 单芽生长正常,叶绿色,舒展 Single bud growth normal, leaf green, stretch |
S3 | 0.33±0.01 ab | 5.35±0.09 a | 基部有新生愈伤和小芽,芽生长正常,叶绿色,舒展 New callus and bud at base, bud growth normal, leaf green, spreading |
S4 | 0.38±0.01 a | 5.36±0.25 a | 单芽生长健壮,叶绿色,舒展 Single bud robust, leaf green, stretch |
S5 | 0.36±0.02 ab | 5.28±0.06 a | 单芽生长健壮,叶墨绿色,舒展 Single bud grows robust, leaves dark green, stretch |
Table 6
Effect of different scion treatment on seedling rate of nurse seed grafting
接穗类型 Scion type | 接穗处理 Scion processing | 成苗率 /% Seedling rate | 新生叶片数 Young leaves | 嫁接接口愈合及嫁接小苗长势 Grafting interface healing and grafting seedling growth |
---|---|---|---|---|
一叶一芽 One-leaf-one-bud | 清水 Water | 81.0±2.7 cd | 4.7±0.58 a | 嫁接口愈合正常、接口平整,叶绿色,苗长势正常 Grafting port healed normally, interface smooth, leaf green, seedlings grow normally |
组培芽 Tissue cultured bud | 清水 Water | 80.0±2.0 d | 3.3±0.58 cd | 嫁接口愈合相对慢,接口平整,叶绿色,苗长势正常 Grafting part healed slow, interface smooth, leaf green, seedling grow normally |
2,4-D | 85.3±1.5 bc | 3.0±0.0 cd | 嫁接口愈合快,接口处愈伤组织多,叶绿色,苗长势正常 Grafting interface heals quickly with calluse, leaf green, seedling grow normally | |
6-BA | 91.3±1.5 a | 4.3±0.58 ab | 嫁接口愈合快,接口处平整,叶墨绿色,苗生长旺 Grafting part heals quickly with smooth interface, leaf dark green, seedling grows vigorously | |
NAA | 86.7±2.1 ab | 2.7±0.58 d | 嫁接口愈合较快,接口处平整,叶墨绿色,苗长势正常 Grafting part heals quickly with smooth interface, leaf dark green, seedlings grow normally | |
TDZ | 89.7±3.2 ab | 3.7±0.58 bc | 嫁接口愈合快,接口处有少许愈伤组织圈,叶墨绿色,苗生长旺 Grafting part heals quickly with a few callus circles at the interface, leaf dark green, seedling grows vigorously |
Table 7
Growth of nurse seed grafting of different kinds of scion
接穗类型 Scion types | 60 d | 85 d | |||
---|---|---|---|---|---|
新梢长相对增长量 /% Relative growth of shoot length | 新梢茎粗相对增长量 /% Relative increase in stem diameter of new shoots | 新梢长相对增长量 /% Relative growth of shoot length | 新梢茎粗相对增长量 /% Relative increase in stem diameter of new shoots | ||
一叶一芽 One leaf and one bud | 70.5±4.1 a | 34.1±3.3 a | 78.8±4.1 a | 24.4±3.3 b | |
无菌单芽 Sterile single bud | 48.8±5.4 b | 37.3±2.9 a | 71.3±5.4 a | 66.2±5.9 a |
Fig. 4
Germinated seed grafting with tissue culture buds as scionsNote: A: Tissue culture bud scion; B: Tissue culture bud scion bud rootstock grafting; C: 60 d tissue culture bud scion bud stock grafting seedling; D: 85 d tissue culture sprouting rootstock grafting seedlings; E: One-leaf-one-bud scion; F: Grafting one-leaf-one-bud stock; G: 60 d one-leaf-one-bud stock grafting; H: 85 d one-leaf-one-bud scion rootstock grafting seedling
1 | 庄瑞林. 中国油茶[M]. 2版. 北京: 中国林业出版社, 2008. |
2 |
Guo L X, Xu X M, Yuan J P, et al. Characterization and authentication of significant Chinese edible oilseed oils by stable carbon isotope analysis[J]. J Am Oil Chem Soc, 2010, 87(8): 839-848. DOI:10.1007/s11746-010-1564-3 .
doi: 10.1007/s11746-010-1564-3 |
3 | 周亚东. 浅谈油茶文化[J]. 热带林业, 2017, 45(2): 42-48. |
4 | 陈有师, 薛杨. 油茶芽苗砧嫁接育苗技术[J]. 现代农业科技, 2019(16): 47-48. |
5 |
吴习安, 陈凤翔, 彭新春. 油茶芽苗砧嫁接试验[J]. 湖南林业科技, 2010, 37(5): 50-52. DOI:10.3969/j.issn.1003-5710.2010.05.015 .
doi: 10.3969/j.issn.1003-5710.2010.05.015 |
6 | 王斌, 王开良, 童杰洁, 等. 我国油茶产业现状及发展对策[J]. 林业科技开发, 2011, 25(2): 11-15. |
7 | 侯辛辛. 海南白花油茶离体快速繁殖和再生体系的建立及应用[D]. 海口: 海南大学, 2020. |
8 | 陈晓玲. 茶树离体再生体系与微型嫁接的研究[D]. 合肥: 安徽农业大学, 2011. |
9 |
蔡玲, 王以红, 吴幼媚, 等. 油茶胚组织离体培养实验[J]. 西部林业科学, 2012, 41(3): 66-69. DOI:10.16473/j.cnki.xblykx1972.2012.03.022 .
doi: 10.16473/j.cnki.xblykx1972.2012.03.022 |
10 |
王瑞, 陈永忠, 王湘南, 等. 油茶组培苗高效增殖体系的建立[J]. 中南林业科技大学学报, 2015, 35(10): 40-43. DOI:10.14067/j.cnki.1673-923x.2015.10.007 .
doi: 10.14067/j.cnki.1673-923x.2015.10.007 |
11 |
毕方铖, 谭晓风, 张智俊, 等. 油茶离体培养诱导再生植株的研究[J]. 经济林研究, 2004, 22(2): 5-9. DOI:10.3969/j.issn.1003-8981.2004.02.002 .
doi: 10.3969/j.issn.1003-8981.2004.02.002 |
12 |
吴茂力, 敬袁兵, 张恺凯, 等. 生长调节剂对油茶离体诱导及增殖的影响[J]. 耕作与栽培, 2016(6): 6-7, 10. DOI:10.13605/j.cnki.52-1065/s.2016.06.002 .
doi: 10.13605/j.cnki.52-1065/s.2016.06.002 |
13 |
李泽, 谭晓风, 袁军, 等. 油茶良种‘华硕’的组织培养及高效生根[J]. 植物生理学报, 2014, 50(11): 1721-1726. DOI:10.13592/j.cnki.ppj.2014.0369 .
doi: 10.13592/j.cnki.ppj.2014.0369 |
14 | 贾效成, 陈良秋, 刘小玉, 等. 海南本地油茶组培体系的建立[J]. 现代农业科技, 2018(12): 1, 5. |
15 |
周广振, 栾林莉, 宋玉凤, 等. 海南白花油茶花药再生体系的初步建立[J]. 经济林研究, 2018, 36(1): 49-56. DOI:10.14067/j.cnki.1003-8981.2018.01.008 .
doi: 10.14067/j.cnki.1003-8981.2018.01.008 |
16 |
邓丽峰, 张军锋, 吴友根, 等. 海南油茶内生真菌抗氧化活性测定及化学成分分析[J]. 分子植物育种, 2019, 17(3): 995-1005. DOI:10.13271/j.mpb.017.000995 .
doi: 10.13271/j.mpb.017.000995 |
17 | 龚峥, 王洪峰, 张弘, 等. 广宁红花油茶组织培养育苗技术研究[J]. 广东林业科技, 2015, 31(2): 7-14. |
18 | 万珠珠, 谭秀梅, 牛来春, 等. 不同处理对油茶继代增殖培养的影响[J]. 中国园艺文摘, 2016, 32(4): 31-32. |
19 | 刘海龙, 陈晓明, 蔡玲, 等. 油茶不同外植体灭菌条件研究[J]. 广西林业科学, 2010, 39(2): 61-63, 68. |
20 | 孟路. 4个楸树良种离体繁殖能力及组培技术研究[D]. 北京: 北京林业大学, 2019. |
21 | 王成韬, 钟秋平, 李永成. 海南粗榧外植体灭菌方法研究[J]. 热带作物学报, 2014, 35(1): 110-114. |
22 |
朱广廉. 植物组织培养中的外植体灭菌[J]. 植物生理学通讯, 1996, 32(6): 444-446. DOI:10.13592/j.cnki.ppj.1996.06.020 .
doi: 10.13592/j.cnki.ppj.1996.06.020 |
23 |
张雪冰, 王鸿, 张帆, 等. 山桃外植体的安全消毒方法研究[J]. 甘肃农业科技, 2019(6): 29-32. DOI:10.3969/j.issn.1001-1463.2019.06.008 .
doi: 10.3969/j.issn.1001-1463.2019.06.008 |
24 | 刘海英. 油茶组培再生体系建立及愈伤组织诱导[D]. 武汉: 华中农业大学, 2011. |
25 |
黄莉雅, 张日清, 马锦林, 等. 不同培养条件对油茶愈伤组织和芽诱导的影响[J]. 安徽农业科学, 2010, 38(4): 1718-1719, 1740. DOI:10.13989/j.cnki.0517-6611.2010.04.146 .
doi: 10.13989/j.cnki.0517-6611.2010.04.146 |
26 | 张法勇, 刘向东, 高秀丽. 木本植物组织培养器官发生植株再生研究进展[J]. 河北林果研究, 2005, 20(3): 234-238. |
27 |
陈继富. 无籽罗汉果的组织培养和快速繁殖[J]. 植物生理学报, 2013, 49(9): 968-972. DOI:10.13592/j.cnki.ppj.2013.09.011 .
doi: 10.13592/j.cnki.ppj.2013.09.011 |
28 |
高洁, 张萍, 薛璟祺, 等. 酚类物质及其对木本植物组织培养褐变影响的研究进展[J]. 园艺学报, 2019, 46(9): 1645-1654. DOI:10.16420/j.issn.0513-353x.2018-0698 .
doi: 10.16420/j.issn.0513-353x.2018-0698 |
29 | 张卫建. 不同处理对油茶芽苗砧嫁接苗成活率和生长量的影响[J]. 安徽林业科技, 2012, 38(3): 26-29. |
30 | 胡观兴. 海南油茶芽苗砧嫁接与容器育苗技术研究[D]. 长沙: 中南林业科技大学, 2020. |
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