CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1275-1285.doi: 10.19802/j.issn.1007-9084.2021274

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In vitro rapid propagation of Camellia vietnamensis Haida youcha 4 and nurse seed grafting using its tissue culture bud as scion

Qi-fei ZHANG1(), Jian-miao CHEN1(), Xin-xin HOU1, Shu-ya LI1, Jian WANG2, Hang-gui LAI1   

  1. 1.College of Tropical Crops, Hainan University, Haikou 570228, China
    2.College of Horticulture, Hainan University, Haikou 570228, China
  • Received:2021-11-04 Online:2022-12-25 Published:2022-11-24
  • Contact: Jian-miao CHEN E-mail:zqifeizhang@163.com;cjm@hainanu.edu.cn


To establish an efficient in vitro propagation system for Camellia oleifera varieties in Hainan Province, as well as to speed up the propagation procedures for rootstock grafted seedlings, axillary and apical buds of the current-year branches from C. vietnamensis cv Haidayoucha 4, were used as explants. Initial generation was used to induce germination of fixed buds for establishing a sterile system. Then, buds regeneration coefficient was improved for buds propagation. Later, the regenerative buds were further strengthened and refined, as scions for C. oleifera germinated seed grafting. Results showed that the most suitable disinfection method for explants was a combined disinfection with 2% NaClO for 15 min and 0.1% HgCl2 for 12 min, and the explants were cultured on the medium with 0.1 g/L Qingjunyi (a microbicide). This method reduced the contamination rate to below 20%. The suitable medium for primary bud induction of axillary- and apical-bud was MS + 0.1 mg/L IAA + 2 mg/L 6-BA + 0.5 mg/L GA3. The suitable medium for regeneration of aseptic tissue culture buds was WPM + 2 mg/L 6-BA + 0.05 mg/L IAA + 2 mg/L GA3. The suitable medium for tissue culture of single bud was WPM + activated carbon 500 mg/L. When the bud scions were soaked in 6-BA 50 mg/L for 2 min, the grafting seedling rate could reach more than 90%.

Key words: Camellia vietnamensis Haida youcha 4, rapid propagation in vitro, tissue culture bud scion, germinated seed grafting

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