Both NFR5 and NFR1 are involved in signal perception and transduction of Nod factor produced by rhizobia in Lotus japonicus. In this research, using yeast two hybrid approach , we constructed AD-cDNA library of L. japonicus and idendified 221 positive clones using the protein kinase domain of NFR5(nfr5-pk) as a bait at pre-screening. After retranformed and X-Gal assay, 26 positive clones were confirmed. Six kinds of proteins including small G-Protein Rop6 were obtained after sequencing and NCBI blast analysis. These proteins could be participated in the regulating function of NFR5.
one new CMS source CMS-514 and its maintainer were derived from the embryo resourced after the cross was made between H.tuberosus and the maintainer 7718B line 4 days. The number of chromosome of the CMS-514 and the maintainer were 2n=34. the new CMS source and the maintainer were studied for its utilization in breeding. Sixty lines including CMS, maintainer and restorers relating to the CMS-PET-1 and others CMS source. Ten crosses were made between ten CMS-PET-1 and the maintainer of CMS-514A, the hybridization plants all have pollen in F1 progenies, the plants showed segregation in F2 progenies when they flowering; 30 crosses were made between the CMS-514A and the maintainer and restorer of the CMS-PET-1. The hybridization plants all have no pollen; 20 crosses were made between the CMS-514A and other materials at Northern Crop Science Laboratory USD A-ARS, the hybridization plants all have no pollen. The CMS-514A and its maintainer are new CMS source and not line the CMS-PET-1, CMS-CMG1, CMS-CMG2, CMS-CMG3 and some other CMS sources. The trait of mail sterility controlled by one gene and keeping the gegetic system od cytoplasmic mail-sterile and genetic fertility restoration.