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    遗传育种
  • 遗传育种
    ZHANG Jie-fu
    2007, 29(4): 359-364.
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    A low erucic acid content line APL01 was crossed with a high erucic acid content variety M083, and six basic generations (P1, P2, F1, B1, B2 and F2) derived from this cross were used to analyze the genetic patterns of fatty acids by applying major gene and polygene mixed model. Results showed that the contents of palmitic and eicosenoic acids were controlled by two additive-dominance-epistasis major genes and additive-dominance polygene, and the additive effect of major genes for palmitic acid was larger than dominance effect, while the additive and dominace effects of major genes controlling eicosenoic acid were all larger. The contents of stearic, oleic, linoleic and linolenic acids were all controlled by two additive-dominance-epistasis major genes and additive-dominance-epistasis polygenes. The additive effect of major genes for stearic acid was larger, and the heritability of major genes was 75.00%-92.45%. The additive effects of two major genes for oleic acid were 14.38 and 9.92, the dominance effects were -2.24 and -0.44, respectively. The heritability of two major genes was large, being 81.93%-92.68%. The additive effect of major genes for linoleic acid was larger than dominance effect, the same as linolenic acid. Erucic acid was controlled by two additive –dominance major genes. The additive effects of these two major genes were -12.27 and -8.83, the dominance effects 0.35 and 1.69, respectively. No epistasis and polygene effects existed in erucic acid inheritance, and the heritability of major gene was 92.54%- 96.72%.
  • 遗传育种
    WEI Wen-hui
    2007, 29(4): 365-371.
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    The 36 day embryoes of high oil content variety “Zhongdou 32”and low oil content variety “Youchun 02-6” were used to construct suppression subtractive cDNA library by suppression subtractive hybridization. The inserted fragment sizes focus on 500 bp, and 766 clones were verified by PCR, among which 700 clones were used to perform dot blot hybridization. After dot blot hybridization, 575 clones were obtained and sequenced. 237 differentially expressed genes were obtained by homologous BLAST search. Besides 10 unknown function genes, others related to protein storing, protein degradation, auxin regulation and so on. Parts of differentially expressed genes probably relating to oil synthesis were detected by RT-PCR and Real-time quantitative PCR, their functions were also primarily discussed, which will be a guidance for studying the functions of these genes during oil synthesis.

  • 遗传育种
    CHEN Chun-fen, ZHU Hui, YANG Qi, ZHU Hao, ZHANG Zhong-ming*
    2007, 29(4): 372-376.
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    Both NFR5 and NFR1 are involved in signal perception and transduction of Nod factor produced by rhizobia in Lotus japonicus. In this research, using yeast two hybrid approach , we constructed AD-cDNA library of L. japonicus and idendified 221 positive clones using the protein kinase domain of NFR5(nfr5-pk) as a bait at pre-screening. After retranformed and X-Gal assay, 26 positive clones were confirmed. Six kinds of proteins including small G-Protein Rop6 were obtained after sequencing and NCBI blast analysis. These proteins could be participated in the regulating function of NFR5.

  • 遗传育种
    LI Yun-chang, HU Qiong*, MEI De-sheng, LI Ying-de, XU Yu-song, Li Xiao-qing, Yu You-qiao Tan Zu-meng
    2007, 29(4): 377-381.
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    The yield and quality of rapeseed varieties have been improved significantly after breeding practice over more than twenty years in China. Compared to those varieties developed in industrialized countries, however, the yield of Chinese rapeseed varieties is still low. Further improvement of rapeseed yield relies on the development of elite parental lines and the full exploitation of heterosis for hybrid production. This study made use of germplasm with traits complementary to each other with different genetic background, to breed the maintainers and restorers of Shaan 2A cytoplasmic male sterility first, then to predict the heterosis by the evaluation of genetic distance between parental lines. Based on the heterosis prediction, a hybrid variety Zhongyouza 12 with high yield and good quality was successfully developed. The yield of Zhongyouza 12 not only surpassed Zhongshuang 9, which is a largely extended open pollinated variety in Hubei province, by 15.03%, but also over yielded Zhongyouza 2, which has been the highest yielding hybrid along Yangtze river basin, by 5.62%, becoming the first rapeseed variety which over yielded Zhongyouza 2 in national trials and with yield over 3000 kg.ha-1 in Hubei provincial trials. Due to the stable yield and high oil content, the oil yield of Zhongyouza 12 was stabilized above 1100 kg.ha-1 in national trials, increase by 9.06% compared to Zhongyouza 2. The effects of multiple crosses on the elevation of maintaining ability of cytoplasmic male sterility and the realization of yield potential are also discussed.
  • 遗传育种
    WANG Gui-chun,LIU Zhi,YANG Guang-sheng*
    2007, 29(4): 382-386.
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    The effects of genotype,cultural density and inflorescence stage on embryo production from microspores of three high oil content lines were investigated. The results showed that there were significant genotypic differences for embryogenesis among these genotypes. But all the appropriate density of microspore culture of this three materials was 3 buds/ utensil .The highest embryogenesis frequency was obtained from buds sampled at initial efflorescence afer one week. Analysis of oil content by the NIRS (near-infrared reflectance spectroscopy) showed that microspore culture was an effective approach for selecting high oil content DH line. Among 40 DH lines derived from 1481 and 1489, oil content of two DH lines, WD-33 and WD-34, reach above 47%.
  • 遗传育种
    Li Peiwu , DING Xiao-xia, ZHANG Wen, ZHAO Yong-guo,
    LI Yun-chang, XIE Cong-hua,Fu Tingdong
    2007, 29(4): 387-390.
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    The heterosis of glucosinolates in leaves of two rapeseed crosses were studied in this paper. Leaf glucosinolates content and components were analyzed by high performance liquid chromatography (HPLC). Both crosses presented mainly nagtive heterosis over both parents. The heterosis of glucosinolates in hybrids leaf were significant and negative in stem elongation and floral period, suggesting that it is difficult to increase leaf glucosinolates in these periods for high disease resistance through hybrid rapeseed and it would be the best time for harvesting the shoots as vegetable. All aliphatic glucosinolates presented nagtive heterosis over both parents. However, heterosis of aromatic and indolyl glucosinolates was different between crosses. Leaf glucosinolate contents and profiles varied significantly in double-high and double-low rapeseeds although aliphatic glucosinolates were the dominant ones in both leaves.

  • 遗传育种
    LI Zhi-guo LI Xi-huan ZHANG Cai-ying* MA Zhi-ying
    2007, 29(4): 391-396.
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    Genetic diversity of 40 vegetable soybean varieties was evaluated based on agronomic characters and AFLPs. The results showed that the 40 varieties could be divided into 3 groups according to the agronomic traits and AFLP polymorphic loci, respectively. Thirty DNA polymorphic bands were scored from amplification of 8 AFLP primer combinations, with 3.75 markers for each primer combination in average. And the range of pair similarity coefficient was from 0.3704 to 0.9998; the average pair similarity coefficient was from 0.5980 to 0.7924 with the total average of 0.7094. The results also showed a narrow genetic diversity among the vegetable soybean varieties tested, thus, it is necessary to expand the germplasm resource of vegetable soybean.
  • 遗传育种
    TIAN Wei-hua, XU Ke-zhang*, BING Xin, ZHAI Jun-feng, ,
    ZHANG Zhi-an1, CHENG Zhan-yu1, WU Zhi-hai1,
    2007, 29(4): 397-401.
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    In order to investigate changes of yield, harvest index, biomass, seeds fat content and seeds protein content , The sixteen soybean cultivars planted popularly under field trial were studied in Jilin province. The result indicated that biomass of soybean cultivars increased to a maximum around R6 and then declined. Biomass of old cultivars was faster for the modern cultivars so that by harvest maturity the modern cultivars had a significantly greater biomass than the old cultivars. Biomass, seed yield and harvest index increased with year of release. The annual yield improvement per hectare was 14.90kg ha-1.This value corresponds to a 1.04% increase in yield per year. And harvest index increased 0.09% per year. The fat content levels have been increased (0.87%) accompanied by a reduce (1.52%) in seed protein content with year of release. The increase in seed yield with year of release was significantly correlated with an increase in HI and biomass, However, positive correlation with seeds fat content and negative correlation with seeds protein content ,were not unobvious. The results of this study show genetic improvement of soybean yield had led to biomass and harvest index increase. Biomass and HI are considered as two important indexes to improve soybean yield.
  • 遗传育种
    CHEN Ben-Yin,JIANG Hui-Fang, LIAO Bo-Shou, REN Xiao-Ping
    2007, 29(4): 402-408.
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    Arachis species is important resource for cultivated peanut improvement because of its resistance to many diseases. Since 1979, some accessions of wild related species of peanut were introduced and conserved at the Oil Crops Research Institute of Chinese Academy of Agricultural Science. It is necessary to investigate the important characters including morphological traits and DNA molecular characters for germplasm conservation. In this paper, 22 botanical traits including stem thickness, height of main stem, length of branches, total numbers of branches, numbers of branches with pods, etc of 19 accessions of 9 species belonging to sections Arachis, Procumbentes and Extranervosae were investigated. The DNA molecular profile of these accession were analysed through SSR. The results indicated that 19 genotypes could be distinguished by 22 phenotypic traits, and different accessions had different phenotypic traits. A. rigonii of section Procumbentes, A. batizocoi and A. duranensis of section Arachis were distinguished from other accessions in most botanical traits. The SSR data results also showed that different accessions had different fingerprints. The results based on the botanical traits were similar to that based on SSR analysis. The primer 10D4 could detect 11 accessions of the 19 genotypes. A fingerprint of 19 genotypes in genus Arachis was constructed via the SSR markers.

  • 遗传育种
    ZHANG Xiao-Qian, SHAN Lei* ,TANG Gui-Ying, TENG Na, BI Yu-Ping*
    2007, 29(4): 409-415.
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    Using hypocotyl of matured peanut seeds as recipients, the inverted repeat sequence of partial Δ12 fatty acid desaturase gene AhFAD2 with strong seed specific soybean Lectin promoter and CaMV35S promoter, was introduced respectively into Arachis hypogaea L. via A grobecterium infection. Transgenic peanuts containing the inverted repeat structure were confirmed by PCR.

  • 遗传育种
    WANG De-xing, CUI Liang-ji,C.C.JAN
    2007, 29(4): 416-419.
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    one new CMS source CMS-514 and its maintainer were derived from the embryo resourced after the cross was made between H.tuberosus and the maintainer 7718B line 4 days. The number of chromosome of the CMS-514 and the maintainer were 2n=34. the new CMS source and the maintainer were studied for its utilization in breeding. Sixty lines including CMS, maintainer and restorers relating to the CMS-PET-1 and others CMS source. Ten crosses were made between ten CMS-PET-1 and the maintainer of CMS-514A, the hybridization plants all have pollen in F1 progenies, the plants showed segregation in F2 progenies when they flowering; 30 crosses were made between the CMS-514A and the maintainer and restorer of the CMS-PET-1. The hybridization plants all have no pollen; 20 crosses were made between the CMS-514A and other materials at Northern Crop Science Laboratory USD A-ARS, the hybridization plants all have no pollen. The CMS-514A and its maintainer are new CMS source and not line the CMS-PET-1, CMS-CMG1, CMS-CMG2, CMS-CMG3 and some other CMS sources. The trait of mail sterility controlled by one gene and keeping the gegetic system od cytoplasmic mail-sterile and genetic fertility restoration.

  • 遗传育种

    CUI Xiang-hua1 Huo-Lei2 DUAN Ying1 Zhao Jinhuan1 kang Jianmin3 Wang Jing4

    2007, 29(4): 420-424.
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    Multi-factor comprehensive evaluating analysia sesame variety of national district test in 2005 by application of the grey system theory and the fuzzy mathematics, the results showed the result with actual performance in regional test is identical , with the evaluation index 0.7095 of Zhu 03J-3 the best, to the evaluation index 0.6753 of 00-51013 next . 02-2319 evaluation index 0.1902 the worst,This result is degreed with the fact expression of the test field.

  • YANG Chun-jie, ZHANG Xue-kun, ZOU Chong-shun, CHENG Yong, ZHENG Pu-ying, LI Gui-ying
    2007, 29(4): 425-430.
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    The effects of drought stress simulated by 10% polyethylene glycol 6000 (PEG-6000) on physiological and biochemical traits of 14 rapeseed varieties (Brassica napus L.) were studied during germination stage. The results showed that relative vigor index of seedling varied between 0.32-0.79, and relative vigor index of 14 varieties was 0.49 on average. Drought stress affected significantly (P<0.01) seedling height, fresh weight and survival rate. Compared with control (water), 10% PEG-6000 treatment decreased seedling height by 40.68%, fresh weight by 34.2% and survival rate by 18% on average. Also, malomdiadehyde (MDA), soluble sugar, proline, soluble protein content, and electrical conductivity of rapeseed seedling increased significantly. Compared with control (water), 10% PEG-6000 treatment increased the content of MDA by 96.7%, soluble sugar by 75.0%, proline by 2204.9%, soluble protein by 56.2% and electrical conductivity by 34.8% on average. But there were not significant differences between PEG treatment and control on numbers of adventitious roots and length of taproot. The results indicated that relative vigor index could be used as the main index of drought tolerance in rapeseed during germination stage.
  • 栽培生理
  • 栽培生理
    WANG Hong-ling, CHEN Shao-guang, XIANG Shi-peng, CHEN Chen, HAO Xiao-yan, MA Hao
    2007, 29(4): 431-437.
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    Twenty spring and summer soybean cultivars were used to explore the effects of climate factors during the whole growth period, the vegetative and reproductive growth periods on the relative contents of their major storage protein fractions and subunits. The cultivars were grown at six sowing times, and the climate factors included the accumulation temperature (AT), the total sunshine duration (SD), the total rainfall (TR), the range of temperature (RT) and the average relative humidity (RH). The results showed that: (1)The total seed protein contents, the relative contents of major seed protein fractions and subunits of soybeans would change along with the different sowing times. (2)During the whole growth period, the effects of the five climate factors on the total protein contents of the twenty varieties, and spring varieties were significant difference at 0.01 or 0.05 level. But for summer cultivars, only the effects of the accumulation temperature, the total sunshine duration, and the total rainfall on the total protein contents were found to be significant. (3)During the whole growth period, the correlative coefficients among the accumulation temperature and the total sunshine duration, and the relativecontent of 11S and 11S/7S ratio of the twenty soybeans were very markedly negative, and the one between the accumulation temperature and the 7S relative content was positive at 0.05 level. However, no effects of the climate factors on the 7S and 11S relative contents and 11S/7S ratio of the spring and summer soybean, respectively, were found to be significant. (4)During the whole growth period, the diversities among the effects of the five climate factors on the relative contents of major storage protein subunits of twenty varieties were found; For the summer cultivars during the vegetative and reproductive growth periods, no effects of the five climate factors on the relative contents of the protein subunits were found significantly. However, the effects of five climate factors on many protein subunits of spring varieties were found to be significant at 0.05 or 0.01 level.

  • 栽培生理

    ZHANG Yi1, 2, 3, LIU Peng1, SHI Feng 2, CHEN Runxing4, ZHU Shuying1

    2007, 29(4): 438-442.
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    A potted experiment was conducted with two soybean (Glycine max(L.)Merrill ) varieties (1601 and Zhechun No.3) and three treatments. The leaves were used to determine the photosynthetic characteristics and observe the ultrastructural at full podding period. The results showed that high concentration of Fe2+ (300 mg/kg, 500 mg/kg) inhibited the photosynthetic activity, the leaf chlorophyll content, net photosynthetic , transpiration rate and water utilization efficiency decreased ,respectively. Ultrastructural observation showed that the ultrastructural damage were much more serious with the increase of Fe2+. This resulted in swelling of chloroplast, disruption of chloroplast membrane, some huge starch grains, lipid in the chloroplast, the granum thylakoids piled up loosely and the arrangement of it were disordered.
    As a result, excessive Fe2+ not only destroy physiological activities, but also cause damage to its ultrastructure. In addition, two test soybean varieties have definite genetic different in response to Fe2+ toxicity, and “Zhechun No.3” soybean is more sensitive to Fe2+ toxicity than “1601”.

  • 栽培生理
    L IU Hong - yan, ZHAO Ying - zhong

    2007, 29(4): 443-447.
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    In this study, we used 110 sesame cultivars and 5 wild collections as materials to investigate the differences of leaf chlorophyll content (CC) at early bloom (EB), medium bloom (MB) and full bloom (FB) stages, and its correlation with yield traits. The result showed that, variation of CC is small within cultivars while much larger among cultivars; comparing with cultivars, CC in wild collections is significantly higher; during development, CC increase significantly from EB to MB, and from MB to FB, but still keep a very high correlation; CC is significantly correlated with plant height, capsule axis length and 1000-seed weight, while only moderately correlated with yield per plant (YPP). By dividing the cultivars into high, medium and low CC groups, we found that YPP of high CC group are significantly higher than medium group, and higher than low group. Thus, it seams to be a feasible approach to increase seed yield by improving CC in sesame.
  • 土肥植保
  • 土肥植保
    SUN Jia-gang, ZUO Qing-song, SHI Jian-fei, LENG Suo-hu, DONG Zhao-di, SUN Tong-qing
    2007, 29(4): 448-451.
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    Study of Potassium Accumulation in seeds of rapeseed
    SUN Jia-gang, ZUO Qing-song, SHI Jian-fei, LENG Suo-hu, DONG Zhao-di, SUN Tong-qing
    (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,
    Yangzhou University,Yangzhou 225009,China)
    Abstract: The trials were carried out in 2005~2006 to investigate the K accumulation of seed through measuring the K content in seed of rapeseed cultivar “Yangyou No. 6” (Brassica napus L.) under three K levels. The main results were as follows. (1) The K content of seed was high at former stage of seed filling, then decreased gradually as the growing of seeds. (2) The Richards equation could be used to describe the process of K accumulation in seeds (R2=0.9963**~0.9981**). (3) The time of early stage was longest among three stages in all treatments, and the ratio contributing to the whole time of K accumulation was about 55%. The rate and amount of K accumulation were highest in middle stage. The ratio of amount of K accumulation at middle stage was above 50%, and at early stage, late stage was about 40%, 13%, respectively. (4) The whole time and total amount of K accumulation increased as the increasing of k application, but effect was not significant.

  • 土肥植保
    ZHANG Xing-mei, WANG Wei-li, HE Shu-ping, ZENG Ling-ling, HONG Yin
    University, Daqing 163319, Heilongjiang )
    2007, 29(4): 452-455.
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    In order to learn the effect of potassium fertilizer on soybean in different soil types, field experiment was carried out to study the effect of potassium application rate on the growth and development, yield and quality of soybean in Black soil and Albic soil. The result showed that adequate potassium application rate could increase chlorophyll content of soybean, it could strengthen photosynthesis and settle the foundation of high yield. There was a quadratic regression equation between potassium rate and yield of two soils. In Black soil: =2516.1+6.6307X-0.0242X2 (R2 =0.8475 ); In Albic soil: =2453.1+4.2819X-0.0140X2 (R2=0.9836 ). According as the correlation of potassium application rate and yield, and combine the economic benefit of fertilizers, the maximum potassium application rate should be less than 140kg/hm2 in Black soil and 150kg/hm2 in Albic soil. The application of potassium fertilizer had a tendency that protein content decreased and fat content increased. Potassium fertilizer can improve the total content of protein and fat. In the test, the total protein content were increased 25.4% and 11.1% than CK in Black soil treatment 3 and in Albic soil treatment 4; the total fat content were increased 15.3% and 14.7% than CK in Black soil treatment 3 and in Albic soil treatment 4.
  • 土肥植保
    SUN Hu1,LI Shang-xia2, WANG Yue-fu1,WANG Wei-hua1, WANG Cheng-xia1
    2007, 29(4): 456-459.
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    Peanut cultivar was used to study the effects of nitrogen levels on yield and sucrose metabolism of leaves in peanut HUA YU 17 under conditions of field cultivation. The results showed that: application nitrogen could adjust glucose Synthesis of leaves in peanut. Applying proper nitrogen could increase Sucrose Phosphate Synthesis and Sucrose Synthesis activity, promote glucose Synthesis of leaves consequently increased the yield of peanut. In the certain nitrogen amount range, all composing factors levels of peanut yield increased as adding nitrogen amount, so as to improve yield;but excessive nitrogen could decline all composing factors levels of peanut yield, decrease economic coefficient fearfully and yield. From this paper, we found that : the most proper nitrogen level was about 157.75 kg/hm2 while reaching the highest yield.
  • Li Ying , YANG Miing-xiu, Wen Jing-hi
    2007, 29(4): 460-465.
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    Virulence of sixty-eight fist generation single-zoospore colonies,which were segregated from seven Phytophthora sojae isolates ,were evaluateded by using wounded excised inoculation method. Among the first generation single-zoospores, there were no one having the same virulence formula as their parents, the variation reached to 100%. The virulence of the progenies was inclined to become subdued. But the virulence of first generation of single-zoospore colonies coming from the same parents exhibited higher similarity.
  • 分析测试与加工
  • 分析测试与加工
    Wang Ming-xia, Huang Feng-hong*,Liu Chang-sheng,Wang Jiang-wei,Huang Qin-jie
    2007, 29(4): 466-469.
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    The antioxidative of natural antioxidants ,including the natural vitamin E , ellagic acid, tea polyphenols, bamboo-Leaves Flavonids , effecting on α-ethyl linolenate were studied by schaal-oven method and accelerating oxidation process in this paper. The results showed that antioxidantive effects of natural antioxidants on α-ethyl linolenate were: 90% tea polyphenols >82% ellagic acid >50% tea polyphenols > bamboo-Leaves Flavonids,andα-ethyl linolenate could be stable by adding 0.05% natural antioxidants. Compared with schaal-oven method, accelerating oxidation process is faster, more convenient and easier.
  • 分析测试与加工
    Qiao Xiao-hui1,2, Yang Mei2, Liu Chang-sheng2, Nan Zhan-dong1,2,Huang Feng-hong2*

    2007, 29(4): 470-474.
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    An experimental study on mixing effect, pressure drop, central fountain height, solids circulation and flow regime map of spout-fluidizing bed with a draft tube has been performed in a 150mm inside diameter, 1040mm height polymethyl methacrylate column with 90°conical distributor. Two kinds of bed material are used. The diameter of quart sand are 0.83~0.55, 0.55~0.38 and 0.38~0.25mm with the density of 2450 kg/m3.The diameter of oilseed rape straw is 0.85~0.43 mm and its density is 738kg/m3. It was found that fluidization could be well realized in draft tube spout-fluidizing bed when quart sand of 0.38~0.25m and oilseed rape straw and stalk were mixed together with the ratio 4:1, total weight 500g. Solid circulation velocity and central fountain height would increase while operating gas velocity was increasing. Flow regime map was set up based on the conditions of this experiment. The result is favor to the development of cold model experiment.


  • 研究简报
  • 研究简报
    Xu Ai-xia , , Xiao En-shi, Tian Guang-wen Ma Changzhen, Zhang Gai-sheng

    2007, 29(4): 475-478.
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    The crosses of yellow-seeded local variety Wuqi Yellow mustard(Brassica juncea L.) with two brown-seeded local variety Chang-an mustard and Wugong mustard which originated from Shaanxi Guanzhong were made respectively to study seed coat colour inheritance of Yellow mustard from the northwern part of Shaanxi.The inheritance of seed coat colour was investigated in the F1, F2 and BC1 of these two crosses. The results showed seed coat colour in Yellow mustard was under the control of the maternal genotype, the brown seed trait is dominant over the yellow seed trait. The segregation generations analysis revealed that the yellow seed coat colour in Yellow mustard was mainly controlled by a single recessive gene because the ratios of brown seeds and yellow seeds were 3:1 and 1:1 respectively in the F2 and BC1 of these two crosses, and expression of the seed coat colour influenced by some decorate genes and environment conditions. The correlation between seed coat color and quality trait in F2 population was analysed preliminarily. Besides closely related to the oil content the compositions of fatty acids were related to seed coat color of Yellow mustard.

  • 研究简报
    YANG Rui-ji
    2007, 29(4): 479-482.
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    In this paper, a field experiment was conducted on Lanzhou and Wuwei in China to study the effects of applying rape planting different sowing density on dynamic change trends of plant height and dry weight and fresh weight and leaf area index and net assimilation rate of wheat stubble multiple rape cropping. The results showed that aboveground organism yield accumulation rules can be described by “S” developing curve that was the seedling stage slower heightening and the buds stage quickest heightening and again slower heightening after blooming stage on wheat stubble multiple silage rape. Increasing sowing density significantly increased dry weight and fresh weight along with their daily growth rate of multiple-cropping silage rape with wheat, moreover, 11.5 kg/hm2 sowing density was a the best yield project. Increasing sowing density obviously improved leaf area index and net assimilation rate of wheat stubble multiple rape cropping. Using mathematics equation simulated and compared the correlativity of rape growth stages and indexes in different sowing density, the results of simulated showed that dry weight was y = k/(1+ae-bx), and leaf area index was y = a+bx+cx2, other indexes all accorded with y = a+bx+cx2+ dx3. The model simulation degree was all extremely remarkable.

  • 研究简报
    Chen Bing-dong1,2,Yue Yun3,Huang Gao-bao1, jia xiu-ping1 , Che Zong-xian2,pei hui-di1
    2007, 29(4): 483-486.
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    Helianthus annuus L plants in the pots were stressed under different salinity density .The results indicated that:the Oil content of seed and Oil content of kernel decline siginificately under the effect of the salt contnt of soil ,and Oil content of seed have much influence than the Oil content of kernel. And. Oil content of kernel influence bigger than Oil content of seed .The increase of the salt content of soil improved the production of Palmitic acid and Linoleic acid,pressing that of oleic acid.but the influence of Stearic acid and Linolenic acid under salt content of is not singnificant .the influence of the salt content of the component of the oil content of kernel ,as follow: Palmitic acid>oleic acid> Linoleic acid> Stearic acid> Linolenic acid.
  • 研究简报
    YANG hong-fu1 ,WANG zhi-yuan 1, ZHANG shi-jun 2
    2007, 29(4): 487-499.
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    Objective of this study is to investigate effects of ZPT(2-mereaptopyridine-N-oxide zine ), a fungicide derived from plants, on Sclerotinia mycelial growth and the disease control. The results indicated that ZPT the fungicide could strongly inhibit on mycelial growth of sclerotiorum ,and its EC50 value was 1.5µg/mL. In the experiment of detached leaf inoculation with mycelial agar discs, disease lesions reduced 72% when ZPT suspension at 10 ug/mL was applied onto leaf surface and when ZPT concentration increased to 500 infection was completely inhibited. In field experiments, the control effect of 25%ZPT (wettable powder ) against sclerotinia disease in oilseed rape reached about 80% when foliar spray at the flowering stage. it was used as foliar spray at the flowering stage.
  • 综述与专论
  • 综述与专论
    GUI Qin1, WANG Jia-lu3, WU Xiao-ming2, WANG Jian-bo1
    2007, 29(4): 497-502.
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    Detection of differential expressed gene is a newly focus in study of functional genomics. Several methods for identifying differences in gene expression were developed by many researchers, including SSH, RAD, SAGE, DDRT-PCR, AFLP-cDNA, cDNA-microarray. SRAP is a newly developed molecular marker system. Generally, SRAP was used to fingerprint genomic DNA while seldom to fingerprint cDNA. The principle, advantage, and applications of SRAP-cDNA method were introduced in this article. The results indicated that SRAP-cDNA method had several advantages over other systems: simplicity, reasonable throughput rate, highly reproducibility and inexpensiveness. It could be widely used in analysis of differential gene expression in plants, and could be a powerful tool for study of crop breeding and heterosis.