Welcome to CHINESE JOURNAL OF OIL CROP SCIENCES,

Archive

  • 2014 Volume 36 Issue 6
    Published: 28 December 2014
      
  • Select all
    |
  • QTL mapping of plant height using high density SNP markers in Brassica napus
    ZHANG Feng-qi, LIU Yue-ying, CHENG Xiao-hui, TONG Chao-bo, DONG Cai-hua, TANG Min-qiang, HUANG Jun-yan*, LIU Sheng-yi
    2014, 36(6): 695. https://doi.org/10.7505/j.issn.1007-9084.2014.06.001
    Download PDF ( )   Knowledge map   Save
    Plant height is an important factor affecting the yield of oilseed rape. In order to find the markers linked with plant height and to increase the efficiency of plant height improvement by molecular marker-assisted selection, identification of quantitative trait loci (QTL) with single environment and multi-environment detection methods for plant height was conducted in a recombinant inbred lines (RILs) population derived from a cross between 888-5 (a dwarf line) and M083 (a tall line) by employing the newly developed Brassica napus 60K SNP array. The results indicated that a total of 27 putative QTLs for plant height in the 4 environments were detected on the chromosomes A2, A4, A5, A6, A9, A10, C3 and C7, each accounting for 0.70% - 26.10% of the phenotypic variation. Among them, 6 QTLs showed interaction with the environment, and their effect values in the 4 environments were different, but their directions were consistent. QTL qPHE2-4 which had the largest effect in the 27 QTLs was detected in 2 environments, explaining 17.96%-26.10% of the phenotypic variation, and 2 linkage SNP markers in this QTL region had less than 0.2 cM distance from the peak of the QTL．These QTLs provided useful information for improving the plant height in oilseed rape breeding.
  • Cloning, prokaryotic expression and bioinformatics analysis of polygalacturonase-inhibiting protein gene (Bnpgip2-1) from rape
    CHEN Xi-jun, ZHANG Lei, CHEN Yu, ZHANG Jia-hao, TONG Yun-hui, XU Jing-you*
    2014, 36(6): 701. https://doi.org/10.7505/j.issn.1007-9084.2014.06.002
    Download PDF ( )   Knowledge map   Save
    According to the sequence of GenBank and related references, the open reading frame of gene Bnpgip2-1 (GenBank accession number KJ820998) was amplified, which was 1 068 bp, 98% similarity to Bnpgip2, containing a single intron of 72 bp (position 544-615) and a coding region of 996 bp. 4 restriction enzymes cutting sites of Ava Ⅰ (185), Hind Ⅲ (536), EcoR Ⅰ (929) and ApaL Ⅰ (972) were in this gene. Prokaryotic expression product of the gene could inhibit the polygalacturonases (PGs) activities of Sclerotinia sclerotiorum, the pathogen of rape Sclerotinia stem rot. The inhibition ratio was 52.96%. Bioinformatics result showed that BnPGIP2-1 was a 331 amino acids hydrophobic protein with theoretical molecular weight 36.99 kDa and pI 8.35. The protein was mainly located on cell wall. Its signal peptidesplice site was between 22th and 23th amino acid residue. 5 and 3 cysteines were in N- and C- terminal, forming 3 disulfide bonds. The main structural elements of the deduced protein, which showed the typical leucine-rich repeat (LRR) modular organization, were α-helix, β-sheet and random coil. Tertiary structure was a right-handed helix, consisted of 10 repeated LRR modular organizations, and formed an opening activity cleft which might be responsible for the interaction of PGIP and PGs from the pathogen. Bnpgip2-1 expression was strongly induced by pathogen infection, not affected by SA (salicylic acid), and down-regulated by JA (jasmonic acid).
  • Molecular cloning and expression of GmWUS related to regeneration in soybean
    ZHOU Yang, LIU Wei, SUN Shi, HAN Tian-Fu, HOU Wen-Sheng*
    2014, 36(6): 707. https://doi.org/10.7505/j.issn.1007-9084.2014.06.003
    Download PDF ( )   Knowledge map   Save
    WUS plays an essential role in plant regeneration. To preliminarily understand the function of GmWUS, based on the soybean database, the related bioinformatics analysis was conducted. GmWUS and AtWUS proteins were different in the acidic region, while other domains were conservative. Meanwhile, GmWUSa full-length sequence had been cloned. The protein mainly localized in the nucleus, showing that it might be a homeodomain transcription factor. The expression of GmWUS in cotyledons had been detected by qPCR during seeds germination. The results showed that both GmWUSa and GmWUSb expression levels kept rising, but GmWUSa expression decreased in the late stage. GmWUS expression in cotyledon node during cluster buds induction showed an approximate periodic pattern, indicating that GmWUS involved cluster buds differentiation and regeneration. Comparing the expression of GmWUSa with GmWUSb, they might play a temporal complementary in their functions. The results in this study will help to investigate roles of GmWUS in soybean regeneration, to explain the mechanism of cluster buds formation, and to promote soybean regenerative capacity so as to improve the genetic transformation efficiency by over expressing this gene.
  • Interactive regulation between expression of GmNMHC5 and phytohormones in soybean roots
    ZHAO Zhen-fang, LIU Wei, ZHENG Li-ping, FENG Yong-jun*, WU Cun-xiang*
    2014, 36(6): 713. https://doi.org/10.7505/j.issn.1007-9084.2014.06.004
    Download PDF ( )   Knowledge map   Save
    To explore the relationship between GmNMHC5, a member of MADS-box transcription factor family, and the production of phytohormones in the roots of soybean (Glycine max) cv. Zigongdongdou, GmNMHC5 was transferred to soybean plant by Agrobacterium rhizogenes as mediate, the positive transgenic hairy root were selected with GUS staining and relative expression level of GmNMHC5. The content of phytohormones in the transgenic and non-transgenic hairy root (CK) were detected using ELISA method at V3 stage. The results indicated that in comparison with control, gibberellin A3 (GA3) increased 18.5%, and ABA content decreased 19.6% respectively. These indicated that the over expression of GmNMHC5 promoted the production of GA3 and suppressed the production of ABA. After treatment with exogenous GA3 (50μmol/L) and exogenous ABA (50μmol/L), real-time fluorescent quantitative PCR analysis showed relative expression of GmNMHC5 in root were 4.52 and 0.31 times of the control at V3 stage. It was suggested that the promotional effects of exogenous GA3 and suppressive effects of ABA on the expression of GmNMHC5 were both highly significant in the root of soybean respectively. These data revealed that there was an interactive regulation between the expression of GmNMHC5 and the production of phytohormones in soybean.
  • Isolation and functional characterization of soybean chalcone reductase gene Gmchr4
    ZHANG Zhuo, MA Jian, FU Yong-Ping, YAO Dan, QU Jing, WANG Pi-wu*
    2014, 36(6): 720. https://doi.org/10.7505/j.issn.1007-9084.2014.06.005
    Download PDF ( )   Knowledge map   Save
    Chalcone reductase (CHR) is a key enzyme in the biological synthesis of daidzein, which is the main composition of isoflavones. In this study, chalcone reductase gene Gmchr4 (Genbank code: KF938604) was isolated from the soybean Jinong17. This gene consisted of a 1410bp fragment including an open reading frame of 969bp. Phylogenetic tree of CHR protein was performed by using MEGA4.0 program. The Gmchr4 gene was transformed into tobacco, and the presence of isoliquiritigenin in tobacco was confirmed by high-performance liquid chromatography (HPLC). Expression of Gmchr4 gene under the control of 35S CaMV promoter was also examined by real-time fluorescence quantitative PCR (Q-PCR). Cloning and characterisation of Gmchr4 gene were beneficial to elucidate the biological synthesis and regulation of soybean isoflavones, and to manipulate the phenylpropanoid pathway generated the isoflavonoid phytoalexin.
  • Identification of hybrid rapeseed using SSR markers
    ZHANG Bing-qing, LU Xu-zhong*, WU Xin-jie, LI Li, CHEN Feng-xiang, MA Lin, ZHANG Xiao-juan, NI Jin-long, WANG Xiu-feng, QIN Rui-ying, YANG Jian-bo*
    2014, 36(6): 728. https://doi.org/10.7505/j.issn.1007-9084.2014.06.006
    Download PDF ( )   Knowledge map   Save
    For new hybrid rapeseed (Brassica napus L.) identification, previous 33 pairs of SSR markers were used to identified 12 breeding lines and commercial cultivars. Results showed that 3 patterns of SSR markers types with 4-22 distinct markers were obtained from the samples. They were identical type, suspected identical type and distinct type. Among the 12 materials, 3 half-sib hybrids with the same paternal parent exhibited 4-16 distinct markers, and 4 half-sib hybrids with same maternal parent exhibited 7-18 distinct markers. Furthermore, among the commercial hybrids from market, 12-20 distinct markers were found. 0-2 distinct markers occurred between 2 samples with the same hybrid. Therefore, for identifying genuineness of rapeseed hybrids by using 33 SSR markers, the sample could be recognized as the same hybrid or very similar hybrid if the distinct markers were less than 2. That would be the threshold for identifying as different hybrids. The feasibility of this method was further proved by blind identification.
  • SSR markers related to pod characteristics in new peanut cultivar Zhonghua 16 with high yield and oil content and its direct and indirect parents
    CHENG Liang-qiang, HUANG Li, REN Xiao-ping, CHEN Yu-ning, ZHOU Xiao-jing, JIANG Hui-fang, LEI yong, HUANG Jia-quan, YAN Li-ying, LIAO Bo-shou*
    2014, 36(6): 735. https://doi.org/10.7505/j.issn.1007-9084.2014.06.007
    Download PDF ( )   Knowledge map   Save
    Pod traits including pod length and width, seed length and width, 100-pod weight, and 100-seed weight of a high yield and high oil content novel peanut cultivar Zhonghua 16 and 8 related parental lines in its breeding pedigree were investigated. The results indicated that the pod length, 100-pod weight and 100-seed weight of Zhonghua 16 were lower than the corresponding data of the immediate parental genotypes, Zhonghua 5 and 8130, respectively but higher than the corresponding means of all 8 parental lines respectively. The pod width and seed width of Zhonghua 16 were higher than that of the 8 parental lines in the pedigree respectively. The seed length of Zhonghua 16 were higher than that of the Zhonghua 5 and 8130 and the average of the 8 parents respectively. Based on the results of PCR analysis using 136 polymorphic SSR primers, 64.1% and 87.3% polymorphic bands of 8130 and Zhonghua 5 were identified in Zhonghua 16. Besides, there were some bands in Zhonghua 16 which were absent in both direct parents 8130 and Zhonghua 5. Certain candidate SSR markers related to the pod traits were identified including 5 markers related to 100-pod weight, 4 related to 100-seed weight, 3 related to pod length and 3 related to seed length. Several SSR bands related to large pod including IPAHM606-1, IPAHM531-1, GA8-2, 1B9-4 and HAS0428-2 were observed to have been inherited from the parents to Zhonghua 16.
  • Morphological variation of root traits in a rapeseed core collection and its effects on yield
    WU Jin-feng, GAO Gui-zhen, LI Feng, CHEN Bi-yun, XU Kun, CAI Meng-xian,ZHOU Meng-yan, WU Xiao-ming*
    2014, 36(6): 741. https://doi.org/10.7505/j.issn.1007-9084.2014.06.008
    Download PDF ( )   Knowledge map   Save
    To detect morphological variation of root traits of rapeseed (Brassica napus L.) and to clarify the relationship between root traits and seed yield, a collection of 307 accessions of rapeseed core germplasm from all over the world were used as materials. At seedling stage, rapeseed roots were classified into 8 types (type A to H) based on root morphological characterization. At seedling, flowering and mature stages, fresh root and shoot weight, taproot length, root crown diameter and primary lateral roots number were recorded. Significant correlations were found among these root traits. Correlation coefficient between seedling root fresh weight and root crown diameter at flowering stage was the highest at 0.61. Stepwise regression analysis suggested root traits and fresh shoot weight could explain 8.7%, 13.3% and 3.9% of seed yield variation at these 3 stages respectively.
  • Screening and evaluation of waterlogging tolerance in Brassica germplasm resources
    XU Jing1,2, ZENG Liu1, XU Ming-yue1, CHENG Yong1, ZHANG Xue-kun1, ZOU Xi-ling1*
    2014, 36(6): 748. https://doi.org/10.7505/j.issn.1007-9084.2014.06.009
    Download PDF ( )   Knowledge map   Save
    To fully understand Brassica germplasm of waterlogging tolerance, 270 rapeseed materials from rapeseed industrial systems were evaluated for waterlogging tolerance based on relative survival rate (RSR) at germination stage after 12h of waterlogging. Results demonstrated rich genetic diversity of waterlogging tolerance, and the tolerance varied evidently among different types of varieties. The waterlogging tolerance of Brassica rapa was much stronger than that of B. napus. Varieties of rapeseed in downstream region of Yangtze River were more tolerant than those in upstream and midstream regions, and the tolerance was not significant between varieties of upstream and midstream regions. Waterlogging tolerance of spring rapeseeds was stronger than those of winter and semi-winter rapeseeds. Compared to hybrid rapeseeds, conventional rapeseeds showed better tolerance to waterlogging. Based on this study, we could speed up and simplify the screening of rapeseed materials for waterlogging tolerance breeding. Thus the waterlogging breeding lines could be selected according to specific environments and growth periods.
  • Sequence diversity of resistance gene to soybean cyst nematode of SHMT among wild soybeans
    YUAN Cui-ping，ZHAO Hong-kun，WANG Yu-min，LIU Xiao-dong，QI Guang-xun，DONG Ying-shan*
    2014, 36(6): 755. https://doi.org/10.7505/j.issn.1007-9084.2014.06.010
    Download PDF ( )   Knowledge map   Save
    SHMT was cloned from soybean variety Forrest and reported as a SCN resistance gene which is at Rhg4 locus. SHMT of 92 wild soybean accessions were sequenced and their sequence diversity was analysis. A total of 13 SNPs (7 transitions and 6 transversions) and 4 Indels (all in the noncoding region) were identified, 11 of them were parsimony informative sites. Sequence diversity analysis among 92 wild soybean genotypes showed π=0.00061 and θ=0.00106 for SHMT. The 17 DNA variations formed 16 haplotypes, of which 9 haplotypes existed in two or more wild soybeans, haplotype diversity was 0.855. 3 neutrality tests (Tajima’sD, Fu and Li’s F, and Z test) were not significant for the gene in these 92 wild soybean genotypes, suggesting there was no selection pressure on it. ?2 text for distribution of alleles in resistant and susceptible accessions indicated that SHMT had no correlation with wild soybean resistance to soybean cyst nematode. 92 wild soybeans were grouped into 6 clusters using UPGMA method, some relationship was found between their origin and their genetic relationship.
  • Effects of NaHSO3 on photosynthetic characteristics and nitrogen metabolism of rapeseed seedlings
    LI Jun, LIU Li-xin, ZHANG Chun-le, GUAN Chun-yun*, DAI Lu-lu, ZHANG Li-yan, TAN Tai-long, MA Ni, YUN Jin-zhan
    2014, 36(6): 761. https://doi.org/10.7505/j.issn.1007-9084.2014.06.011
    Download PDF ( )   Knowledge map   Save
    To understand the mechanism of NaHSO3 as a photosynthetic accelerator on rapeseed, the effects of NaHSO3 on growth, nitrogen metabolism and photosynthetic characteristics of Brassica napus cultivar Zhuangshuang 11 were investigated in liquid solution added with NaHSO3 at the concentration of 0-0.08 mmol/L. Results showed that with 0.02 mmol/L NaHSO3, plant height increased by 21.0%, 28.4% and 40.4% respectively after 10, 15 and 20 days. Their fresh weight and root length were also increased but not significantly different to the control. With 0.08 mmol/L NaHSO3, root length was significantly decreased by 15.1-16.5% after 10-15 days. Contents of chlorophyll b and total chlorophyll in rapeseed seedlings significantly increased for all concentrations of NaHSO3 treatment. Increase of chlorophyll a was observed only at 0.02 mmol/L NaHSO3 treatment, and the highest contents of chlorophyll b and total chlorophyll were achieved at this treatment. After NaHSO3 treatment, photosynthetic rate (Pn), light saturation point (LCP) and apparent quantum efficiency (AQY) increased significantly, while respiration rate (Rp), intercellular concentration of CO2 (Ci) and transpiration rate (Tr) declined significantly. With a higher maximum light energy conversion rate (Fv/Fm) and actual light energy conversion rate (ΦPSⅡ) and a lower non-photochemical quenching coefficient (NPQ), the low concentration of NaHSO3 resulted in an increasing of the share of light energy which was used to describing photochemical transfer. The activity of nitrate reductase (NR) and glutamine synthetase (SR) increased after NaHSO3 treatment. In conclusion, NaHSO3 improved the photosynthesis through the increase of content of chlorophyll b, which led to a higher efficiency of electronic absorption and transportation.
  • Growth and physiological characteristics of seedlings of a drought-resistant mutant soybean under drought stress
    MO Jin-gang, MA Jian, SHEN Yong, ZHANG Li-hui,WANG Pi-wu,*
    2014, 36(6): 770. https://doi.org/10.7505/j.issn.1007-9084.2014.06.012
    Download PDF ( )   Knowledge map   Save
    To evaluate the growth and physiological characteristics of drought-resistant soybean mutant M18, 0%, 5%, 15%, 25% of PEG-6000 were applied at soybean seedling stage as drought stress conditions. JN18, a wild-type line was selected as control. The results showed that taproot length, number of lateral roots, total length of lateral roots, root volume, root dry weight of both M18 and JN18 increased initially and then decreased with the increasing of PEG concentration; the plant height, crown fresh weight, crown dry weight and RWC gradually decreased; the ratio of root/shoot, Pro content, MDA content, SOD activity and POD activity gradually increased. There was highly significant difference between dry root weight, SOD activity of M18 and J18. M18 had higher water retention, osmotic adjustment ability and enzymatic antioxidant capacity than those of J18 under the same drought stress. These morphological indexes and physiological characteristics including root volume, plant height, crown dry weight, and Pro content,could be used to evaluate drought resistance of soybean．
  • Effect of meteorological factors on yield of relay strip intercropping soybean in hilly area of the central Sichuan Basin
    YUAN Jin, LUO Qin-Ming*, LIU Wei-Guo, YANG Chen-Yu, YANG Wen-Yu, LUO ling, ZOU Jun-Lin1,WANG Xiao-Chun, YONG Tai-Wen, YANG Feng
    2014, 36(6): 777. https://doi.org/10.7505/j.issn.1007-9084.2014.06.013
    Download PDF ( )   Knowledge map   Save
    To explore the effect of meteorological factors on yield of relay strip intercropping soybean in hilly area of the central Sichuan Basin in different experimental locations. We studied the difference of yield traits of relay intercropping soybean in different experimental locations, then analyzed the effect of meteorological factors on yield components and yield. The results suggested that: (1) in hilly area of the central Sichuan Basin, higher accumulated temperature was conducive to the yield formation of relay strip intercropping soybean, while excessive rainfall, humidity and insufficient sunshine restricted the yield formation; (2) at flowering and pod bearing stages, higher relative humidity and accumulated temperature were conducive to flowering and pod bearing, while excessive sunshine hours, average daily temperature had a negative effect on flowering and pod bearing; (3) Meteorological factors showed little effect on effective number of plants and seed per pod, it affected yield by influencing pods per plant and 100-seed weight: (4) Soybean yield showed the tendency of gradually decreasing along with the delayed sowing date, early sowing was good for yield formation.
  • Effect of dark and mechanical stimulation on phytohormones content in peanut gynophore
    LI Zhen, LIU Wei*, WANG Qing-guo, ZHANG Gui-lin
    2014, 36(6): 784. https://doi.org/10.7505/j.issn.1007-9084.2014.06.014
    Download PDF ( )   Knowledge map   Save
    Peanut has a fascinating growth habit of geocarpy, such as flowers develop in the air while fruits in the soil. And the development of gynophore is critical in the process of peanut reproductive growth. Once the gynophore penetrated into the soil, the processes of ovary expansion and pod development were promoted. In this study, the contents of different phytohormones, such as IAA, GA, ABA and BR, were measured and the trends their variation were examined. The results showed that there was almost no significant difference of IAA content in gynophore under different growth conditions, while the content of GA, ABA and BR decreased under aerially grown combined with mechanical stimulation and/or in dark condition. These results indicated that the gynophore could respond to different environmental conditions by regulating the metabolism of phytohormones and their related signal transduction pathways, which further controlled the process of geocarpy. This research will assist our understanding of the internal mechanisms of gynophore development of peanut, and the breeding in improving the varieties and qualities of crops.
  • Identification of Fusarium acuminatum causing soybean root rot in Heilongjiang Province
    ZHI Ye, SUN Fei-fei, SUN Su-li, DUAN Can-xing, ZHU Zhen-dong*
    2014, 36(6): 788. https://doi.org/10.7505/j.issn.1007-9084.2014.06.015
    Download PDF ( )   Knowledge map   Save
    5 Fusarium isolates like-as Fusarium acuminatum were isolated from diseased soybean plants with root rot symptoms collected from Heilongjiang Province in 2012. Based on morphology, EF-1α gene sequencing and detection of F. acuminatum specific markers, these Fusarium isolates were identified as F. acuminatum. Pathogencity of these 5 isolates on soybean was tested by inoculating cultivars Williams and Hefeng 25 at seedlings, all isolates were strong pathogenic and no pathogenic difference was found among the isolates. To our knowledge, this is the first report of F. acuminatum as a pathogen on soybean in Heilongjiang province.
  • Pathogen identification of leaf spot disease of oil palm seedling and determination of its biological characteristics
    ZHENG Li，YANG Xing-yu, XIE Chang-ping，LI Jing1,，FENG Chao-yan2, ZENG Xian-hai，TIAN Wan-ying，SHEN Hui-fang*, QIN Xin-dao*
    2014, 36(6): 793. https://doi.org/10.7505/j.issn.1007-9084.2014.06.016
    Download PDF ( )   Knowledge map   Save
    Pathogen of oil palm leaf spot disease was isolated and purified, and referred to as strain YZ-4. According to its morphological characteristics and the sequence of internal transcribed spacer (ITS), the strain YZ-4 was identified as Pestalotiopsis microspore. Results of biological characteristics showed that the optimum temperature and pH for YZ-4 mycelia growth were 28-30℃ and 6.0-8.0 respectively, while the highest rate of conidium germination occurred at 25℃, 28℃ or 30℃, and pH 6.0-9.0. The most efficient utilized carbon sources by mycelium were D-maltose and soluble starch. When soluble starch, D-maltose, D-galactose and glucose were used separately as carbon sources, YZ-4 conidial germination rates were 94.33%, 88.33%, 90.00%, and 89.67% respectively. These rates were not significantly different. Peptone was the most efficient nitrogen source. As peptone and potassium nitrate were separately used by YZ–4 as nitrogen sources, the corresponding conidial germination rates were 80.33% and 74.00% respectively.
  • Heterology strategy-based competive indirect enzyme-linked immunosorbent assay for aflatoxin B1
    LI Min,MA Fei, LI Pei-wu*, ZHANG Qi, WANG Heng-ling, ZHOU Hai-yan,YIN Nan-ri,WANG Heng-ling,WU Hui
    2014, 36(6): 801. https://doi.org/10.7505/j.issn.1007-9084.2014.06.017
    Download PDF ( )   Knowledge map   Save
    To improve the sensitivity of enzyme-linked immunosorbent assay for aflatoxin B1, indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed with selective antibody 3G1. The procedure was modified by working preoperatively under dry conditions and using glycolic acid as a spacer providing a nucleophilic hydroxyl group for formation of aflatoxin acetal AFB2-GA in 8-position on one side. The structure was successfully identified by MS. The ESI-MS identification demonstrated that [M+H]+ was 388.07. Active ester method (AEM) was used to couple the acetal of aflatoxin B1 to carrier protein (BSA ) to obtain artificial antigen for aflatoxin B1. Hapten was then linked to BSA through active ester，and this was confirmed by UV spectrum. A result of UV spectra of AFB2-GA-BSA was obviously different from BSA and aflatoxin B1, showing that the preparation of artificial antigen for aflatoxin B1 was successful. The coupling ratio of AFB1 to BSA was 8.45:1 in the conjugate. Consequently, using novel AFB2-GA-BSA as envelope antigen, indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) method was developed with selective antibody 3G1 for aflatoxin B1. Sensitivity of 0.3ng/mL for 3G1 was achieved by heterologous ELISA with working range of 0.03-1.08ng/mL, which was 81.25% higher than that obtained by homologous detection. 12 peanut samples were analyzed by this method, which fitted well with standard curve of y = 1.044 4x + 0.090 2 and corresponding coefficient of determination R2 = 0.975 1, comparing with the result by high-performance liquid chromatography (HPLC).
  • Effects of aeration on growth and lipid productivity of Desmodesmus sp.CHX1
    CHENG Hai-xiang, SHAO Yu, LI Jian-hui, XU Tian-you, TIAN Guang-ming
    2014, 36(6): 807. https://doi.org/10.7505/j.issn.1007-9084.2014.06.018
    Download PDF ( )   Knowledge map   Save
    To promote growth and lipid production of microalgae for biodiesel, aeration effect was studied on growth of a microalga Desmodesmus sp.CHX1 cultured in BG-11 medium. Results showed that aeration could dramatically increase the algal biomass, Chlorophyll (a+b) content and lipid productivity. After 7d cultivation with the aeration 160L/h, the maximum biomass of CHX1 cell, Chlorophyll (a+b) content and lipid productivity reached 7.26g/L, 60.5mg/L and 128.7mg/(L?d) respectively, which were significantly higher than those of control. Results suggested that aeration was favorable for the growth of microalgae and accumulation of valuable additional materials during the cultivation of Desmodesmus sp. CHX1, and was also for enhancement of cell growth and lipid production.
  • Effect of silicon on the morphological and physiological characteristics of peanut seedling under aluminum stress
    YAO Xin, LI Jing, HE Xia, LIU Hong, SHEN Xue-feng
    2014, 36(6): 814. https://doi.org/10.7505/j.issn.1007-9084.2014.06.019
    Download PDF ( )   Knowledge map   Save
    In order to investigate the effect of silicon (Si) on the growth and development of peanut seedling root under aluminum (Al) stress conditions, hydroponic peanut (Arachis hypogaea L. Yueyou 7) was used in this study. The results showed that the root activity, root surface, root volume, root fresh weight, and silicon content increased by 50.4%, 29.6%, 15.7%, 9.5% and 44.7%, but the activity of CAT, SOD and POD, the content of MDA and the content of aluminum decreased by 39.1%, 35.3%, 31.9%, 18.0% and 23.5% with silicon under aluminum stress. The absorption of aluminum elements reduced, the growth of peanut root improved, the activity of antioxidant enzymes and lipid peroxidation decreased with Si under Al stress conditions. This also explained the role of silicon in alleviating the aluminum stress.
  • Response of root protective enzymes to waterlogging at different growth stages of peanut
    TAN Hong-jiao, LIU Deng-wang*, LI Lin*, MA Jie1,2,XIAO Mi,WANG Jing
    2014, 36(6): 818. https://doi.org/10.7505/j.issn.1007-9084.2014.06.020
    Download PDF ( )   Knowledge map   Save
    In order to research the response of root protective enzyme (SOD, POD, CAT) and MDA on the different growth stages of 2 different typical yield waterlogging tolerance peanut varieties, Yuhua 15 (waterlogging tolerance) and Hua 269 (sensitive), simulation experiment was used under rhizosphere waterlogged. Results showed that, there was a big difference in the response of waterlogging on different varieties and growth stages of peanut. SOD, POD and CAT activity declined, but MDA content increased in Yuhua 15; SOD activity first increased and then decreased, but CAT activity first decreased and then increased, while POD activity and MDA content increased in Hua 269 after peanut waterlogged at the seedling stage. SOD, POD and CAT activity first increased and then decreased, while MDA content reduced in Yuhua 15; SOD, CAT activity and MDA content first decreased and then increased, yet POD activity increased in Hua 269 after peanut waterlogged at the flowering stage. Conclusion: Waterlogging had a negative impact on root internal physiology, and it had greater impact in seedling stage than that in the flowering stage. The waterlogging tolerance of Yuhua 15 was weaker than Hua 269 in seedling stage but stronger in flowering stage. MDA content in the roots of peanut varieties strengthened waterlogging tolerance, While the activities of protective enzymes (SOD, POD, CAT) appeared more complicated and less predictable.
  • Genome behavior of cultivated Brassica allotetraploids and development of alien additions
    SHAO Yu-jiao, DING Meng-si, LI Zai-yun*
    2014, 36(6): 823. https://doi.org/10.7505/j.issn.1007-9084.2014.06.021
    Download PDF ( )   Knowledge map   Save
    Cultivated Brassica species are widely used to study genetic behavior of progenitor genomes during process of interspecific hybridization and allopolyploidization. This article reviewed advances in cytology and genetics during the formation and evolution of Brassica allotetraploids. Under induction of chromosome elimination in intergeneric crosses with other crucifers, 3 allotetraploids showed the hierarchy of genome stabilities as Brassica carinata>B. juncea>B. napus, while the 3 genomes (A, B and C) presented the hierarchy of B>A>C. B. napus (AACC) tended to lose more and C-genome chromosomes, but the B-genome chromosomes in other two allotetraploids were preferentially maintained. The genetic and epigenetic changes in natural and resynthesized allotetraploids were affected by their cytoplasm types and genome combinations. The alien additions of Brassica species with individual chromosomes from other crucifer were also introduced.
Copyright © CHINESE JOURNAL OF OIL CROP SCIENCES, All Rights Reserved.