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    Variation trend of rapeseed production in China from 2008 to 2019
    LENG Bo-feng, LI Xian-rong, CHEN Xue-ting, TANG Jing, CHEN Zhuo-er, ZHANG Qing, WANG Tao, TIAN Yu, WEI Meng-sheng, YAN Hua, LIU Cheng, FENG Zhong-chao
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 171-.   DOI: 10.19802/j.issn.1007-9084.2019320
    Abstract246)      PDF (26142KB)(743)      
    To better understand the variation on rapeseed traits in recent 12 years in China, and to provide sug⁃
    gestions for high yield cultivation of rapeseed cultivars, yield data of 7916 samples from 18 provinces with fixed ob⁃
    servation points of China Agriculture Research System (rapeseed) from 2008 to 2019 was collected. Descriptive sta⁃
    tistical method was used to report the variation trend of planting pattern, planting density, plant height, branch, fruit⁃
    ing, yield per unit area and disease of rapeseed in China over the past 12 years. The results showed that, during the
    past 12 years, direct seeding percentage increased by 30.74 points, planting density increased by 56.66%, effective
    branches decreased by 16.05%, first effective branch height increased by 50.20%, plant height increased by 8.82%,
    effective pod per plant decreased by 22.14%, seeds per pod increased by 3.02%, 1000-seed weight decreased by
    0.62%, average yield per plant decreased by 22.38%, theoretical yield increased by 15.00%, actual yield increased
    by 14.03%, cold injury index decreased by 13.57 points, sclerotinia stem rot desease index decreased by 0.83
    points, other pests and diseases index increased by 2.34 points. It was suggested that in the future, high-yieldingrape cultivars should be planted with more density tolerance, more branches, more effective pods per plant and more
    suitable for environments. Meanwhile, according to the actual situation of each region, the cultivation method of
    rapeseed should be guided especially on planting density.

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    Effects of COVID-19 on China's rape industry and strategies
    ZHANG Qing, LENG Bo-feng, CHEN Xue-ting, CHEN Zhuo-er, WEI Meng-sheng, FENG Zhong-chao, LI Xian-rong
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (4): 551-.   DOI: 10.19802/j.issn.1007-9084.2020332
    Abstract229)      PDF (1101KB)(196)      

    To effectively cope with the shock of COVID-19 and its subsequent impact, and to promote stable and healthy development of rape industry, this paper systematically analyzed the impact of COVID-19 on China's rape industry. It includes perspectives of farmer's life, rape production and rapeseed processing enterprises, based on micro survey data provided by the National Rapeseed Industrial Technology System. The results showed that in terms of farmers' life, only 0.98% of the respondents were in a shortage of edible vegetable oil and 76.06% of them reflected no impact or a little impact on total household income. However, rural tourism decreased by 10.24 percentage points, and honey collecting behavior of bee farmers decreased by 4.6 points. On field production, 85.47% of the respondents reported "no change" and "small reduction" in field management workload, 90.38% reflected "no impact" and "a littlel impact" on rapeseed production employment, 79.06% considered "overall stability" in the price of agricultural materials, and 98.48% believed that it had a little impact on final output. However, the pest reporting rate increased by 4.99 points than last season. On rapeseed processing, it varied from region to region on the rapeseed processing enterprises' resumption rate, starting-up rate, specific difficulties faced by enterprises and government policies. Based on these results, future strategies should be taken to ensure improving development of rape industry. The strategies should include perfect construction of China's oil reserve system, more attention to diversification of imports, more innovation and technology promotion, optimization on marketing system and construction of information platform, and a“package”subsidy plan for the industry.

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    Research advances in the biosynthesis and regulation of lipid in oil crops
    FAN Shi-hang, LIU Nian, HUA Wei
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (3): 361-.   DOI: 10.19802/j.issn.1007-9084.2021097
    Abstract218)      PDF (2044KB)(296)      
         Vegetable oil is not only the main source of edible oil but also an important renewable biofuel for nproduction and industry for humans. Thus, it is of great scientific significance and application value to elucidate the mechanism of the biosynthesis and regulation of oil in plants, which can improve the content and composition of vegetable oil. In this review, the biosynthesis of plant oil and the progress of genetic research on oil syntheses such as maternal effect, QTL, and GWAS were summarized to uncover the mechanism of lipid accumulation of oil cropsMeanwhile, we summed up the well-known genes that participate in lipid biosynthesis and regulation. This paper was expected to shed light on current research status in this field, provide an important reference to the further understanding of the regulation of oil crops lipid biosynthesis, and to give a better theoretical basis to oil crops molecular improvement and genetic breeding.

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    Breeding and genetic background of the high-oleic peanut variety Yuhua 37
    QIN Li, LIU Hua§, ZHANG Xin-you, DU Pei, DAI Xiao-dong, SUN Zi-qi, QI Fei-yan, DONG Wen-zhao, GU Jian-zhong, HUANG Bing-yan, HAN Suo-yi, ZHANG Zhong-xin, XU Jing, GAO Wei
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (4): 608-.   DOI: 10.19802/j.issn.1007-9084.2020144
    Abstract174)      PDF (1322KB)(125)      

    Yuhua 37, a high oleic acid peanut variety with high yield, appreciable resistance to major diseases and wide adaptability, was bred by hybridization and pedigree selection. The female parent Haihua 1 was a widely grown variety and the male parent Kaixuan 016 was the high-oleic trait donor line. The near-infrared quality analyzer and molecular marker-assisted selection approach were applied in the selection of single plants in the segregating generations. In 2012 and 2013, the variety was tested in the Henan Peanut Regional Trial for High-quality Spanish-type varieties. The average yield was 4583.55 kg/hm2 with an increase of 2.68% over the control variety Yuanza 9102 across 9 test locations in two years. In 2014, it was included in the Henan Provincial Peanut Production Test for Spanish-type variety, an average pod yield of 5084.40 kg/hm2 with an increase of 10.84% over the control Yuanza 9102 was recorded across 6 test locations. This cultivar is a small-seeded Spanish-type variety with growth duration of about 116 days when planted in the summer season in Henan province. Yuhua 37 was approved to be released by Henan province in 2015. Its genetic background and the mutation types of the two major genes controlling oleic acid content were further analyzed, which may provide some enlightenment in future development of high-oleic peanut varieties.

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    Application of nanomaterials in plant genetic transformation
    GAO Hong-fei, FAN Shi-hang, LIU Jing-lin, XIONG Chong-ming, HUA Wei, LI Jun
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (1): 64-.   DOI: 10.19802/j.issn.1007-9084.2020206
    Abstract174)      PDF (396KB)(242)      
    To break the main obstacle that the cell wall preventing delivery of exogenous biomolecules into in⁃
    tact cell during plant genetic transformation, nanomaterial-mediated gene delivery technology was currently suc⁃
    cessfully applied. Traditional transgenic methods require the aid of external force or the mediation by creatures like
    agrobacterium. The nano-carriers for genetic delivery possess outstanding advantages such as excellent biocompati⁃
    bility, ability to protect external DNA/RNA against nuclease degradation, high transfection efficiency, thus provide
    a new promising way for plant genetic engineering. In this paper, we summarized the bottlenecks of plant genetic
    transformation met currently, the drawbacks of the existing methods and the advantages of nanomaterial-mediated
    delivery. And the key techniques and research progresses of nanoparticles-mediated plant transformation were dis⁃
    cussed in detail. Ultimately, the advantages and disadvantages as well as future direction and problems of this newly
    formed technology were also discussed and prospected.

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    Progress in research on mechanism of resistance to Aspergillus flavus L. infection and aflatoxin production in peanut
    CUI Meng-jie, CHAI Peng-pei, GUO Jun-jia, HUANG Bing-yan, DONG Wen-zhao, HAN Suo-yi, ZHANG Xin-you
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (4): 562-.   DOI: 10.19802/j.issn.1007-9084.2020236
    Abstract160)      PDF (1323KB)(170)      
    Aflatoxin contamination caused by infection of Aspergillus flavus L. is an important factor which poses a serious threat to production and food safety in peanut. Contamination resistance of Aspergillus flavus can be divided into two categories: infection of Aspergillus flavus and aflatoxin contamination. Resistance mechanisms are usually classified into morphological, physiological, and molecular resistance mechanism. The main factors that determine the resistance of peanut seed coat to infection are the thickness of wax layer, the arrangement density of palisade cells and the existence of seed coat cracks and so on. The contents of tannic acid, trypsin inhibitor and resveratrol were related to the resistance of peanut seeds to Aspergillus flavus infection and aflatoxin production. The pathogenesis-related genes, transcription factor genes, lipoxygenase genes, and other resistance genes are involved in the process of peanut resistance to Aspergillus flavus. In the future, it is possible to explore the mechanism of resistance to Aspergillus flavus infection and aflatoxin production of peanut seeds based on genome-wide association studies and various omics techniques.
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    Genome-wide identification and expression analysis of ZIP transporter gene family in Brassica napus
    HE Dan, YANG Tai-hua, LI Ting, WU Jin-feng, PENG Jia-shi, LIU Li-li, ZHANG Da-wei, YAN Ming-li, LI Zai-yun
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (3): 392-.   DOI: 10.19802/j.issn.1007-9084.2021071
    Abstract157)      PDF (11750KB)(312)      
        ZIP transporter gene family, which is mainly involved in absorption and transport of metal cations,and plays an important role in plant, development and response to heavy metal stress. In this study, 51 members of the ZIP gene family in Brassica napus were identified by bioinformatics method,. Tandem duplication were found in A and C subgenomes as compared with three basic species of Brassica. Using Brassica napus CV ZS11 as a reference genome, the ZIP gene family was mapped onto 17 chromosomes, and 10 motif were predicted, all of which contained ZIP domain. Moreover, the cis-regulatory elements locating at 3 kb upstream of promoter region were predicted. Based on phylogenetic tree analysis, the ZIP gene family of Brassica napus was divided into three major groups,and was conserved among the three basic species of Brassica. In addition, ZIP gene family showed tissue-specificand BnaIRT3 genes were ac⁃tively expressed in different tissues and different developmental stages. We also found that BnaIRT1.A01a was expressed in both leaves and roots of rape treated with different concentrations of cadmium, which might play an important role in response of rape to cadmium stress. These results might provide references for the subsequent biological functions of the ZIP gene family.

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    Genome-wide identification and expression analysis of AOC gene family in Brassica napus L.
    KANG Yu, LIU Wei, XIE Pan, GUAN Mei, QIAN Lun-wen, GUAN Chun-yun, HUA Wei, HE Xin
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (3): 405-.   DOI: 10.19802/j.issn.1007-9084.2021070
    Abstract154)      PDF (6183KB)(184)      
         Allene oxide cyclase(AOC)is an important synthase in jasmonic acid(JA)signal pathway, whichplays an important role in many growth and development processes, such as photosynthesis, material metabolism and stress response. In this study, based on 4 AOC genes of Arabidopsis thaliana, 12, 4 and 6 AOC genes were identified in Brassica napus, Brassica rapa and Brassica oleracea. Then the molecular characteristics, protein conserved domains, phylogenetic tree, gene structure and chromosome distribution, tissue expression patterns under different stresses and hormone treatments were systematically compared and analyzed. The results showed that AOC gene wa highly conserved in the above Brassicaceae species, and AOC1/2/3 gene existed in the form of tandem gene cluster;12 BnaAOC genes had different tissue expression patterns and responded to various stresses and hormone treatments; BnaAOC3(BnaA09g19550D and BnaC09g52550D)genes were induced by methyl jasmonate(MeJA), salicylic acid(SA), salt stress and PEG-6000 treatment, and strongly induced by Sclerotinia sclerotiorum. This study
    might lay a foundation and provide guidance for further understanding the biological functions of AOC family genesin response to abiotic stress and S. sclerotiorum in Brassica napus.

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    Preliminary study on function of CO homologs in Brassica napus
    WU Xian-ping, WANG Zhi-xin, ZHUO Chen-jian, WEN Jing, YI Bin, MA Chao-zhi, SHEN Jin-xiong, FU Ting-dong, TU Jin-xing
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 186-.   DOI: 10.19802/j.issn.1007-9084.2019266
    Abstract150)      PDF (6811KB)(238)      
    CONSTANS (CO) gene is an important transcriptional regulator in photoperiodic flowering pathway.
    To explore the function of CO homologous gene of Brassica napus, CDS sequence of Arabidopsis CO was used as ref⁃
    erence, 2 homologs (BnaC09g41990D, BnaA10g18430D) were isolated from cDNA of Brassica napus variety Westar
    and named BnaC09CO and BnaA10CO respectively. The overexpression vectors were constructed to transform into
    Arabidopsis thaliana to observe phenotype and quantitative analysis. Results showed that flowering time of overex⁃
    pression lines was 3-5 days earlier than that of wild type, and the expression of BnaC09CO, BnaA10CO, FT and
    SOC1 were significantly higher than those of wild type. These indicated that BnaC09CO and BnaA10CO played sim⁃
    ilar roles to CO, which could upregulate the expression of FT and SOC1 in Arabidopsis thaliana to promote flowering
    under long-day condition.
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    Establishment of an in vitro embryonic growth system and its application in rapeseed oil synthesis and accumulation study
    DENG Lin-bin, FAN Shi-hang, SUN Xing-chao, HUA Wei, LIU Jing
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 203-.   DOI: 10.19802/j.issn.1007-9084.2019192
    Abstract150)      PDF (25955KB)(243)      
    To facilitate the study on embryo developrnent and fatty acid accumulation, tissue culture of rape
    seeds were carried out by using Brassica napus spring type 862. Study of influence factors on 862 oil synthesis and
    accumulation showed that B5 basal medium with 8% sucrose and 0.6% agar was optimal. Extracts from pericarp
    and leaf of different varieties in the medium could improve oil accumulation efficiency in embryo. While pericarp
    extract had higher accumulation efficiency than that from leaf. Extracts of pericarp and leaf from the high oil variet⁃
    ies possessed much higher oil accumulation efficiency.

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    Comprehensive analysis on yield and quality parameters of direct-seeding rapeseed in different environments
    LI Xin-hao, LI Jun, LIU Li-xin, WANG Fang, HAO Rui, WANG Tian-yao, ZHANG Chun-lei, MA Ni
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 251-.   DOI: 10.19802/j.issn.1007-9084.2019270
    Abstract149)      PDF (2131KB)(230)      
     For stable and high yield of rapeseed in the Yangzi River area, yiled and quality of direct-seeding
    rapeseed were evaluated on the effects of density, nitrogen application and varieties in 3 different environments.
    Field experiments were conducted with 2 varieties (Brassica napus cv Zhongshuang 11 and Zhongyouza 12) under 2
    nitrogen applications (90 and 180 kg/hm2) and 3 planting densities (1.3×105, 2.6×105, 5.2×105 plants per hectare) in
    3 sites (Yichun, Jingzhou and Lu′an). Results showed that rapeseed yield had significant increase with density. The
    yield of high density (5.2×105 plants per hectare) was 25.6% higher than that of low density, and 10.8% higher than
    that of medium density. Meanwhile, high density significantly increased branch numbers per unit area, and showed
    significant positive correlation with silique number from both main and branch recemes. Compared with N2(180 kg/
    hm2), yield of N1 (90 kg/hm2) was reduced by 25.9%,36.9% and 28.9% in 3 sites, respectively. The high density
    had no significant effect on seed quality, while decrease of nitrogen fertilizer increased oil content and glucosinolate

    at Jingzhou, but reduced protein at Jingzhou and Lu′an. Comprehensive score showed extremely differences among

    the environments and cultivation conditions. Order of the investigated traits were followed by their comprehensive
    scores from high to low: aboveground dry biomass, effective siliques per unit area, branches, 1000-seeds weight, ef⁃
    fective length of main raceme, seeds per silique, and oil content.

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    Evaluation and supervision of gene editing in context of implementation of the Biosafety Law of the People's Republic of China
    WANG Pan-di, XIONG Xiao-juan, FU Ping, WU Gang, LIU Fang
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (1): 15-.   DOI: 10.19802/j.issn.1007-9084.2021020
    Abstract148)      PDF (481KB)(96)      
    On October 17, 2020, the Biosafety Law of the People's Republic of China (hereinafter referred to
    as the‘biosafety law’) passed the deliberation of the Standing Committee of the National People's Congress, and
    comes into force on April 15, 2021. Application safety of biotechnology is one of the main contents involved in the
    biosafety law. Gene editing, as a research hotspot in field of biotechnology in recent years, has attracted much atten⁃
    tion in its safety evaluation and supervision. This paper summarized the present situation of the application of gene
    editing technology, compared the different national supervision of gene editing technology, discussed the signifi⁃
    cance of biosafety law to gene editing safety assessment and supervision in China, and finally proposed suggestions
    on how to better promote and implement the biosafety law in field of gene editing biosafety.
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    An efficient and accurate method for determination of vitamin C in rapeseed
    WANG Chao, KUANG Lie-qiong, PAN Yu-ying, DUN Xiao-ling, WANG Xin-fa
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 346-.   DOI: 10.19802/j.issn.1007-9084.2019327
    Abstract147)      PDF (2103KB)(246)      
    Based on the national standard, an efficient and accurate method was used to determinate vitamin C
    in fresh samples of oilseed rape. Results showed well linear relationship between peak area and concentration of
    standard vitamin C liquid, with the correlation coefficient of 0.9999. The recovery rate of vitamin C in rapeseed was
    97.97% to 101.40%. The relative standard deviation was 1.84% only, indicating that this method had good reproduc⁃
    ibility for rapeseed vitamin C determination. Results also showed that the lower temperature during storage, the
    slower degradation rate of vitamin C. Liquid nitrogen storage was more beneficial to preservation and accurate iden⁃
    tification. The use of liquid nitrogen for sample grind was more beneficial to vitamin C extraction from the samples.
    Compared with no grinding, vitamin C content was increased by about 16%. With the extension of storage time, vita⁃
    min C content in samples stored in liquid nitrogen did not change significantly, and the relative standard deviation
    of vitamin C was less than 2%. Thus the technique could be used to study the variation of vitamin C in large popula⁃
    tion with high efficiency, accuracy and repeatable features. It was expected to be bencticial to the regetable nutrition
    and breeding research of rapeseed.

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    Evaluation on potential of reducing nitrogen and increasing efficiency for Brassica napus germplasm
    ZHANG Hao, LI Shuang, YE Xiang-sheng, ZHANG Li-mei, XU Fang-sen, SHI Lei, DING Guang-da
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 195-.   DOI: 10.19802/j.issn.1007-9084.2019282
    Abstract144)      PDF (4043KB)(173)      
    To discover the potential of reducing fertilizer and increasing efficiency from different rapeseed vari⁃
    eties, response characteristics of different genotypes of Brassica napus (L.) to nitrogen (N) fertilizer and their poten⁃
    tial of N-saving and high efficiency were analyzed by 2 years’field trials in 3 locations. Results showed that materi⁃
    als could be devided into 4 types based on average yield. The 4 types were named (1) efficiency (efficiency under
    low nitrogen condition)-inefficiency (efficiency under high nitrogen condition), (2) efficiency-efficiency, (3) ineffi⁃
    ciency-inefficiency, (4) inefficiency-efficiency. Genotypes with efficiency-efficiency and inefficiency-efficiency
    had 16.96-36.50% of N-saving potential and 21.57-49.59% of yield-increasing potential under high N condition,
    while genotypes of efficiency-inefficiency had 12.23% of N-saving potential and 10.88% of yield-increasing poten⁃
    tial under low N. In addition, Zhongshuang 9 and SV-pyriter were identified with efficiency-inefficiency. D4-15,
    Zhongshuang 11, DH16 and Huashuang 3 were recognized as genotypes with efficiency-efficiency. D2-1, Huash⁃
    uang 5, Jia99H99-15HR, Xiangnongyou 3 and DH17 were identified as inefficiency-inefficiency genotypes. 84001
    had high efficiency under high N level. These results suggested that oilseed rape had high potential of reducing N re⁃
    quirement and increasing yield, and the potential varies among varieties. Hence, identification of genotypes with ef⁃
    ficiency-efficiency and/or inefficiency-efficiency might be the target of rapeseed breeding in future.
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    Cloning and activity of BnLEC1.A07 gene promoter in rapeseed (Brassica napus L.) lines with different oil content
    WANG Yan-jia, FAN Shi-hang§, LIU Jing, ZHENG Ming, HUA Wei
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (3): 383-.   DOI: 10.19802/j.issn.1007-9084.2021092
    Abstract131)      PDF (5889KB)(160)      
         In order to further study the gene BnLEC1 on oil biosynthesis, high-oil variety 61616 and low-oil variety 51070 were used as materials to carry out transcriptome and quantitative PCR analysis. The results showed that the expression level of BnLEC1. A07 in 61616 was significantly higher than that in 51070. Cloning and sequence comparison of the promoter PLEC161616 (1829 bp) and PLEC151070 (1824 bp) were performed respectively.The results showed that a large number of SNPs and InDels between the two promoters, and single-base SNPs were abundant. Cis-acting elements analysis showed that PLEC161616 had one CAAT-box and two jasmonic acid response elements more than those of PLEC151070, and one cell cycle control element and two abiotic stress response elements were missing. The promoter fusion vectors with GUS and LUC were constructed respective. GUS staining, LUC fluorescence imaging in vivo after transient transformation of tobacco leaves and luciferase activity assay showed that the activity of PLEC161616 was higher than that of PLEC151070. The comprehensive results showed that the expression level of BnLEC1.A07 gene in different lines was positively correlated with oil content. PLEC161616 had stronger priming activity than PLEC151070. This study provides a theoretical basis for further exploring the regulation mechanism of the lipid synthesis gene BnLEC1.A07 in different rapeseed lines.

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    Genetic dissection of plant architecture-related traits by GWAS with PCA in Brassica napus
    ZHANG Xu, Safdar Luqman Bin, TANG Min-qiang, LIU Yue-ying, ZHANG Yuan-yuan, LIU Sheng-yi
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (3): 462-.   DOI: 10.19802/j.issn.1007-9084.2020032
    Abstract131)      PDF (3646KB)(175)      
         For global demand of high yield breeding, complex genetic regulation on plant architecture was focused by genome-wide association study (GWAS) and principal component analysis (PCA), based on 4 typical traits related to plant architecture (PA) of 373 B. napus (L.) accessions. The 4 traits included plant height, branch height,branch number and main inflorescence length in 3 environments (Wuhan 2014-2015, Wuhan 2015-2016, and Yangzhou 2015-2016). Results showed that PCA could reasonably explain the phenotypes related to PA. PCAGWA Sand normal GWAS could verify and complement each other and provide the most information on traits that determine rapeseed architecture. Further, 19 PA related candidate genes were obtained from chromosomes A01, A10 and C06. Two genes on chromosome C06 were related to the new loci identified by PC1-GWAS. This method and results provide new ideas and strategies for the analysis of the formation mechanism of complex traits such as PA.
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    CHINESE JOURNAL OF OIL CROP SCIENCES    0, (): 1-.   DOI: 10.19802/j.issn.1007-9084.2020335
    Abstract128)      PDF (538KB)(87)      
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    Effect of different preceding cover crops on yield and nutrient accumulation of winter oilseed rape
    YUAN Jin-chuan, WANG Wei-yan, ZHAO De-qiang, HOU Yu-ting, WU Wei, LIU Yang, WEN Xiao-xia
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 260-.   DOI: 10.19802/j.issn.1007-9084.2019311
    Abstract123)      PDF (1949KB)(179)      
    To explore the effect of preceding cover crops on winter oilseed rape (Brassica napus L.) in Guan⁃
    zhong Plain in China, a 2-year completely randomized experiment was carried out to study the impact of preceding
    cover crops. Soil nutrient content, aboveground dry matter accumulation and allocation, nitrogen and phosphorus ac⁃
    cumulation, seed yield and its components of winter oilseed rape were investigated. The test consisted of 4 treat⁃
    ments: SW (soybean as preceding cover crop), HW (hairy vetch as preceding cover crop), MW (maize as preceding
    cover crop), FW (fallow without preceding cover crop, control). Results showed that the SW and HW treatments sig⁃
    nificantly improved soil alkali hydrolyzed nitrogen, nitrate nitrogen, ammonium nitrogen and available phosphorous
    content compared to FW. Among the leguminous treatments, SW treatment has the most obvious effect of increasing
    soil nutrient content. The soil nutrients of MW were lower than those of FW treatment. In contrast to FW, SW and
    HW significantly improved annual dry matter accumulation by 26.05%, 13.12%, nitrogen accumulation by 30.86%,
    14.86% and phosphorus accumulation by 37.03%, 20.35% respectively, while MW was 2.74%, 12.76% and 3.48%
    lower respectively. Compared to FW, SW and HW treatments significantly increased annual seed yield by 29.48%
    and 17.77% respectively. MW treatment had no significant effect on seed yield. Correlation analysis showed that the
    significant effect of improving seed yield of SW and HW treatments was due to the increase of soil nutrient, aboveg⁃
    round dry matter accumulation, nitrogen accumulation, phosphorus accumulation as well as pods per plant. The
    above results suggested that soybean and hairy vetch might be the reasonable choice of preceding crop for winter oil⁃
    seed rape. Meanwhile, soybean could be recommended as the best feasible preceding crop to optimize seed yield, ni⁃
    trogen and phosphorus accumulation for winter oilseed rape in Guanzhong Plain in China.
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    Gene isolation and characterization of 5 fatty acyl-ACP thioesterase in Brassica napus
    XIONG Teng, CHEN Zhang-bin, ZHANG Zhen-qian, CHEN Hao, YUAN Yong, XIONG Xing-hua, WU Xian-meng, GUAN Chun-yun, XIAO Gang
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (2): 212-.   DOI: 10.19802/j.issn.1007-9084.2019275
    Abstract121)      PDF (1653KB)(174)      
    To better understand FAT gene function which encodes fatty acyl-ACP thioesterase, 5 FAT genes
    were cloned from Brassica napus cultivars with high oil content (HO) and low oil content (LO). They were named as
    BnaA.FATA.a, BnaC.FATA.b, BnaA.FATA.c, BnaC.FATB.a and BnaA.FATB.b. No sequence difference was found in
    these 5 genes between HO and LO. RT-qPCR analyses showed that expression levels of them in HO seeds were sig⁃
    nificantly higher than those in LO seeds. Yeast heterologous expression of them showed that oil content of all yeast
    transformants were significantly increased, and their fatty acid composition were modified. After transformed into
    Arabidopsis, seeds oil of all T1 transgenic lines were significantly increased. The saturated fatty acids contents in 3
    lines (BnaA.FATA.a, BnaC.FATA.b and BnaA.FATA.c transgenic lines) were significantly decreased, with unsaturat⁃
    ed fatty acids contents significantly increased. Conversely, in BnaC.FATB.a and BnaA.FATB.b transgenic lines, sat⁃
    urated fatty acids contents were significantly increased, and unsaturated fatty acids content was significantly de⁃
    creased. The above results indicated that expression of these 5 FAT genes could increase oil content, and modify fat⁃
    ty acid composition in transgenic Arabidopsis seeds.
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    #br# Visual analysis of yield and components of high oleic acid peanut cultivar Kainong 1760
    DENG Li, GUO Min-jie, YIN Jun-hua, GU Jian-zhong, MIAO Jian-li, LI Yang, REN Li
    CHINESE JOURNAL OF OIL CROP SCIENCES    2021, 43 (3): 502-.   DOI: 10.19802/j.issn.1007-9084.2021050
    Abstract120)      PDF (3458KB)(117)      
          To provide effective guidance for high yield and good quality of peanut breeding, yield and its rela⁃tionship with agronomic traits of small-pod high oleic acid peanut cultivar Kainong 1760 was analyzed. The data of regional trials for small-pod peanut varieties in Henan Province in 2014 and 2015 were performed to evaluate the yield and stability of Kainong 1760 based on GGE biplot, and the visual correlation and path analysis were conduct⁃
    ed by R language. Results showed that yield and stability of Kainong 1760 ranked the first in both 2014 and 2015.Coefficient of variation of shelling percentage was the smallest. The positive correlation coefficient between full pod percentage and yield was extremely significant. And 100-pod weight, single plant productivity, 100-seed weight,main stem height, lateral branch length, branches number with pods had significant positive correlations with yield.The direct path coefficient of branches number with pods was the largest, indicating that it was the key trait affect⁃
    ing yield of Kainong 1760. In conclusion, Kainong 1760 was suitable for large-scale promotion and utilization inHenan Province for its high yield and strong stability. Meanwhile, branch number with pods should be priority consideration during breeding practice of new small-pod peanut cultivars.
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