The additional lines of Brassica napus with one specific chromosome of the medicinal plant songlan （Isatis indigotica） showed particular antiviral activity, but the nutrients, yield of bolts （vegetable parts）, and comprehensive benefits remain to study. In this study, antiviral addition line Songyou 1 （used as paternal plant）, and nuclear sterility line Chuan A-3 （used as maternal plant） were crossed to selected Songyou 2 as experimental material. Yield and quality of seeds and vegetable bolts, the agronomic characteristics, and comprehensive benefits were investigated. Results showed that 48.56% plants presented chromosome-specific SSR fragments of songlan. As vegetables, contents of vitamin C and zinc were higher in bolts than those of broccoli （1-1-5）, cauliflower （rB-112）, B. parachinensis （Q10005）, Chinese kale （Chudong niuxin ganlan） and radish （CP4）. Amino acids and protein contents were higher in bolts than those of cauliflower （rB-112）, B. parachinensis （Q10005）, Chinese kale （Chudong niuxin ganlan） and radish （CP4）, comparable to broccoli （1-1-5）. The average start-cutting days of sprouts were 117 d, with vegetable bolt yield of 16 908.8 kg/hm2, increased by 75.1% compared with oilseed-vegetables cultivation control. Compared with oilseeds cultivation, the final-flowering period was postponed by 6-10 days, and plant height, siliques per plant, seeds per silique, 1000-seed weight, plant yield and oil content were reduced significantly. The predicted biomass at final flowering period was 124.3 t/hm2. The seeds yield was 1127.3 kg/hm2, increased by 16.4% compared with oilseeds-vegetable cultivation control, but decreased by 42.3% compared with the oilseeds cultivation. However, compared with oilseeds cultivation, the average comprehensive benefit of oilseeds-vegetables cultivation was 108 892.4 RMB yuan/hm2, increased by 92 920.4 yuan/hm2. The dual-purpose cultivation for oilseeds and vegetables of Songyou 2 had great sense for both human health and rapeseed cultivation.