Xun-tong MA, JI-Biao, Zhan-zhou MA, Xiao-hui DONG, Si-ning HAN, Liu-yang HAN, LI-Xu, Jia-rui LIU, ZHANG-Yu, Xue-zhen FENG, Xu-hang ZHANG, Ya-qi HUANG, Qing-shan CHEN, Zhao-ming QI, Xiao-xia WU
Soybean seed hardness is related to food and feed processing. For molecular marker-assisted selection of soybean seed hardness, we found related candidate genes, and used chromosome segment substitution lines (CSSL) to localize QTL for soybean seed hardness for two years. Combined with the 25 seed hardness QTL obtained previously, MCScanX was used to analyze the whole soybean genome to generate collinearity block, evaluate the collinearity among the seed hardness QTL, and identify the central QTL located in Gm02 fragment. Multiple databases were used to analyze the genes of the Hub-QTL region and anchor. Eight candidate genes related to seed hardness in soybean. Two lines with different seed hardness and recurrent parents were selected from the CSSL population for subsequent quantitative real-time PCR analysis. It was found that the expression of candidate genes Glyma.02G269400 and Glyma.02G269500 were significantly different in the two strains of CSSL population and the recurrent parent Suinong 14. The relative expression level of Glyma.02G262600 in Suisunong 14 was about 5 times that of CSSL-136, while the expression level of Glyma.02G262600 in CSSL-200 was moderate, suggesting that this gene inhibited the formation of soybean seed coat.