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  • 2014 Volume 36 Issue 2
    Published: 28 April 2014
      

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  • JIA Yan-li, WU Lei, LU Chang-ming*
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    Abstract:In order to improve the components of fatty acids in rapeseed oil, 6 SAD genes of Brassica napus were retrieved by searching putative stearoyl-acyl carrier protein desaturase (SAD) genes from B.rapa genome according to the characteristic SAD genes of Arabidopsis thaliana. The results of sequence alignment showed that the similarity of the six SAD protein sequences of B. napus ranged from 53.2% to 96.3%. Phylogenetic analysis further showed that SAD genes of B. napus had high sequence similarity to those from Ricinus communis, Clycine max and Sasamum indicum etc., indicating that SAD genes of B. napus and other higher plants were highly conserved during evolution. We analyzed the expression patterns of BnSAD1:1, BnSAD2:1, BnSAD2:2 and BnSAD2:3, and found that all the 4 genes were expressed during the seed development, and the relative expression level of 4 genes were the highest at 40DAF (day after flower). It suggested that all these 4 genes involved in the formation of oleoyl-ACP derived from the dehydrogenation of stearoyl-ACP, and BnSAD2:3 gene was likely to express specifically in seed.

  • NA Xiao-fan JIAN Bo,WU Cun-xiang, HOU Wen-sheng, JIANG Bing-jun, HAN Tian-fu
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    Abstract: CONSTANS (CO) acts in the nucleus to promote the transition from vegetative growth to flowering in Arabidopsis, while its homologs are present in many other plant species regardless of the type of photoperiodic response. In this study, we cloned 3 CO homolog genes from a late-maturing (photoperiod-sensitive) soybean cultivar, Zigongdongdou using RLM-RACE. We then identified 5 additional CO homologs by blasting the soybean draft genome (http://www.phytozome.org). The 8 genes all consisted of 2 exons and 1 intron. They had distinctive 3’- and 5’-UTRs from each other, suggesting that their transcriptional and post-transcriptional regulation could be different. In silico analysis found that all the 8 CO homologs contain a highly conserved B-box domain and CCT domain, which indicated that they functioned similarly to AtCO. The identity percentage of predicted amino acid sequence of the 8 CO homologs ranged from 39% for Glyma04g06240 to 51% for Glyma19g05170 and Glyma13g07030, when compared with AtCO. Phylogenetic analysis showed that the 8 CO homologs were classified into 4 clades and each clade had 2 CO homologs with a high degree of amino acid identity. qRT-PCR results showed that 3 CO homologs Glyma04g06240, Glyma13g07030 and Glyma06g06300 had a diurnal rhythm of expression with a peak at dawn (24h) under both short day (SD) and long day (LD) conditions, except that they were lower under LD than under SD. The expression of Glyma13g07030 was up-regulated in leaves 7 days after emergence (DAE) under SD and down-regulated 13 DAE under LD. There was no obvious change for the expression of Glyma06g06300 between LD and SD in leaves. In addition, the transcripts of Glyma13g07030 and Glyma06g06300 were detected in the shoot apical meristem, flower and pod. Our results suggested that the CO homolog Glyma13g01290 might have participated in flowering regulation of Zigongdongdou.

  • XU Kun, LI Feng, WU Jin-feng, GU Tie-cheng, CHEN Bi-yun, GAO Gui-zhen, YAN Gui-xin, LI Jun, QIAO Jiang-wei, WANG Nian, WU Xiao-ming*
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    Abstract: To improve the accuracy of rapeseed cultivars fingerprint detection and to construct a large-scale rapeseed new variety fingerprint databases in the future, a fingerprint identification platform was constructed by capillary electrophoresis detection method with fluorescent SSR markers in the national winter rapeseed varieties (lines) field trials. 42 locus and 131 alleles were detected by 40 fluorescent SSR primers in 163 newly bred winter rapeseed (Brassica napus L.) varieties (lines). 25 locus were detected in A genome (n1-n10) and 17 locus were detected in C genome (n11-n19). Alleles were obtained from 2-6 with average of 3.02 at 42 locus. The polymorphic information content values (PIC) ranged from 0.1 to 0.69, with a mean of 0.36. Primer BRGMS171 was determined according to the heterogosite rate (0.67) and PIC value (0.7) which could be used as preferred core primer for hybrid purity identification. The average SSR locus homozygosity of 18 open lines was 81.9%, and that of 145 hybrid varieties was 57.9%. Dice similarity coefficient among 163 newly bred winter rapeseed varieties (lines) were calculated by 131 alleles, with an average range of 0.607 to 0.765. The maximum variation degree was in FC03 with a ranged of 0.438 - 0.879 and the minimum was in Yiyou21 with a range of 0.611 - 0.806.
    Key words: New winter rapeseed varieties (lines); Fluorescent SSR markers; Capillary electrophoresis

  • XIE Ling, WANG Zhang-xun, HUANG Bo*
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    Abstract: Based on soybean genome database, soybean TPS family genes were identified and their sequences, structures and chromosome location were determined by bioinformatic method. TPS proteins were classified according to their structure and phylogeny relationship. The RNA-sequencing data of TPS family genes from the soybase database was used to analyze the expression pattern of these genes in different tissues and treatments. The results show that a total of 20 TPS genes, located on 14 chromosomes respectively, could be systematically identified from soybean genome and classified into two classes. And all of them had stress related cis-acting elements in their promoter regions. Transcription analysis revealed that there was predominant expression of most soybean TPS family genes in flower, root and nodule. These results will facilitate functional analysis and utilization of TPS genes in soybean.

  • QI Zhao-ming, HOU Meng, HAN Xue, QI Hui-dong, JIANG Hong-wei, XIN Da-wei, ZHU Rong-sheng, HU Zhen-bang, LIU Chun-yan, HU Guo-hua*, CHEN Qing-shan*
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    Abstract:In order to develop the key association loci for soybean oil and protein content, ninety-two soybean cultivars or germplasm of China Northeast area were selected to be analyzed for oil and protein content based on Meta, and to be analyzed and estimated for the population structure based on mathematic model. The loci associated with oil and content were analyzed by employing the general linear model. The results showed that the population was divided into 5 sub-population when K value was 5 by multiple sample test. The oil content associated markers with contribution >1% included Sat_412, Sat_195, Satt317, Sat_187, Sat_195, Satt255, Satt713, Satt468, Satt267, Satt686, Sat_294, AZ302047 and contributed to oil content with a total of 39.54% at p<0.05 significant level. The protein content associated markers included Satt683, Sat_311, Satt578, Satt181, Satt317, Satt700, Satt713, Satt255, Sat_242 and Satt720 with a total of 48.39% contribution. These key loci could be used for marker assisted selections for soybean oil and protein content.

  • LIU Hong-yan, WU Kun, YANG Min-min, ZHOU Xin-an*, ZHAO Ying-zhong*
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    Abstract: Plant hormones play a key role in anther development. With the technology of enzyme-linked immunosorbent assay (ELISA), contents of four endogenous hormones (IAA, ABA, JA and SA) in buds were evaluated at different developmental stages. The ratios of different hormones, as well as contents of soluble sugar and starch were compared among male sterile and fertile plants. The results indicated that: 1) during anther development, the variation trends and contents of four hormones were distinctive between male sterile and fertile plants, and the significant decrease of IAA and increase of ABA in male sterile plant could be connected with the occurrence of male sterility; 2) significant increase of JA and ABA and the decrease of IAA were observed in leaf in male sterile plants; 3) the variation trends and ratios of IAA/ABA, IAA/SA and IAA/JA were different among male sterile and fertile plants, indicating that the hormonal imbalance was also responsible for anther abortion; 4) the soluble sugar and starch contents in buds of male sterile plants were higher than that in fertile plants, which was at least partially responsible for male sterility. 

  • DAI Meng-yuan, AO Tao, XU Wei, LIU Ai-zhong*
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    Abstract: To reveal the potential function of DNA glycosylases mediating DNA demethylation leading to gene imprinting in the growth and development of castor bean (Ricinus communis), the putative genes encoding DNA glycosylases were identified and characterized based on castor bean genome reference data. 3 genes (RcDME, RcROS and RcDML) encoding DNA glycosylases were identified. Their structures, conserved domains, and subcellular localization were characterized. Gene expression was inspected based on the high-throughput transcriptional sequencing data and RT-PCR. Results showed that the amino acids coded by these 3 genes were unstable ones, and the sequences contained typical DNA-binding conserved domains and domains related to base excision repair. Phylogenetically, DME, ROS and DML were homologous and evolved independently in plants. Both RcDME and RcROS exhibited a similar expression pattern among different tissues with the highest expression in endosperm, while the expression level of RcDML was not detected in all tested tissues.

  • YUAN Jin-zhan, MA Ni, ZHANG Chun-lei*, LI Jun
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    Abstract: To understand root regulation on rapeseed yield by easy and reduced cultivation technique, a field experiment was conducted to investigate the distribution of root population under two cultivation methods and two nitrogen fertilization levels. The cultivation methods were direct drilling at 450 000 plants per hectare, and transplanting at 120 000 plants per hectare. The two fertilization levels included pure nitrogen of 180kg per hectare and 270kg per hectare. Cultivar Zhongshuang 11 and Zhongyouza 12 were used as plant materials. Results showed that the root distribution of direct drilling was deeper. It had significant fewer roots at 0-10cm soil layer, and significant greater distribution at both 10-20cm and 20-30cm layers. At initial flowering stage, root dry weight was the most important factor on population yield, and the heavier the dry weight, the higher the yield. Strong root system was also the key factor on yield. Roots grew stronger under transplanting method, and higher nitrogen rate benefited a stronger root system.

  • SUN Li, WAN Yong-shan*,LIU Feng-zhen, SUN Ai-qing, ZHANG Kun
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    Abstract: A method based on sand culture and 20% polyethylene glycol-6000 (PEG 6000) was developed to study the dynamic variation on flavonoids and related enzymes activity in leaves of four peanut varieties (Hua 17, Nongda 818, Fenghua 5, Shanhua 11) seedling with simulate water stress. Results showed that the contents of flavonoids in all varieties seedling leaves decreased initially and increased subsequently,and peaked after 9h under 20% PEG stress, Nongda 818 with Fenghua 5 and Shanhua 11 decreased to control levels at 72h after the treatment, hua17 was about 36h. The activities enzymes of PAL with CHS and CHI gradually increased after PEG stress, CHS and CHI activities peaked at 6h, PAL activity peaked at 9h, then activities declined. These 4 peanut varieties had the same trend. Analysis showed that the reasons of quick synthesis in large quantity for flavonoids of Shanhua 11 and Fenghua 5 for were their higher CHS activity and accumulation rate. PAL of Nongda 818 had lower activity and accumulation rate which limited accumulation of flavonoids. The lower activities and accumulation rates of these 3 enzymes on Hua 17 resulted slow synthesis and small quantity of flavonoids. The correlation analysis indicated that peanut flavonoids was regulated by the combined effect of PAL, CHS and CHI, and the variances of flavonoid contents were a result of changes of these enzymes’ activities under PEG stress.

  • WANG Zhu1, YANG Wen-yu2*
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    Abstract: Field experiments were conducted to determine the optimal field combination that benefited soybean carbon-nitrogen metabolism and yield in “maize/ soybean” relay-cropping system. Split plot design was adopted, with different plant-types of maize as main plots, planting width as sub-plots. The results showed that under large width and relay cropping with erect maize, shade damage to soybean was reduced. The trend of soybean carbon-nitrogen metabolism was beneficial to high yield. The content of carbon and nitrogen and the activity of SPS and GS kept at a high level at every growing stage. After R4, the starch in leaf degraded into sugar quickly and transferred to grain, and the decreasing rate of nitrogen slowed down. Basis on our previous research work, the result suggested that R2 was the crucial stage for soybean to experience light change (maize harvest time was around R2), so the carbon-nitrogen metabolism ability of soybean at R2 had a strong impact for yield. And the carbon-nitrogen metabolism levels of soybean at R6 could partly reflect yield. In conclusion, relay-cropping soybean with erect maize in planting width 1.17m / 0.83m (soybean/maize) was the best field combination that could ensure high yield and efficiency all year round.

  • CAO Jin-hua, ZHU Jia-cheng*, ZHANG Shu-fen, WANG Jian-ping, WEN Yan-cheng, ZHAO Lei, HE Jun-ping
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    Abstract: To understand the effect of covering on soil temperature, rapeseed cold resistance and yield in Huang-Huai Area, Brassica napus L. cv Fengyou 10 was used in field experiments during 2009 and 2012. Two coverings as straw cover on surface and soil cover on root crown were designed before and after winter. Temperature at different soil layers, cold damage level, economic traits, yield and quality were investigated, with no covering as control. Results showed that straw cover before winter significantly improved the soil temperature and reduced the freezing index, and consequently improved survival rate. Freezing index under both straw-covering treatments were significantly lower than control, and yields were significantly higher (with 1.55% to 58.28% increase) than control, especially with the covering applied before winter. The yield increase was due to significant increase of effective pods number and thousand-seed weight. Their increases were 4.48% to 20.39%, and 29.97% to 41.11% respectively. No significant change of seed quality was found. This study proved that covering both with straw and soil could benefit the rapeseed yield and cold resistance at Huang-Huai Area, especially before winter.

  • LIU Wei-guo,ZOU Jun-lin,YUAN Jin, JIANG Tao, WU Xiao-ling,YANG Wen-yu*
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    Abstract: The main agronomic characteristics and yield of 33 soybean materials were studied under relay intercropping systems. The results showed that the average height of materials reached 122.98cm with relatively less node number and prolonged node length. Branches were extremely important to yield formation, the average branch number of materials was 5.05, while branch yield accounting for 81.1% of the plant yield. Correlation analysis showed that the yield of relay intercropping soybean was significantly positively correlated with the seed weight of branch, the height of the highest branch, the effective branch numbers, the average branch length, the number of node on main stem, the growth period and vegetative period, and the length of vegetative period. The strong capability of branching and bearing were essential for higher yield in relay intercropping soybean. Principal component analysis showed that the branching factor, stem factor, yield factor and pod factor altogether contribute 77.28% to the variation rate. Results of this study will provide theoretical basis for selection and breeding of special soybean varieties for relay intercropping planting.

  • WANG Qiang, ZHAO Li, ZHANG Zi-fu, TIAN Dong-feng, HANG Xin-fen, LIN Yong-xiang
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    Abstract: According to the technical difficulties of sesame mechanical planting, based on the idea of the combination of agricultural machinery and agronomy, the information collection of sesame mechanical planting was carried out in China. 7 sets of medium and small-sized practical mechanical equipment suitable for sesame planting were introduced, selected and reformed, and key technologies of sesame production mechanization were proposed. The results of mechanical planting showed that suitable row spacing of sesame machine sowing was 33.3-40.0cm, appropriate seeding rate was 0.3 kg/666.7m2. The practical herbicide was 99% acetochlor before seedling after sowing. The highest yield was 151.0 kg/666.7m2, which occurred in the treatment of no-tillage, machine sowing and base application of compound fertilizer 25 kg/666.7m2 and topdressing urea 5 kg/666.7m2. The yield increased 16.25% with exempting tillage, machine sowing and straw returning treatment, compared with no-straw returning treatment. The yield of chemical thinning and singling was close to the artificial thinning and singling, but the chemical thinning and final singling could save the labor cost more than 100 yuan/666.7m2 and could effectively control the weeds at seedling and seedling shortage. Nine times sesame mechanical planting and high-yield demonstration were carried out between 2009 and 2012. The average yield was 116.3kg/666.7m2 and increased by 34.2% compared with the non-demonstration areas.

  • SONG Li, HAN Shang, XI Ying-ying, LU Jian-wei, WU Li-shu, CAO Wei-dong, GENG Ming-jian
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    Abstract: For sustainable land use and higher revenue on both green manure and oil seed rape (Brassica napus L.), a practical rape/Chinese milk vetch (Astragalus sinicus L.) intercropping system was to be established without affecting yields of rapeseed. Effects of the intercropping (planting two lines of rape with one line of Chinese milk vetch within one meter width) under different planting densities (from 200 000 to 400 000 plants per hectare) of rapeseed were investigated on rape growth and yield in field. Results showed that the accumulation of nitrogen, phosphorus, potassium and carbon in rape were close to mono-cropping rape (MR, at the density of 300 000 plants per hectare) or higher. The plant height, root length, root crown diameter, weight per plant and yield were similar to MR or significantly higher. About 3 592 to 5 724 kg per hectare of green manures were harvested when rape yield maintained at the MR level. Thus, the system showed clear advantage of land resources with LER (land equivalent ratio) more than 1.
    Key words: Rape; Chinese milk vetch; Intercropping; Growth and development; Land equivalent ratio; Yield

  • ZHANG Jin-hua, GUO Sheng-jin,WANG Xin,GUO Ping-yi*
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    Abstract: Perilla with six leaves was exposed to combined treatment with urea (0, 1, 2, 4 and 8g/L) and puma super (0, 1.33, 2.66, 5.32 and 7.98mL/L) of different concentration to study the mitigative effect of urea on Perilla frutescens (L.) Britt. seedlings to Puma super stress and to find the best treatment. The effect of combinedd treatment on photosynthesis, chlorophyll fluorescence, weed control and seed yield of Perilla frutescens (L.) Britt. were studied by using the randomized block design experiment. Results showed that the chlorophyll content index reduced significantly, and photosynthetic rate (Pn), stomatal conductance(Gs) and transpiration rate (Tr) also reduced at various levels. The intercellular CO2 concentration (Ci) increased under Puma super treatments. It indicated that Puma super significantly inhibited the photosynthetic capacity. It also showed that Puma super significantly decreased maximum PSII photochemical efficiency, effective quantum yield of photochemical energy conversion in PSII, PSII potential activity and photochemical quenching of variable Chl fluorescence; and decreased non-photochemical Chl fluorescence quenching. It indicated that Puma super impaired PSII and blocked the electron transport in light reaction, treatment with urea of moderate concentration (1 to 4g/L) could antagonize the negative effect contributed by Puma super, while high concentration (8g/L) could aggravate this effect. The seed yield and weed control efficiency increased 11% and 11.35% respectively under leaf spray of 4g/L urea and followed by leaf spray of 1.33mL/L Puma super. These results indicated that the mitigative effect of urea on Perilla frutescens (L.) Britt. seedlings to Puma super stress increased effectively. The photosynthesis and PSII photochemical efficiency were improved after treated by 4g/L urea first and then by 1.33 mL/L Puma super. This combination of urea and clethodium could balance the fertilization and weeding, and further promote crop production. 

  • HAN Guang-zhen, HAN Fen-xia*, SUN Jun-ming, YU Shao-xuan, ZHANG Jin-wei, YAN Shu-rong, YANG Hua
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    Abstract: Sclerotinia stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary has a wide host range. Genetic diversity of 32 isolates of S. sclerotiorum collected from different geographical regions and host plants were detected by SSR markers. A total of 69 alleles were observed using 17 pairs of primers with an average of 4.06 alleles for each pair of primers. The alleles with the largest numbers (8) were found in 2 pairs of primers (AF377908-1/AF377908-2 and AF377922-1/AF377922-2),and the least number of alleles (2) occurred in the other 2 primer pairs (AF377903-1/ AF377903-2 and AF377925-1/AF377925-2). The mean value of DNA polymorphic information content (PIC) was 0.56. The highest PIC value was 0.78 detected with AF377922-1/AF377922-2. In cluster analysis, 32 isolates were divided into 5 groups. Most isolates from the same region were grouped in the same or a close population, suggesting that Sclerotinia genetic loci within populations were relatively consistent but significantly different among populations. 

  • SHIShu-sen,CUIJuan,XUWei,WUTing-ting,QILing-zi, LIWei-yu,SONGPeng-xiang,ZHANGMeng
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    Inordertoexploretheeffectsoftemperatureondevelopmentofthesoybeanpodborer,Leguminivo
    raglycinivorella(Lepidoptera:Olethreutidae),thedevelopmentaldurationandsurvivorshipofthispestwereinvesti
    gatedinthelaboratoryat17,21,25,29and33℃.Theresultsshowedthatthedevelopmentalrateofeggsincreased
    withtheincreasesoftemperaturefrom17℃ to33℃.Therelationshipbetweendevelopmentalrateandtemperature
    couldbefittedwellwithLogisticmodel:Yegg=0.61/[1+exp(3.19-0.08t)](F=128.5976,p=0.0077,R
    =0.9961).Developmentalrateoflarvaincreasedwiththeincreasesoftemperaturebelow33℃.The2ndand3rd
    instarlarvagrewfasterthantheotherinstars.Whiledevelopmentalrateofthe1st,4thand5thinstarlarvade
    creasedsharplyat33℃.Therelationshipbetweendevelopmentalrateoflarvaandtemperaturecouldbedescribed
    byLogisticmodel:Ylarva=0.10/[1+exp(3.25-0.17t)](F=21.7316,p=0.0440,R=0.9778).Egg
    hatchingratewasinsignificantlyinfluencedbytemperature,butthesurvivalrateoflarvawassignificantlyinflu
    encedbytemperature.Theadaptabilityoflarvatoenvironmenttemperaturegraduallyincreasedwiththeinstar.
    Temperatureaffectedthelarvalnutritionaccumulation.Thematurelarvabodyweightwas17.26mgat25℃ which
    wassignificantlyheavierthanatothertemperature.Thedevelopmentthresholdtemperatureandtheeffectiveaccu
    mulatedtemperaturewere7.24℃,117.63d·℃ foreggsand4.71℃,285.33d·℃ forlarva.

  • LI Jing,LI Pei-wu*,ZHANG Qi*,DING Xiao-xia,ZHANG Wen,ZHANG Zhao-wei
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    Abstract: This research used time-resolved fluoro-immunochromatographic assay strip and assorted time-resolved fluoroimmunoassay (TRFIA) portable quick tester to detect aflatoxin B1 in oilseed meals. The TRFIA technology was based on the sample bottle which contained specific monoclonal antibody marked by Eu (Ⅲ) and the time-resolved fluoro-immunochromatographic assay strip. The standard curve could be built in the TRFIA portable quick tester and the machine could output the results directly. In the standard addition experiments of six kinds of oilseed meals, the recovery rate of standard addition was 70%-120% (CV<15%). In the determination of practical samples, compared with LC-MS/MS method, the relative error of the time-resolved fluoro-immunochromatographic assay strip method was less than 15%. The results showed that the new method was fast, accurate, stable and reliable, and the instrument was cheap and small-sized. Because of these advantages, time-resolved fluoro-immunochromatographic assay strip could be used for aflatoxin B1 screening in oilseed meals and risk assessment, and the application prospect would be very broad.

  • YANG Mei, LI Wen-lin, LIU Chang-sheng, ZHOU Qi, ZHENG Chang, HUANG Feng-hong*
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    Abstract: Microwave pretreatment increases the oil extraction yield from rapeseed, but still some oils remained in the press-cake. To compare the physicochemical quality of press oil and the solvent-extracted oil from press-cake, rapeseeds with moisture of 12% were treated with microwaves under 800W for 1 to 7 min at frequency of 2 450MHz, and pressed oil from cold seeds, as well as extracted from the press-cake by ultrasonic assisted n-hexane solvents. Results indicated that microwave time and oil extraction method (by press and by solvent) had significant effects on acid values (AVs), peroxide values (POVs), p-anisidine values (p-AnVs), and moisture contents of the two oils (P<0.05). The AVs and p-AnVs of two oils increased with increasing microwave time, and the POVs increased initially and then decreased depending on the time of microwave radiation. The AVs, POVs, and p-AnVs of pressed oils were higher than those of solvent-extracted. The moisture contents of oils extracted by press were higher, and would increase with the increasing microwave time, while the extracted oil decreased. The Lovibond color of extracted oil was darker, and the longer the pretreat time, the deeper color of both oils. No precipitates were found in pressed oils after heating (at 280℃) test, while a lot of precipitates were produced in the extracts. In conclusion, pressed oil pretreated by microwaves only needed to be purified by washing, filtering or centrifuging before consumption, while the solvent-extracted oil must be refined by degumming, deacidification and bleaching.

  • ZHAN Shi-xiong, ZHENG Yi-xiong, LIU Guan-ming, ZHANG Ping-hu, YANG Ling, ZHUANG Dong-hong*,
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    Abstract: In this study, 48 SSR markers were used to assess the genetic variation of 63 peanut cultivars. The results showed that total 251 polymorphic alleles were detected and the number of polymorphic fragments per marker ranged from 2 to 13 with an average of 5.23. The genetic diversity index ranged from 0.508 to 2.243 with an average of 1.272. Polymorphism information content (PIC) ranged from 0.252 to 0.873 with an average of 0.647 per primer pairs. Genetic similarity coefficients (GS) ranged from 0.657 to 0.960, GS were lower among different botanical types, and genetic relationship was more distant among different origins. Cluster analysis was performed with the GS and the UPGMA method. 63 cultivars were divided into 4 groups at the threshold of 0.74. The result of clustering analysis supported the conventional classification.

  • WANG Xing,LIU Xiao-fei, ZHOU Yi-jun, ZHANG Gen-fa
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    Abstract: SOD is one of the most important metal-chelated enzymes in active oxygen-scavenging system, and plays a pivotal role in clearing O2- .. SOD regulation is a complicated process, including transcription factors regulation in transcriptional level, microRNAs in posttranscriptional and translational levels. This review introduced structures and functions of SOD, molecular mechanism of transcriptional and posttranscriptional regulation of SOD genes and relationship of SOD with plant abiotic resistance.

  • WANG An-ke, SUN Ying-chao, HE Qiu-ling, PAN Jing-jing, LI Shu-xia, ZHU Shui-jin, CHEN Jin-hong*
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     Abstract: Acyl-CoA: diacylglycerol acyltransferase (DGAT) is the only key enzyme evolved in the Kennedy pathway and catalyzes the last step. This paper summrized the features of DGAT1 (subtype 1 of DGAT family) in eukaryote, including construction, substrate specificity and spatio-temporal expressions. It especially reviewed DGAT1 function on plant growth and development. In discussion, some suggestions were made for further study of DGAT1 in plant.