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  • 2015 Volume 37 Issue 5
    Published: 28 October 2015
      

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  • SUN Yan-yan,QU Gao-ping,HUANG Qian-xin, LV Jin-yang,GUO Yuan,HU Sheng-wu *
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    For molecular markers for tribenuron-methyl (TBM) resistant mutant in Brassica napus L., M2 population of rapeseed (B. napus) cv Zhongshuang 9 mutagenized by ethylmethane sulfonate were treated by foliar spraying of TBM solution. 3 TBM-resistant mutants, K1, K4, and K5, were screened out from ~30 000 individuals of M2 population. BnALS1, BnALS2 and BnALS3 genes of these TBM resistant mutants were cloned and detected for single nucleate polymorphisms (SNPs). Results showed that BnALS3 gene from K1 and K4 had C to T transition at position +535 which led to substitution of proline with serine at position 197 in BnALS3 protein. K5 BnALS1 also had C to T transition at position +544 which led to substitution of proline with serine at position 197 in BnALS1 protein. This BnALS1: Pro197Ser mutant in K5 was first reported in B. napus. According to allele-specific polymerase chain reaction (AS-PCR) results, 6 primers could identify these mutants from wild type. Among them, 2 primer pairs were used for SNP of K5 BnALS1, 4 for K1 and K4 mutant gene BnALS3.

  • LI Qian-bo, YANG Chun-yan§, HUANG Li, REN Xiao-ping, WANG Hou-miao, CHEN Yu-ning, ZHOU Xiao-jing, JIANG Hui-fang, LEI Yong, YAN Li-ying, LIAO Bo-shou*
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    To create novel peanut germplasm and develop Marker-Assisted Selection (MAS) techniques for aflatoxin resistance in peanut, recombinant inbred line (RIL) population derived from Zhonghua 10 and ICG12625 with different levels of resistance to Aspergillus flavus was tested for reaction to fungi infection and aflatoxin production by artificial inoculation. Results of genetic analysis showed that the 2 resistance components were controlled by 2 mixed major genes and polygenes. One RIL line(QT401)with low infection index and 4 RIL lines including QT344, QT389, QT477 and QT483 with low aflatoxin production were identified through resistance evaluation. A peanut genetic linkage map covering 1 165.45 cM with 20 linkage groups, was constructed by using Joinmap 3.0, and a total of 458 SSR loci were anchored on this map. Furthermore, composite interval mapping (CIM) was implemented by using WincartQTL 2.0. 6 QTLs for resistance to seed invasion of A. flavus and 10 for resistance to aflatoxin production were identified, explaining 6.34% to12.00% phenotypic variation respectively.

  • FAN Hui-ling, BAI Sheng-wen, CHANG Li-guo, SUN Wan-cang*
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    To investigate the genetic variation of self-compatibility within Eruca sativa Mill., self-compatible lines were selected to maintain genetic purity and produce hybrid seeds. The heterosis of these hybrid seeds was then studied. The self-compatible variations of the major E. sativa materials from China and 6 materials from other countries, were examined by monitoring the seeds in self-pollinated and isolated flowers in field conditions. The results showed that the tested cultivars could be divided into 4 groups, which were highly self-incompatible, self-incompatible, self-compatible, and highly self-compatible. In self-incompatible cultivars, the mean compatibility index (CI) ranged between 0-4.98. The cultivars were considered self-compatible when CI was more than 1. Among all the studied cultivars, 3 were highly self-compatible, which were Sichuan-2 Yunjie, 06yun86-y, and 06yun86-87, 6 were self-compatible and 43 were self-incompatible. Moreover, significant variation among different individual plants was observed, and the percentage of self-compatible plants increased with the increasing self-compatibility of the cultivar. In summary, E. sativa is a highly self-incompatible plant with varied self-compatibility. Thus, self-compatible lines could be selected and utilized in rapeseed breeding and genetic studies. 

  • CAO Hui-hui, HUANG Jun-yan, KE Tao, YU Jing-yin, DONG Cai-hua*, LIU Sheng-yi*
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    In order to analysis resistant ability of Rs to Sclerotinia sclerotiorum, Rs was artificial synthesized and combined with a rice α-Amylase signal peptide αAmy3SP which can cargo proteins to plastids and intercellular space of plant cells to construct plant expression vector 35S-SP-Rs and 35S-SP-GFP. Gene gun bombardment onion experiment showed that fluorescent signal of GFP existed in the extracellular matrix between cell wall and membrane. This result illustrated that GFP was effectively transported as cargo into extracellular matrix under the guide of 35S and αAmy3SP. Rs gene was introduced into Arabidopsis via Agrobacterium tumefaciens-mediated transformation. 35 positive p35S-SP-Rs transgenic plantlets were obtained by PCR assay and kanamycin selection. Relative expression level of Rs in transgenic Arabidopsis was much higher than that of WT lines as detected by qRT-PCR with normal growth and development. Disease resistance identification showed that Rs transgenic plants had higher resistance to S. Sclerotiorum than non-transgenic plants. This study showed that the presence of high amount of antimicrobial peptide Rs in extracellular matrix could reduce cytotoxicity and enhance resistance of plants to S. sclerotiorum by significantly inhibiting extension of fungal hyphae. 

  • LIN Mei-jing, ZHANG Li-wei, ZHANG Hui, WANG Jin-hui, LIN Meng-meng, YU Guo-long, YU Ren-jing, LIU Chun-yan*, CHEN Qing-shan*
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    Sumoylation played an important role in pathogen defense of soybean. By bioinformatics method, 6 SUMO related genes were obtained from soybean. Expression of these genes were analyzed at transcriptional level by real-time PCR after inoculated with Pseudomonas syringae pv. glycinea in resistant variety HN37 and susceptive variety HF25. Results showed that, in HN37 leaf, expressions of GmSUMO2 and GmSCEa were the highest at 24h, GmSUMO3 and GmSCEb were at 48h. Therefore GmSUMO2/3 and GmSCEa/b might have played a major role in leaf disease-resistance process. Expression of GmSAE2a at 48h was 6-fold higher than at 0h which might have played a major role in stem disease-resistance. Expression of GmESD4e decreased significantly after inoculation, which might have played a major role of desumoylation in HN37. Expression of GmESD4a/e was the highest at 24h in leaf and at 72h in root of HF25 which might have played a major role of desumoylation of HF25. In summary, the soybean SUMO system was associated with resistance to P. syringae pv. glycinea.

  • QU Jing, WANG Pi-wu*,GUAN Shu-yan, FAN Yu-guang, LIU Si-yan, YAO Dan, LI Xia
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    In order to provide better soybean forage for young animal, RNAi expression vector of soybean β-conglycinin α′ and β-subunits bivalent gene was built to decrease antigen quantity of soybean protein. Based on RNAi expression vector pCAMBIA3301-PFNZ-BADH, fracture of sense and anti-sense of β subunits gene was linked to each side of fracture of sense and anti-sense of α′ subunits on pCAMBIA3301-PFNZ-BADH by digestion. Then the vector was transformed into soybeans cotyledonary node via Agrobacterium tumefaciens-mediated method. Putative transgenic soybean were analyzed by PCR. 11 transgenic soybean plants were obtained. Southern blot of T1 plants showed that the β-conglycinin α′and β-subunits bivalent gene was successfully integrated into soybean genome. qRT-PCR in soybean seed showed that β-conglycinin α′ subunit and β subunit were both decreased significantly.

  • MA Li-gong,MENG Qing-lin,ZHANG Yun-hua*,LIU Zhi-hua,WANG Zhi-ying*
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    To explore the disease resistance role of a glutathione S-transferases (GST) gene in sunflower, a full-length cDNA of GST (LSK-2) was cloned based on the transcriptome of Helianthus annuus induced by Sclerotinia sclerotiorum. Results showed that the cDNA contained a 675bp open reading frame (ORF) encoding 224 amino acids. The predicted protein was 55.45 kDa with isoelectric point of 5.16. This gene was named as HaGSTU1 (GenBank accession No. KR071872). HaGSTU1 protein contained Tau GST-specific N-terminal domain (G site) and C-terminal domain (H site). HaGSTU1 was predicted to be a cytoplasmic protein which shared the highest amino acid identity (72%) with a GST from Hevea brasiliensis. qRT-PCR results showed that HaGSTU1 was highly expressed in leaf, and less in sunflower head. Its expression was induced by drought, salt, oxalic acid, S. sclerotiorum and its metabolites. Then the HaGSTU1 gene was transformed into tobacco by Agrobacterium tumefaciens to further verifying its function. The results showed that the expression of HaGSTU1 improved the resistance of transgenic lines to S. sclerotiorum, significantly increased GST and GPX enzyme activity which were about 2 fold of those under S. sclerotiorum infection. It suggested that HaGSTU1 gene conferred a resistance to S. sclerotiorum.

  • HAO Guo-cun,YANG Min-min§, LIU Hong-yan, ZHOU Fang, WU Kun, ZUO Yang, ZHAO Ying-zhong*
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    To develop a simple and convenient marker for genetic linkage of sesame male sterile gene, based on an AFLP marker, 7 SCAR (sequence characterized amplified region) markers were developed using recessive genic male sterile two-type line 95ms-5AB as fertile and sterile gene bulks for target band screening. These 7 markers could detect bands from fertile bulk with 806bp to 1 638bp in size, and could not from sterile bulk. They were designated as PMC-1to PMC-7. These markers were used to detect 1 184 fertile and sterile plants of 95ms-5AB with 0.25% exchange rate. 4 F2 populations obtained from 95ms-5A and different genotypes and 2 recessive genic male sterile lines were identified using these 7 SCAR markers. Result indicated that the SCAR markers were specific for 95ms-5 sesame. 

  • LIU Ya-wen, PAN He, YAO Wen-xin, SUN Xin*, DU Jun-bo, YANG Wen-yu
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    To understand the effect of plastid terminal oxidase (PTOX) in soybean under drought, PTOX gene was studied. 2 essential components of CET (cyclic electron transport), NDH-H (H-subunit of reduced nicotinamide adenine di (tri)-nucleotide phosphate [NAD(P)H] dehydrogenase complex) and PGR5 ( the proton gradient regulation ) were observed in leaves of pot-cultured soybean seedlings. Results showed that PTOX expression was up-regulated obviously under drought, and PGR5 was also up-regulated, while NDH-H remained unchanged. Simultaneously, transpiration rate, stomatal conduction, intercellular CO2 concentration, net photosynthetic rate and chlorophyll content of leaves decreased obviously, while carotenoid content increased, indicating a significant influence of drought on photosynthesis of soybean. Results suggested the up-regulation of PTOX and PGR5 expression were soybean responses to drought. Enhanced PGR5-dependent CET and terminal oxidation of plastoquinol could protect photosynthetic electron transport chain from over-reduction. In addition, increased carotenoid content might improve antioxidant capacity, which was beneficial for soybean under drought.

  • CHEN Si-yu, LIU Peng, ZHU Mo, XU Ke-zhang, LI Da-yong, CHEN Zhan-yu, ZHANG Zhi-an*
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    To better understand the changes of cotyledon physiology of soybean seed harvested from different canopy positions during soybean seed germination, canopies from 2 cultivars Jinong 15 and Jinong 24 were divided into 5 sections according to plant weight. The 5 sections were named lower, lower-middle, middle, upper-middle and upper. Soluble protein, soluble sugar, MDA content, absolute conductivity and antioxidant enzyme activities of cotyledon during germination were detected. Seed yield derived from different sections were analyzed. Results showed that, from bottom to top of these 5 sections, content of soluble protein, soluble sugar and MDA in cotyledons increased gradually. POD activities were high at the 1st section, and then decreased. Absolute conductivity, SOD activities and CAT activities decreased gradually. Seed number, pod number and yield increased gradually. 100-seed weight at the upper section had the least value. It indicated that the lower section and the lower-middle section were the position for their fulfilled seeds which had vigorous metabolism in cotyledons with delayed senescence.

  • ZOU Jun-lin, LIU Wei-guo*, YUAN Jin1, LUO Ling, JIANG Tao, DENG Yu-chuan, CHEN Xue-fei, YANG Chen-yu, YANG Wen-yu*
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     In order to explore the influence of marginal effect on soybean phenotype and yield, 3 soybean cultivars with different podding habits were planted under monocropping and maize-soybean relay strip intercropping pattern. Results show that relay strip intercropping effects positively to main stem length, average internode length, but negatively to node number, stem diameter, above-ground biomass (stem dry weight) of soybean at seedling stage and maturity stage. Effective branch number, main stem pod, branch pod, seed per plant, 100-seed weight, yield per plant of relay strip intercropping soybean were affected negatively by the result of comprehensive marginal effect (marginal disadvantage). In maize-soybean relay strip intercropping, the larger the average internode length, pods of main stem and marginal effect index there were, the stronger the phenotypic plasticity they showed. In terms of cultivars, phenotypic plasticity and marginal effect of inderminate soybean were more significant than those in semideterminate and determinate soybean. Correlation analysis show that marginal effect index of yield per plant was significantly negatively correlated to marginal effect index of average internode length, significantly positively correlated to marginal effect index of aboveground biomass at seedling stage, effective branches, pods of branch, and seed number per plant. Marginal advantage after maize harvested could hardly make up marginal disadvantage during symbiotic period in maize-soybean relay strip intercropping pattern. Therefore, soybean cultivars with weaker phenotypic plasticity and less responsive to marginal effect could improve soybean yield under relay strip intercropping.

  • TANG Jiang-hua, SU Li-li, LI Ya-jie, XU Wen-xiu*, PENG Jiang-long, ZHANG Yong-qiang, HAO Wei-wei
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     To explore high yield tillage method for soybean following wheat harvest in northern Xinjiang, response of soybean to 4 different tillage methods were studied in field experiment. Results of 2-years experiments showed that population leaf area index (LAI) at each growth period were: plowing (T) > rotary tillage (RT) > no tillage (NT). The average of LAI in the whole growth period was 27.25% higher than that of NT in 2 years. Besides, LAI of the whole growth period was also higher than the average of RT and NT after increasing tillage plus film covering (TP) in 2013, and 11.67% higher than T in the same year and reached highly significant difference level (p≤0.01), which greatly enhanced photosynthesis and improve dry matter accumulation. Pods per plant, grain number per plant, grain weight and yield of T treatment were 23.18%, 19.69%, 8.89% and 13.35% higher than those of NT respectively. Net income increased by 32.11%. Especially in 2013, pods per plant, grain number per plant, grain weight and yield of TP treatment were higher than those of T treatment by 28.00%, 27.46%, 2.72% and 7.43% respectively. The difference was highly significant (p≤0.01), resulted in a 12.15% higher net income. In summary, combining plowing with plastic film mulching could be beneficial for soybean crop following wheat harvest under drip irrigation in northern Xinjiang. 

  • JIANG Shi-yang,LI Dong-hua,ZHANG Yan-xin,WEI Xin,ZHANG Xiu-rong,WANG Lin-hai*
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     To explore the phytohormone and phenotype responses to waterlogging stress in sesame, tolerant ZZM2541 and susceptible Ezhi 2 were treated with waterlogging for 3h, 9h and 15h at flowering stage. By liquid chromatography-tandem mass spectrometry (LC-MS/MS), the top 3rd leaves and stem tips of these 2 genotypes were investigated for indole acetic acid (IAA) and abscisic acid (ABA) contents at each time point and 20h after drainage. Phenotype changes were analyzed including shedding leaves and plant heights, and the contents of chlorophyll in the top 3rd leaves before and 72h after the initial of waterlogging. Results showed endogenous IAA contents in the top 3rd leaves were both increased during the first 9h of waterlogging, and started to decrease after 9h. But the contents were continued to increase in stem tips after 9h of waterlogging. Especially, IAA content in ZZM2541 was always higher than that of Ezhi 2 throughout the stress. The contents of ABA in both genotypes decreased continuously until 15h of waterlogging and then increased, although they increased more rapidly in the tolerant genotype ZZM2541 and reached about twice of the susceptible one after drainage. Relatively, the plant height and chlorophyll content in the top 3rd leaves (with more leaf abscission) of tolerant ZZM2541 were less affected by waterlogging stress,which could be a protective mechanism. In summary, IAA content at the initial stage of waterlogging (within 9h ) and ABA at 20h after drainage could be used to differentiate the above 2 genotypes.

  • GUO Peng-hui, GAO Dan-dan, TIAN Xiao-jing, ZANG Rong-xin, LI Gui-hua
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    Physiological characteristics of seed germination and seedlings of Perilla frutescens under polyethylene glycol 6000 (PEG-6000) as drought stress stimulation was investigated. 3 treatments of PEG-6000 concentrations of light drought stress (2.5%, 5.0% and 10%), medium drought stress (15% and 20%) and heavy drought stress (25%) were used to soak the seeds and seedlings. Results showed that the Gr (germination rate), Gi (germination index), Vi (germination vigor index), Gp (germination potential) and Vr (vitality rate) of seeds decreased gradually along with the increment of PEG-6000 concentrations, but there was no significant difference in light drought stress (P>0.05). Gr was significantly decreased in medium drought stress (P<0.05). In heavy drought stress seeds did not germinate. Along with the increment of drought, stem length and fresh weight were markedly decreased, while the main root length increased initially and then decreased. Activity of SOD, POD and CAT also increased initially and then decreased, but MDA content increased continuously. These finding indicated that seed germination and seedling growth of P. frutescens were significantly inhibited by drought stress. Meanwhile the seedlings demonstrated certain adaptability to drought stress via physiological changes.

  • PAN Yong-hui, LU Zhi-feng, LU Jian-wei, LI Kai-xu, LI Xiao-kun, CONG Ri-huan, REN Tao*
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     To understand leaf photosynthesis characteristics and its response to K starvation in oilseed rape, a field experiment was carried out on K-deficient soil with K deficiency (–K) and K supply (+K) treatments. Gas exchange, chlorophyll fluorescence and chloroplast ultrastructure were studied using winter oilseed rape cultivar Zheyou 601 during over-wintering period. Results showed that leaf net photosynthetic rate (Pn) decreased by 45.1% under –K condition. Although stomatal conductance (gs) was lower in –K treatment than in +K treatment, intercellular CO2 concentration (Ci) increased 66 μmol CO2·mol-1 . Limiting value of stomata (Ls) decreased by 45.7%. Thus the stomatal factor changes were not the main reason for Pn decrease under –K condition. Further evidences from chlorophyll fluorescence measurement showed that both photosystem II (PSII) maximum photochemical quantum yield (Fv/Fm) and PSII actual photochemical quantum yield (ΦPSII) were declined under -K treatment, which indicated that PSII reaction center was damaged. In addition, K deficiency affected chloroplast ultrastructure of leaves. In K deficient plants, chloroplast length decreased significantly (with the average of 15.6%). Chloroplast thickness and distance from chloroplast to cell wall increased by 26.9% and 56.1%, respectively. In conclusion, the non-stomatal factors, e.g. damaged PSII reaction center and chloroplast ultrastructure alteration inhibited leaf Pn under K deficiency.

  • CUI Hong-yan, HU Fa-long§, FANG Zi-sen*, REN Pan-rong, NIU Jun-yi
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    In order to identify the effect of different nitrogen level on root morphology and nitrogen utilization efficiency of oil flax, a soil column experiment was conducted with 4 pure nitrogen application rate: without nitrogen (N0), low nitrogen (N1, 78.75kg·hm-2), medium nitrogen (N2, 105kg·hm-2) and high nitrogen (N3, 131.25kg·hm-2). Oil flax cultivar Longyaza1 was used. Results showed that root growth was inhabited by nitrogen application in fir shaped period. However, root length, diameter, surface area and volume initially were raised by the increase of nitrogen, and decreased under N2 after squaring. Root distribution under 40cm-layer soil was increased under N2. Shallow roots increased under higher nitrogen (N3) application in late growth stage. Root-shoot ratio was gradually decreased along with the growth, but was significantly improved under N2 in late period. Under N2 treatment, grain yield reached the highest. After taking into account of grain yield and nitrogen utilization, the nitrogen fertilizer rate of 105kg·hm-2 was recommended.

  • ZHENG Lu, LIU You-mei, JIANG Dao-hong, LI Guo-qing, HUANG Jun-bin*
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    Sclerotinia stem rot caused by Sclerotinia sclerotiorum is an serious disease on rapeseed worldwide. In this study, infection characteristics of ascospores of S. sclerotiorum on rapeseed leaves and stems were investigated under different temperatures, suspension concentrations, inoculation methods and supplied nutrients conditions with artificial inoculation in greenhouse. Results showed that ascospores germinated and grew rapidly between temperature of 10℃ - 30℃ with the optimum of 20℃ - 25℃. The highest disease incidences and lesion sizes were observed on unwounded or wounded leaves and stems at temperature 20℃ - 25℃. Wounded treatments were more suitable for ascospores infection and leaves were easier to be infected than stems by ascospore suspensions. Both the conditions of 106/mL ascospores and 2% sucrose (or dextrose) were the most favorable for leaf or stem infection. Compared with ascospore suspension inoculum, diseased flowers and mycelial plugs significantly increased the disease incidence.
    Key words: Sclerotinia sclerotiorum; Rapeseed; Ascospore; Infection characteristics

  • CUI Juan, XU Wei, ZHANG Qing-Ling, ZHU Shi-Yu, SHI Shu-Sen*
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     For better prediction with effective integrated management on soybean from Ascotis selenaria in field, temperature effects were studied on A. selenaria development in greenhouse. At a series of temperature gradient (22, 25, 28, 31 and 34 ℃), the worm developmental period, growth rate, survival rate, reproduction, threshold temperature and effective accumulative temperature for different stages on host soybean were investigated. Results showed that development duration of every stage of A. selenaria reduced with increasing temperatures from 22-31℃. There was a positive correlation between temperature and growth rates of egg, larva, pupa and prepupa. Under 34℃ the egg hatching rate decreased sharply and the 6th instar larva stopped developing and died, while 1 to 5 instar larva developed normally. Egg hatching rate was above 98% at 22-28℃,while survival rate of larvae and pupae were the highest at 28℃. Threshold temperature for egg, larva and prepupa were 6.68, 9.10 and 10.97℃, and the effective accumulated temperature were 88.96, 333.41 and 33.97 day-degree respectively. Insect fecundity was the highest at 31℃, with average 754.83 eggs laid per female, followed by 726.28 eggs laid per female at 28℃. 

  • CHENG Chuan-ying, JI Shou-min, ZHANG Feng-wen,YIN Wan-yuan, JIANG Xing-yin*
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    Seed dressing influences of strobilurin fungicides (pyraclostrobin, kresoxim methyl and azoxystrobin) on physiological and biochemical characteristics in peanut seedling were investigated. Growth promotion of peanut seedling was observed after seed dressing with 3 fungicides at dosage of 5, 10, 15, 20 g a.i./10kg seeds. Result showed that 3 fungicides promoted seedling growth significantly at dosage of 10 g a.i./10kg seed. Plant height increased by 19.7%, 15.7% and 15.4% respectively. Root length increased by 26.7%, 26.1% and 8.9%. Ground fresh weight increased by 26.3%, 12.5% and 21.3%. Underground fresh weight increased by 44.4%, 44.4% and 33.3%. Activity of protection enzymes (SOD, POD, CAT) and transaminase (GPT, GOT) were increased. Chlorophyll and soluble protein contents were also increased. Malondialdehyde (MDA) content decreased. Root activity was increased. These 3 fungicides had strengthening effect on peanut seedlings.

  • LIU Jia, ZHANG Yun-hua, MENG Qin-lin, SHI Feng-mei, MA Li-gong, LI Yi-chu, ZUO Yu-hu*
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    To better understand the physiology and biochemistry resistance mechanism of sunflower on Sclerotinia sclerotiorum toxin, changes of β-1,3-glucanase and MDA, soluble sugar, free proline, lignin and cell membrane permeability of sunflower leaves were investigated. 2 sunflower varieties Fengkuiza 1 (Fkz1) and 7101 with different resistance were treated with crude toxin from S. sclerotiorum. The above indexes (except MDA) were significantly changed after toxin treatment especially in resistant cultivar. Cell membrane permeability was significantly damaged by toxin. Resistant Fkz1 had higher ability of damage-resistance than 7101 (susceptible). Results showed that sunflower resistance to S. sclerotiorum had positive correlation with chitinase, β-1,3-glucanase activity, soluble sugar, free proline, lignin content and cell membrane permeability. MDA was negatively correlated with the resistance.

  • ZHANG Liang,LI Pei-wu, ZHANG Qi,ZHANG Wen,YANG Qing-qing,ZHU Jian-guo
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    T-2 toxin was reacted with succinic anhydride to prepare T-2-hemisuccinate (T-2HS). Then,T-2HS was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to obtain the conjugates T-2HS-BSA and T-2HS-OVA. As immunogen, T-2HS-BSA was intradermally injected in Balb/c mice. Then, spleen cells and myeloma cells were fused and selected. Four positive hybridoma cell lines were obtained, which were 1D6, 2A8, 2C5 and 4G3. The antibody from 2C5 has the best sensitivity (expressed by IC50), which was 0.13μg.kg-1. This antibody was against T-2 particularly, and the cross reactivity with HT-2 was 4%. It had no cross reactivity with mycotoxins FB1, DON and ZEN. An indirect competitive ELISA was developed for T-2 toxin detection, and the limit of detection was 0.015μg.kg-1. The detection range (IC20-IC80) of this method was 0.05-57.6μg.kg-1. The recoveries of spiked T-2 toxin, in 0.05-57.6μg.kg-1, were 93.5%-107.5%. The developed method could be used for T-2 toxin detection in many grain and oil products, such as soybean, corn and peanut.

  • ZHU Yan-tao, TIAN Jian-hua
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    To better understand the triquetrum-pod (tri-loculus silique) rapeseed (Brassica napus L.), pod characteristics of 7 selfing populations were investigated. Results showed that the 100% of individual plants had triquetrum-pods among the 7 populations. The average triquetrum-pod per plant (triquetrum-pod rate) was 32.31%-55.78%. About 48.82%-85.71% plants had the triquetrum-pod rate of 30%-70%. The triquetrum-pod rates showed normal distribution among all 7 materials. It suggested that triquetrum-pod trait was a quantitative trait.