Welcome to CHINESE JOURNAL OF OIL CROP SCIENCES,

Archive

  • 2018 Volume 40 Issue 4
    Published: 28 August 2018
      

  • Select all
    |
  • XUChun-fang,XIAOJun-yong,ZHENGMing-ming,CONGHuai-ren*,HUANGFeng-hong
    Download PDF ( )   Knowledge map   Save
    Amethoddetecting10kindsofphenoliccompoundsinhempseedoilsimultaneouslyusingahigh performanceliquidchromatographywasestablished.Thedetectionlimitwasintherangeof0.05to0.12μg/mL withsuperiorcorrelationcoefficientof0.9970to0.9998,respectivelyandtheaveragerecoveryfrom 85% to 108%.Thecontentsoftotalphenolics,totalflavonoidsandinvitroantioxidantactivity(DPPH,FRAP,ABTS+ ·)of8kindsofhempseedoilcomingfromdifferenthabitatsweredetermined.Itwasfoundthatthehempseedoil wasprovidedwith1.20~17.35mgofcoumaricacid/100gtototalphenolicsand7.61~51.72mgofnaringin/100g tototalflavonoid,DPPHscavengingcapacityof9.03~19.71mmolTE/100g,FRAPreductioncapacityof13.12~
    30.09mmolTE/100gandABTS+· scavengingcapacityof29.40~358.37mmolTE/100gwereobtained,respectively.TheresultsturnedoutthatthetotalphenoliccontentsshowedremarkablypositivecorrelationwithDPPH,ABTS+· scavengingcapacityandtheabilityofFRAPreduction,meanwhile,theDPPHscavengingcapacity andFRAPreducingabilitywerepositivelyassociatedwithtotalflavonoidcontents.
  • FENGFei,WANGLei,FUChun-ling,TANMei-lian*,WANGWei,YANXing-chu*,WANGLi-jun
    Download PDF ( )   Knowledge map   Save
    Foreffectivehybridbreedingandpurityidentificationinsunflower,simplesequencerepeat(SSR) polymorphismwasusedtodeterminegeneticdiversityandrelationshipsin124sunflowercultivars,andtheirfingerprintswereconstructedbasedonSSRmarkers.NineteenprimerpairswereselectedandappliedinPCRamplifica
    tionof124cultivars,basedontheirclearandrepeatablepolymorphisms.Itwasshownthatthepolymorphisminformationcontentofthese19primersrangedfrom0.30to0.62,withanaverageof0.41.Clusteringanalysisindicatedthatgeneticdiversityamongthesesunflowercultivarswasrelativelylow,withtheminimumgeneticsimilaritycoefficientof0.62,andtheassociationbetweenpedigrees’geneticdistanceandgeographicaloriginwasnotclear.Usingthe19primerpairs,fingerprintof124sunflowercultivarswasconstructedandallsamplescouldbeclearlyidentifiedexceptSPQ3andSY7.However,therewerenodifferencesinsomelociprobablyduetothenarrowgene flowamongthesesunflowerlines.Theseresultsindicatedthatthegeneticbackgroundofthesetestedcultivarsisrelativelypoor,anditisnecessarytoenrichgeneticdiversitybyexploitingthewildorotherspeciesasbreedingmaterialsinsunflower.

  • LIGuang-hui,WANGXing-jun,SHISu-hua,SUNJin-bo,ZHAOShu-zhen*
    Download PDF ( )   Knowledge map   Save
    Inrecentyears,freshpeanuthasbecomemoreandmorepopularinChinabecauseofitsdelicious taste,richnutrition,andbenefittohumanhealth.Thisreviewmainlysummarizedtheresearchprogressesoffresh peanutinChina,includinggermplasmevaluation,breeding,high-yieldingcultivation,andprocessingmethod. Theexistingconstraintsaffectingfreshpeanutindustry,marketingprospectandtechnicalinnovationprioritieswere alsodiscussed.
  • CAOJin-hua,ZHUJia-cheng,WANGJian-ping,ZHANGShu-fen, WENYan-cheng,HEJun-ping,ZHAOLei,CAIDong-fang,WANGDong-guo
    Download PDF ( )   Knowledge map   Save
    ThecontroleffectsofDiyaling(imidaclopridandphoximusedincombination)appliedinsowing =timeonrapeaphidswereevaluatedduringthewholegrowingseasons.Grainyieldandresidueofthepesticidein rapeseedwereinvestigated.Diyalingwasappliedin3ways(insowingditch,ontopsoilaftercoveredandontopsoil beforecovered)with3dosages.Resultsshowedthatbyapplying900g·hm-2Diyalingontopsoilbeforecovered, morethan90% controlefficiencywasobtainedduringthewholeoccurrenceperiod,and88.6% atthematureperiod.Comparedtocontrol,podnumbersperplant,seedspersilique,1000-grainweightandyieldwereallimprovedsignificantly.Yield-increasingratewas13.7% to25.8%.SpreadingDiyaingonthetopsoilbothbefore andaftercoveringsoil,thegreaterthedosage,thebetterthecontroleffectandthehighertheyield.Controleffectontopsoilbeforecoveredwasbetteratthesamedosage.Rapeseedoilcontentandoleicacidcontentwerebothsignificantlyincreased.Nopesticideresidueinrapeseedwasfound.Thusthemethodwassimpleandefficient,aswell asenvironment-friendly.
  • TIANZhen-qi,WANGSu-ji,BAIBing,WANGXue-qian,LIUJian*,ZHAOKui-jun
    Download PDF ( )   Knowledge map   Save
    Lifeparametersareimportantcharactersforinsectpopulation.Todeterminetheseparametersaccurately,insectsareusuallyrearedindividually.Tostudyeffectsofdifferentrearingmethodsonlifeparameters,novelleaf-discmethod,water-agarmediummethodandfilterpaperrearingmethodwereappliedtorearAphisgly
    cinesMatsumuraat20,23and26℃ inthisstudy.LifetablesofA.glycinesexperimentalpopulationswerebuilt. DifferencesinlifeparametersofA.glycines,suchasnymphstage,adultlongevity,adultfecundity,populationsize,intrinsicrateofincrease,netproductiverate,finiterateofincrease,meangenerationtimeandlifeexpectancy,wereallstudied.Ateachofthesetemperatures,20,23or26℃,therewerenosignificantdifferencesin nymphstageofA.glycinesrearedwiththesethreerearingmethods.WhenA.glycineswererearedwithfilterpaper rearingmethodat20,23or26℃,adultlongevitywere28.68±2.74,20.03±1.89and15.23±1.43dandadult fecunditywere50.39±2.37,43.22±3.45and43.21±3.20No./generation.,Theseongevityandfecundityof adultwerelongerandbiggerthanthatofsoybeanaphidrearedwithnovelleaf-discmethod.TherewerenosignificantdifferencesinpopulationsizesofA.glycinesrearedwithfilterpaperrearingmethodandnovelleaf-discmethodateachtemperature.ItshowedthatthereweretheirowncharactersinthesethreemethodsandthereweresignificantdifferencesinlifeparametersofA.glycinesrearedwiththesemethods.TheseresultswereimportantforselectingrearingmethodinfurtherresearchesonlifecharactersofA.glycines.
     
  • LIYing-hua,WANGKuo,ZHENGYan-hong,LIMin-min,XUChong-jing,YANGXue, WANGChun-sheng,ZHANGYan,LIWen-bin*,ZHAOLin*
    Download PDF ( )   Knowledge map   Save
    PreviousstudiesinourlabshowedthatpolymorphicsiteofSNP-796inthepromotersequenceof GmGBP1genewascloselyrelatedtothegrowthperiodinsoybean.SNP-796Gisanexcellentalleletoshortenthe growthperiodofsoybean.Inthisstudy,theresultsofbioinformaticsanalysisshowedthattheG→Amutationof SNP-796resultedinthevariationofTCT-motiflight-responsiveelement.ThepromoterfragmentofGmGBP1 geneincludingthepolymorphicsiteofSNP-796wasfusedwithreportergeneofLuciferase.ThefunctionofTCTmotifcis- actingelementwasanalyzedbytransientexpressionofAgrobacteriumtumefaciensintobaccoleaves.The resultsshowedthatTCT-motifwasinducedbyshort-dayconditionandcausedthesignificantlyincreasedexpressionofLuciferasegene.TCT-motifcouldincreasethemRNAexpressionofGmGBP1geneundershort-dayconditionstoshortenthegrowthperiodofsoybeanvarieties.
  • HUMing,YAOSheng-li,CHENGXiao-hui,LIUYue-ying,MALi-xin, XIANGYang,HUANGJun-yan,TONGChao-bo,LIUSheng-yi
    Download PDF ( )   Knowledge map   Save
    Formoreinformationofgeneticvariationamongdifferentecotypes,high-depthDNAre-sequencingwasconductedfor3representativecultivarof3ecotypesofBrasicanapus:Oro(springtype),ZS11(semiwintertype) andDarmor-Bzh(wintertype).Amongthem,alargenumberofgeneticvariationsincludingSNP, Indel,structuralvariationandcopynumbervariationweredetected.Bygenomicdistributionofthesevariations, 43983(springvswinter),44592(semi-wintervswinter)and44991(springvssemi-winter)geneswere found.GOenrichmentanalysisshowedthatthegenesweremainlyenrichedinGOcategoriesrelatedtobiological metabolism,developmentalregulationandenvironmentaladaptionetc.Forbetterunderstandingofecotypeformation,genevariationofFLC,VRN,GA,SPL3werefocused.Theywerefoundtoberelatedtofloweringtime,and involvedinplantvernalizationpathway,gibberelinpathway,andagingpathway.Theidentifiedgeneticvariations couldprovidevaluabledataforrevealingthegeneticbasisoftheformationofdifferentecologicaladaptationsofB. napus.

  • NIUNing,LIZhen-xia,JINSu-juan,ZHAOXuan,FUYa-li,JIAOXin,WANGYu-ling,LIZhan-jun*
    Download PDF ( )   Knowledge map   Save
    Tobreedsoybeanvarietieswithhighlightefficiency,photosyntheticcharacteristicsofsoybeangermplasmresourcesinHuanghuaihairegionwerestudiedandthehighphotosyntheticgermplasmswerescreened.The photosyntheticparametersof150soybeangermplasmsatfull-bloomstageweredeterminedbyLI-6400portable photosynthesisapparatus,includingnetphotosyntheticrate(Pn),stomatalconductance(Cs),internalCO2concentration(Ci),transpirationrate(Tr),leafsaturatedvaporpressuredeficit(VPDL),transpirationefficiency (TE)andleafinstantaneouswateruseefficiency(WUE).Resultsshowedthatthereweresignificantdifferencesin photosyntheticgasexchangeparameters.Thecoefficientofvariationofparameterswas5.54% -29.56%,among whichthecoefficientofvariationofTEwasthehighestandthecoefficientofvariationofCiwasthelowest.PnhadsignificantpositivecorrelationswithCsandWUE,andhadanegativecorrelationwithVPDL.Threemaincomponents(stomatalfactor,waterfactorandphotosyntheticfactor)wereselectedbyprincipalcomponentanalysis,and thecontributionrateofvariancewas93.64%.Clusteranalysisof150soybeangermplasm wasdividedinto12 groups,ofwhichthesecondgroupincluded23soybeangermplasmwhichhadhighstomatalconductance,highwateruseefficiencyandhighphotosyntheticefficiency.Therefore,GroupⅡ couldbeusedasasuitablehigh-efficiencysoybeangermplasm.
  • CUICheng,LIShun-da,LIZai-yun*
    Download PDF ( )   Knowledge map   Save
    Asdiploid-likemeioticbehaviorisfundamentalfortheformationandevolutionofnewallopolyploid species,thecytologyandgeneticmechanismsinBrassicaallopolyploidsarereviewed.Although3cultivatedBrassicaallotetraploidsoriginatedfromthepair-wisecrossesbetween3Brassicadiploids,thetrigenomicallohexaploid speciesarenotfoundinnature.Brassicaallohexaploids(2n=54,AABBCC)weresynthesizedusing3crossing strategieswithcombinationsofthegenomesfrom theextantallotetraploidsanddiploids.Theyshowedthediploidizedandnon-diploidizedmeioticbehaviors,andproducedbotheuploidandaneuploidprogenies,suggesting thatgenomestabilityorderwasB>A>C.InthesesyntheticBrassicaallotetraploidsandallohexaploids,cytoplasmicbackgrounds,thecombinationsandrelatednessofthediploidgenomesaffectmeioticpairingandsegregation, andthusthechromosomenumbersandcomplementsinprogenies,resultinginproductionofnovellinesforgenetic researchesandbreeding.
  • FUMing-lian,YUANXiao-yan,ZHANGYun-yun,LIShu-qiong, ZUFeng,TIANZheng-shu,HEBin,XIJun-yu
    Download PDF ( )   Knowledge map   Save
    Promotionofyieldandoiloutputwererestrictedbythefactoroflowerharvestindex.Inthisstudy,200F1DH(doublehaploid)populationandparents,G25(high-harvest-indexinbredline)andH008(lowharvest-indexinbredline)wereplantedinLijangandLincangthatbotharerape-producingareaofearlymaturingandhighyieldinYunnan.Thetraitscloselyrelatedtograinyieldandharvestindex(YHI)werescreened from17traitsincludinglengthofgrowthperiod,yieldperplant,1000grainweight,actualharvestofexperimental plotsandoilcontentofgrain.Theresultsshowedthattherewerehigheryieldperplantandyieldperunitarearesultingfromstrongvegetativeorgan,longfloweringtimeandespeciallymoresiliquesofprimaryandsecondarybranchesinseedlingtransplantationareaofLincang.However,excessivevegetativebodyandexcessivenumberofpod werenotconducivetoincreasingrapeharvestindex.IndirectseedingareaofLijiang,rapeseedlingsarelongand
    shortfloweringperiod,lackofvegetativebody.Thenumberofmaininflorescenceofthehornfruitwasgreaterand thenumberofbranchfruitwasfewerinLijiang.Thefollowingsixtraitsweresignificantlypositivelycorrelatedwithharvestindex,andthesetraitswerepodnumberandkernelsofprimarybranch,kernelnumberofsecondary branch,singleplantpodnumber,yieldperplantandoilcontent.Cultivationofstrongseedlingscouldimprovetheeconomiccharacteristicsofsingleplant,yieldperplant,yieldperunitareaandtheharvestindex.Underthehigh yieldhabitatofYunnan,yieldperplantfavourstheharvestindex.Drymatteryieldatearlyfloweringstagehasanegativeeffectonharvestindex.Thevariationofthepodnumberofprimaryandsecondarybranchesbetweendifferentmaterialsvariedgreatly.ItisaneffectivestrategytoimproverapeharvestindexinhighyieldhabitatinYunnan,andtheseincludedcontrollingdrymatteryieldatearlyfloweringstage,maintainingreasonablepodnumberof primaryandsecondarybranchandthousandgrainweight.
  • GUYue,LIULi-min,CHENFu-lu,FUYong-fu,CHENQing-shan*,ZHANGXiao-mei*
    Download PDF ( )   Knowledge map   Save
    TheproteinsencodedbyFT(GmFTL1-6)genesfromsoybeancanpromoteArabidopsisflowering. However,onlyGmFTL3andGmFTL5havebeenproventobeinvolvedintheregulationoffloweringinsoybean (GlycinemaxL.Merr.).GmFTL3andGmFTL5geneshavehighsequencesimilarity,andisthereanydifference betweentheminfloweringregulationinsoybean?Inthisstudy,promotersofGmFTL3andGmFTL5wereusedto analyzetheexpressiondifferencesbetweenthesetwogenes.pGmFTL3pro::GUSandpGmFTL5pro::GUSvectors wereconstructedandtransformedintoArabidopsisforGUSstaining.TheresultsshowedthatGmFTL3promoter didn’tdriveGUSgeneexpressioninA.thalianaunderlongdayandshortdayconditions,whereasGmFTL5promoterdroveGUSgeneexpression.GmFTL5pro::GUSshowedthespecificityoftissueexpressionatdifferentseedling stages.Infloralorgans,GmFTL5promoterdriveGUSgeneexpressionincalyx,petal,antherandpollengrains. Ontheotherhand,quantitativereal-timePCRresultsshowedthatbothGmFTL3andGmFTL5wereexpressedin soybean,whichwasdifferentfromresultsofGUSstaining.Inconclusion,GmFTL3promoterhavespecies-specific expressioncharacteristics.Itwasonlyactiveinsoybean.TheexpressionofGmFTL5promoterinsoybeanandArabidopsiscouldbedifferent,andGmFTL5genecouldbeassociatedwiththedevelopmentoffloralorgans.
  • LIUBao-lin,ZOUXiao-yun1,SONGLai-qiang,GUANChun-yun*
    Download PDF ( )   Knowledge map   Save
    Theeffectsofcontrolledreleasenitrogen(CRN)fertilizerapplicationonyield,oilcontent,nitrogen absorptionandutilizationofearlymaturerapeseedwerestudied,inordertoprovideascientificbasisforCRNfertilizerapplicationonearlymaturerapeseedindoublecroppingriceareaofsouthernChina.Rapeseedcultivar1358 withshortgrowthperiodwasusedasmaterialin6fieldexperimentsinJiangxiProvinceduring2012-2013.ResultsshowedthatCRNeffectwasconsistentwithnormalnitrogenfertilizationonrapeseed.Yieldincreasedwiththeincreasednitrogenapplicationrate.TheyieldinthesameamountofCRNwashigherthanthatinthesamenitrogen application.CRNapplicationimprovedthenitrogenfertilizerutilizationrateatthesametime.Inthesameamount nitrogenapplicationrateof180kg/hm2,therapeseedyield,oilproductionandthenitrogenuseefficiencyinCRN treatmentwithone-timeapplicationwerehigherthanthoseinnormalnitrogenfertilizationtreatmentwiththreetimeapplication(basefertilizerapplied60%,seedlingfertilizerapplied20% andboltingfertilizerapplied20%) by7.64%,8.30% and2.27percentagepointrespectively.Theoptimum CRNfertilizerapplicationratewas
    150kg/hm2underdelayedsowingconditioninwinterfallowpaddyofJiangxiProvince.
  • JIANGHuan-huan,WANGTong,YUShan-lin,CEHNMing-na, WANGMian,CHENNa1,PANLi-juan,QIPei-shi*,CHIXiao-yuan*
    Download PDF ( )   Knowledge map   Save
    The14-3-3sareagroupofproteinsthatareubiquitouslyfoundineukaryotes.Plant14-3-3 proteinsareencodedbyalargemultigenefamilyandinvolvedinsignalingpathwaystoregulateplantdevelopmentandprotectionfromstress.Inthisstudy,fourteenAd14-3-3genesincluding8ε-typeand6non-εtypewereidentifiedfromArachisduranensisgenomeandthirteenAi14-3-3genesincluding6ε-typeand7non-εtype
    fromArachisipaensisbyusingHMM(hiddenMarkovmodel)scanstrategy.Thephysicalandchemicalproperties, genelocation,genestructureandupstreamregulationsequencewerefurtheranalysed.Resultsshowedthatno14-3-3genewasonchromosome1and6,boththreememberswereonchromosome4and7.The14-3-3genes familieswerehighlyconservationwith6exonsinε-typegenestructureand3-4exonsinnon-εtype.Multiple cis-elementsresponsivetodifferenthomonesandenvironmentalstresseswerefoundinupstreamsequencesofall 14-3-3genes,indicatingthattheyparticipatedinphysiologicalandadversitystressresistanceinpeanut.
  • FUXiao-ji,TANGAi-qing,MINHua,OYANGLing-hua,XIONGHui-wei, ZHUXue-jing,FENGJian-xiong,XINGSheng-ping*
    Download PDF ( )   Knowledge map   Save
    Moisturephasestateandtransportwerestudiedbynuclearmagneticresonance(NMR)andnuclear magneticresonanceimaging(MRI)toinvestigatethechangesoftransverserelaxationtime(T2)andMRIimages duringtheprocessofpeanutssoaking.Theresultsshowedthatinternalmoistureofseedcouldbedividedtobound waterandfreewaterduringsoakingprocessaccordingtodifferenttransverserelaxationtimeT2.Itwasfoundthattotalmoisturecontentwaskeptincreasinginsoakingprocess,contentoffreewaterincreasedwhereasboundwater maintainedrelativlystableaccordingtothedifferenceofsignalamplitudeinT2inversionspectrum.MRIimageof peanutseedsduringsoakingcouldmanifesttheinternalwaterdynamicflowprocesswithinseeds.Mapanalysis showedthatwaterfirstpenetratedthemembraneofseedtip(nearembryos),andthenthickcotyledonsintoseed, andfinallyreachedtheinteriorpart,buttheimmersionwasveryslow.NMRandMRIcouldprovidedirectreferenceforitsmoisturechangesandresultsofthisstudycouldprovidedirectreferencesandtechnicalsupportforpeanutssoaking.
  • XINGXing-hua,FANGChuan-wen,QIYu-jun,XUZe-jun, WANGXiao-jun,SUNDong-lei,BIANNeng-fei,WANGXing*
    Download PDF ( )   Knowledge map   Save
    Inordertoinvestigatetheregulatoryroleofsodiumdiethyldithiocarbamate(DDTC)inrootnitrogenmetabolismofwaterlogging-stressedsoybean,twosoybean(Glycinemax)varietiesXudou18(waterlogging-sensitive)andNannong1138-2(waterlogging-tolerant)wereusedtostudytheeffectofDDTConrootactivity,concentrationsofnitrogenouscompoundsandactivitiesofnitrogenmetabolismenzymeofsoybeanrootsundershorttimewaterloggingstress(waterleveluptocotyledonnodeofsoybean)atearlyfloweringstage(R1)withpotexperiment.OnedayaftersprayingDDTCordistilledwateratR1stage,waterloggingstresswasperformedfor8d.The resultsshowedthatunderwaterloggingstress,comparingtoXudou18,Nannong1138-2hadlessdecreaseinrootactivity,nitrogenfixingabilityandtranslocationabilityofnitrogenouscompoundsofroots,lessincreaseinendopeptidase(EP)activityandNH4 + -Ncontentofroots,whileagreaterincreaseintheactivitiesofnitratereductase (NR)andglutaminesynthethase(GS)andthecontentofNO3 - -Nandfreeaminoacids(FAA)ofroots.DDTC exogenousDDTCcouldregulatenitrogenmetabolismforsoybeanrootsandnodules,whichwasbeneficialtoimprovetheresistancetowaerloggingstressforsoybeanplants.
  • FANYong-hai§,CHANGWei§,ZHAOBin-yan,WEISi-yu,DUANHui-chun,LIJia-na,LUKun*
    Download PDF ( )   Knowledge map   Save
    OrthogonaltestandquadraticorthogonalregressionexperimentwereusedtoanalyzetheeffectofenvironmentalfactorsonseedlinggraftingofBrassicanapus.Theconditionincludedmoisture,shading,daytime, cotyledonremovalornot,temperature,relativeairhumidity,withZhongshuang11asthegraftingmaterial(stock
    andscion).Threedifferentstagesofrapeseedseedlingsweregraftedbyflat,splice,holeinsertion,side,approachmethod,respectively.Thebestgraftingmethodforeachstagewascomprehensivelyevaluated.Resultsindicated thatamongthoseenvironmentalfactors,themoisturehadthegreatestsignificanteffectongraftingsuccessrate,followedbyshading.However,daytimeandcotyledonkeptornothadlittleeffectonsuccessrate.Quadraticorthogonalregressionanalysisoftemperatureandhumidityshowedthattheoptimaltemperatureforhealingwoundwas 24.4℃ andrelativeairhumiditywasabout88%.Graftedseedlingswereeasytodevelopadventitiousrootsonthe woundhealingofscionunderhighairhumidity,andreducingairhumidityappropriatelycouldinhibitadventitious root.Thesuccessratesweremorethan85% amongthe5graftingmethodswhentheseedlingsweregraftedat10d.
    For20-dayseedlings,thesuccessratereached93.3% usingtheapproachgrafting,whiletheflatandsplicewas 56.7% and46.7%,respectively.Thesuccessratesof40-dayseedlingsweresignificantlydecreasedunderthe 5graftingmethods.Successratesofapproach,holeinsertionandsidemethodwere45%,35%and23.3%,re spectively,whiletheflatandsplicewereonly13.3% and10% respectively.
  • CHENJu-hong,CUIJuan,TANGJia-wei,BIRui,ZHANGJin-ping,SHIShu-sen*
    Download PDF ( )   Knowledge map   Save
    DevelopmentandfecundityofRiptortuspedestrisFabriciusweretestedatfivedifferenttemperature (16,20,24,28,and32℃).Theresultsshowedthatdevelopmentalduration,developmentalrate,andsurvivalrateofR.pedestrisweresignificantdifferenceattestedfivetemperatures.LifecycleofR.pedestriscouldnotcom
    pleteat16℃,noadultsovipositionat32℃.ThedevelopmentaldurationofeachstageofR.pedestrisdecreased withtemperaturesincreasingfrom16to32℃,andasignificantpositivecorrelationbetweendevelopmentalrateand temperaturewasconfirmedbyfittingequation.Thedevelopmentalthresholdtemperaturesoftheegg,nymphandadultwere12.58,15.98,and15.27℃,respectively,andcorrespondingeffectiveaccumulativetemperatureswere 102.32,203.28,and103.94degree-days.thehighestovipositionofsinglefemalewas87.39eggsat24℃,itwas2.78timeshigherthan20℃ and5.34timesthan28℃.ThehighestpopulationtrendindexofR.pedestriswas I=14.30at24℃,itwas2.34timeshigherthan20℃,and4.27timeshigherthan28℃,respectively.TemperaturealsohadasignificanteffectonthesurvivalrateofR.pedestris.Theresultscouldprovideascientificbasisfor thepredictionoftheoccurrenceperiodandintegratedmanagementofR.pedestris.
  • ZHUOMe-cao,GUOWen-wen,YANGGuang-huan,DUNXiao-ling,LIMeng-han*,DANBa*
    Download PDF ( )   Knowledge map   Save
    InordertostudythegeneticdiversityofTibetyellowrapeseedgermplasmresourcesandimproveits breedingefficiency.Thegeneticdiversityof30BrassicarapaL.yellowseedrapeseedwasanalyzedbyScoTmethod,andanalyzedcomprehensivelyincombinationwithbiologicalcharacteristicsinformation.Throughscreeningof50
    SCoT-labeledprimers,24primerswithgoodrepeatability,highpolymorphism,andabundantgeneticinformation wereobtained.Atotalof214DNAsiteswereobtainedbyPCRamplificationofthereferencegermplasm,ofwhich 182werepolymorphicandtheaveragepercentageofpolymorphiclociwas87.33%.UsingNTSYSPc2.10esoftwareanalysis,thesimilaritycoefficientofthetestedgermplasmresourceswerebetween0.473and0.930.Theunweighted groupaveragemethod(UPGMA)clusteranalysiswasusedtogenerateadendrogram,whichcouldbeutilizedtotest 30Tibetancabbagetypes.Rapeseedvarietieswerecompletelyseparated,andthesimilaritycoefficientwas0.797, whichcouldbedividedinto5groups.ThegeneticdiversityofTibetyellowrapeseedwasrelativelyabundant.
  • SONGXiao-yu,MAOTing-ting,WANGLi-wei,LIULi-feng,LIXiao-na,SUNShi,HANTian-fu*
    Download PDF ( )   Knowledge map   Save

    Floweringtimeisanimportanttraitdeterminingsoybeanecologicaladaptabilityandyield.Itisofgreatsignificancetounderstandthegeneticbasisandthemolecularmechanismoffloweringregulationinsoybean.Inthecurrentstudy,87soybeanvarietiesfrom3majorsoybeanproducingareas(NorthernSpring-sowingRegion,
    Huang-Huai-HaiRiversValleyandSouthChina)ofChinaand31fromUSAwereplantedinspringandsummerrespectivelyinBeijing.Dataforfloweringtimeunder10sowingdateswereobtained.SNPanalysiswascarriedoutusingIlluminaBARCSoySNP6KiSelectBeadChip.Throughtheanalysisofpopulationstructure,Neighbor-joining
    andPCA,soybeanvarietieswereclassifiedinto2sub-groups.Thegenome-wideassociationstudy(GWAS)betweenthefloweringtimeand5126SNPswereimplementedwithPCA+KmodelusingTASSELsoftware.Atotalof18significantlociassociatedwithsoybeanfloweringtimewereidentified.Amongtheloci,14weredetectedinmorethan2environments,indicatingthattheselociwerelessinfluencedbytheenvironmentalfactorsandcouldstablyexpressacrossdifferentenvironments;4weredetectedonlyinasingleenvironment,suggestingthattheyweresusceptibletophoto-thermalconditions.Newcandidategenesmightexistintheunreortedloci,whichwerelocated at3,4,5,9,10,14,16and17chromosomesunderlyingfloweringtime.31candidategeneswerepredicatedthroughannotation,inwhich2geneshavebeenprovedtobeinvolvedinfloweringregulationofsoybean,andGlyma03g33470,Glyma11g15650,Glyma05g01510andGlyma19g07162weresoybeanorthologsoftherelatedflowergenesinArabidopsis,indicatingthatthese4geneswereunderlyingfloweringtimeinsoybean.

  • FANHui-ling,BAISheng-wen*,LIUZhao,LIZhen-zhou,HEZhi-jun,ZHUXue-feng,QINMing-gang
    Download PDF ( )   Knowledge map   Save
    ToassessthetoleranceofE.sativatomixedsalt-alkalineindifferentgrowthphase,andtoreveal physiologicalmechanismsrespondingtosalt-alkalinestress,theexperimentswereconductedusingdifferentconcentrationofsalt-alkalineduringgermination,seedlingstageandadultstage.Ourresultsshowedthat:(1)At germinationstage,initialgerminationwasdelayedbuttheseedvigorwasnotlostwhenstressedunderlowconcentrationof20mmol·L-1and30mmol·L-1mixedsalt-alkaline.Under40mmol·L-1salt-alkalinestress,the germinationpotentialityandgerminationrateweresignificantlylowerthanthatofthecontrolgroup.Whenthecon
    centrationwashigherthan50mmol·L-1,theseedvigorwerelostcompletely.(2)Relativewatercontentofleaves (RWC)hadnegativecorrelationwiththelevelofsalt-alkalineconcentrationandthestresstime.Whenthesaltalkalineconcentrationwashigherthan40mmol ·L-1,RWCwasdecreasedsignificantly,butmalondialdehyde
    (MDA)contentwasincreasedobviously.Bothprolineandsolublesugarcontentweresignificantlyincreasedwith theincreasingofsalt-alkalineconcentrationandtimeprolong,butthesolubleproteincontentwasreducedgradually,whichindicatedthattheadaptationofE.sativaseedlingtosaline-alkalistressdependedpartlyontheaccumulationoforganicmaterialsuchassolublesugarandprolineetc.(3)Atadultstage,effectsofsalt-alkaline stressonplantheightandfloweringperiodwereconsidered,therewerenosignificantlydifferencebetweentheex perimentalgroupandcontrolgroupwhenconcentrationofsalt-alkalinewaslow(20and30mmol·L-1).When salt-alkalineconcentrationwas40mmol·L-1to50mmol·L-1,comparedwithCK,theexperimentalgrouppresentedshorterplantsheight,delayedfloweringperiodandlessnumberofefficienthornsperplant.E.sativafailedto seedwhentheconcentrationofsalt-alkalinewas60mmol·L-1.Vegetativeandreproductivegrowthwereinhibitedseriously,orevendeathastheconcentrationofsalt-alkalinewas70mmol·L-1.Rootlength,freshweight, dryweightandwatercontentwereinhibitedundersalt-alkalinestressofdifferentconcentrations,especiallythe freshrootweight.Whentheconcentrationwaslessthan30mmol·L-1,rootlengthwaslongerthanCK,whileit wassignificantlyshorterthanCKwhentheconcentrationwashigherthan40mmol·L-1.Inconclusion,salt-al kaline(NaCl+Na2SO4+NaHCO3+Na2CO3)toleranceinE.sativaatgerminationstageandadultstageweredifferent,solublesugarandprolineasthemainorganicsubstancesplayedanimportantroleinadaptingtohighsaltalkalinestress.
  • WANGLei,WANGHui-zhong,OuRan,LIWen-bin,LINChun*,WANGShao-dong*
    Download PDF ( )   Knowledge map   Save
    Soybeanseedstorageproteinisgraduallysynthesizedandaccumulatedduringseedformation,and graduallydegradesduringseedgermination.Differentsoybeansorageproteinscanaltertheirnutritionalandfunctionalqualitiesbyaffectingtheproteinproperties.Lipoxygenaseisthesourceofthesmellofbeans.7Sglobulinand 11Sglobulin,includinglipoxygenaseisthemaincomponentofsoybeanstorageroteins,andithasbecomeanimportantgoalfortheimprovementofsoybeanquality.Thisarticlemainlyintroducesthelatestresearchprogressofgeneticsoybeanproteinimprovementforlipoxygenasedeficiency,11Sglobulindeficiencycoupledwithincreased7S globulincontent,7Sglobulindeficiencycoupledwithincreased11Sglobulincontent,and7Sglobulin-lipoxygenasedoubledeficiency.ItisimportanttoprovidereferenceforChinesesoybeanqualityimprovementbreeding.