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中国油料作物学报 ›› 2019, Vol. 41 ›› Issue (5): 788-.doi: 10.19802/j.issn.1007-9084.2019080

• 土肥植保 • 上一篇    下一篇

盐碱地大豆根内促生拮抗细菌DCX的分离及鉴定

荆瑞勇(1978-),男,博士后,副教授,研究方向:微生物资源开发与利用。E-mail:jry_2002@126.com   

  1. 1.黑龙江八一农垦大学/黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室, 黑龙江大庆,163319
    2.黑龙江八一农垦大学农学院,黑龙江大庆,163319;3.黑龙江省农业科学院植物苗木研究所,黑龙江哈尔滨,150086
  • 出版日期:2019-10-28 发布日期:2019-12-20
  • 通讯作者: 郭永霞(1970-),女,博导,教授,研究方向:农业生物技术。E-mail:gyxia@163.com
  • 基金资助:
    国家重大研发计划(2018YFD0300104,2018YFD0200200);农垦总局“十三五”重点科技计划项目(HN tK135-02-02);黑龙江省博士后资助项目(LBH-Z15189);大庆市科技局指导项目(zd-2016-109);博士启动基金(XDB-2015-19);黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室开放基金(201710);黑龙江八一农垦大学三纵三横项目(TDJH201809)

Isolation and identification of an endophytic, antagonistic and growth promoting bacteria DCX from soy? bean roots in saline-alkali soil

  1. 1. Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Re? gion, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 
    2. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. Research Institute of Vi? rus-free Seedling, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Online:2019-10-28 Published:2019-12-20

摘要:

根系内生细菌资源的开发与利用是绿色农业发展的研究重点,而盐碱地大豆根内生细菌的分离与鉴定鲜有报道。本文以大庆盐碱地大豆根系为材料,分离纯化大豆内生细菌,采用平板对峙法和比色法测定内生细菌的抑菌能力和分泌IAA能力,选取抑菌谱广且分泌IAA能力强的株菌,通过检测形态学特征、生理生化性质、结构基因和功能基因序列的系统发育分析,明确细菌的分类地位。结果表明从大庆盐碱地大豆根内分离获得的一株内生细菌DCX,对10种植物病原真菌均具有拮抗作用,其中,对玉米新月弯孢菌、大豆菌核病菌、稻瘟病菌、禾谷镰刀菌的抑菌率分别为50.00%、78.12%、56.25%、43.75%,分泌IAA的能力为2.56±0.41μg·mL-1(n=6)。经形态学、生理生化试验及系统进化分析鉴定,DCX菌株属于解淀粉芽孢杆菌(Bacillus amyloliquefaciens),该菌株在pH11、NaCl浓度 10%的NA培养基中生长良好,可为盐碱地的作物病害防治提供资源。

关键词: 大豆根内生细菌, 鉴定, 解淀粉芽孢杆菌

Abstract:

Development and utilization of endophytic bacteria have been the focus of green agriculture, isola?tion and identification of endogenous bacteria in soybean root in saline-alkali soil has rarely been reported. In this study, soybean endogenous bacteria were isolated and purified from the soybean root in Daqing saline alkali field by root surface sterilization and grinding. Plate confrontation and colorimetric method were used to measure the ability of bacteriostasis and secreting IAA for selecting strains with wide antimicrobial spectrum and secreting more IAA. Morphological identification, physiological and biochemical analysis, phylogenetic analysis of structural genes and functional genes were used to identify the taxonomic position of isolated bacteria. The results showed that a DCX strain was isolated from soybean roots in Daqing saline alkali field. The strain DCX had the antimicrobial ability to 10 plant pathogenic fungi. The bacteriostasis rates of strain DCX for Curvularia crescent, Sclerotinia Sclerotinia (Lib.) de Bary, Magnaporthe grisea, and Fusarium graminearum Schw were 50.00%, 78.12%, 56.25% and 43.75% respectively, their ability to secrete IAA was 2.56 ± 0.41 μg·mL-1 (n=6). DCX strain was identified as Bacillus amy? loliquefaciens, and the strain grew well in NA medium under pH11 and salt concentration 10% condition. Their re? sults provided resource for prevention and controlling crop disease in saline-alkali soil.