Aux/IAA基因家族鉴定及对大豆茎尖发育的调控作用分析

doi:10.19802/j.issn.1007-9084.2020070

摘要/Abstract

摘要： Aux/IAA 基因家族在植物茎尖发育过程发挥重要作用。为了探究大豆Aux/IAA 基因家族在大豆茎尖发育过程的调控作用，本文以拟南芥Aux/IAA 基因家族蛋白序列为参照鉴定了大豆全基因组Aux/IAA 家族基因，包括63个成员；然后以拟南芥、鹰嘴豆和大豆的Aux/IAA 家族为研究对象，比对这些基因全长氨基酸序列并构建进化树，结果表明三种作物的Aux/IAA 家族成员间亲缘关系差异明显，进化过程中同源重组频率不同；进而利用RNA-seq技术分析东农594（DN）、Charleston（CH）及二者杂交后代的RIL群体的高矮秆池（WH、WS）和F2群体的高矮秆池（JH、JS）的差异表达基因及其功能，共有17个Aux/IAA 基因在3组材料中差异表达。CHvsDN组有15个差异表达基因，JHvsJS组有2个，WHvsWS组只有1个；其中，Glyma.10G180100 在JHvsJS组和WHvsWS组均差异表达，这些结果为进一步研究大豆Aux/IAA 基因家族的功能及调控茎尖发育提供理论依据。

关键词: 大豆, Aux/IAA 基因家族, 茎尖, 发育调控

Abstract:

The Aux IAA gene family plays an important role in the development of plant shoots. In order to explore the regulatory role of Aux/IAA gene family in the development process of soybean shoots, the protein sequences of Aux/IAA gene family of Arabidopsis thaliana were used in current study as a reference to identify the Aux/IAA family genes in soybean genome. There were 63 Aux/IAA gene members in soybean genome. Then the full-length amino acid sequences of Aux/IAA family genes identified from Arabidopsis thaliana and chickpea and soybean were compared to construct an evolutionary tree. The results showed that the genetic relationship between Aux/IAA family members was significantly different, and the frequency of homologous recombination during the process of plant evolution was different. The genes were identified which expressed differentially between 594 (DN) and Charleston (CH) and between the high pool (WH) and short pool(WS) of their RIL population and the high pool (JH) and short pool (JS) of their F2 population. A total of 17 Aux/IAA genes are differential expression. There were 15 genes expressed differentially in the CHvsDN group, 2 in the JHvsJS group, and only 1 in the WHvsWS group. Furthermore,Glyma.10G180100 was differentially expressed in the JHvsJS group and the WHvsWS group. These results give a deep understanding about the Aux/IAA gene family in soybean and provide a theoretical basis for regulating the development of soybean shoots.

Key words: soybean, Aux/IAA gene family, shoot tips, development and regulation

中图分类号:

中图分类号：S565.1

刘婷婷, 李艳燕, 宁晓霜, 刘志华, 姜振峰, 李文滨. Aux/IAA基因家族鉴定及对大豆茎尖发育的调控作用分析[J]. 中国油料作物学报, 2021, 43(4): 648-.

LIU Ting-ting, LI Yan-yan, NING Xiao-shuang, LIU Zhi-hua, JIANG Zhen-feng, LI Wen-bin. Identification of Aux/IAA gene family and the regulation to apical bud development in soybean[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(4): 648-.

参考文献

［1］ Larsson E，Vivian-Smith A，Offringa R，et al. Auxin homeostasis in Arabidopsis ovules is anther-dependent at maturation and changes dynamically upon fertilization［J］. Front Plant Sci，2017，8：1735. DOI：10.3389/fpls.2017.01735.

［2］ Li Z X，Zhang L F，Li W F，et al. MIR166a affects the germination of somatic embryos in larixleptolepis by modulating IAA biosynthesis and signaling genes［J］. J Plant Growth Regul，2017，36（4）：889-896. DOI：10.1007/s00344-017-9693-7.

［3］ Park S H，Elhiti M，Wang H，et al. Adventitious root formation of in vitro，peach shoots is regulated by Auxin and ethylene［J］. Scientia Horticulturae，2017，226：250-260. doi：10.1016/j.scienta.2017.08.053

［4］ Tiwari S B，Wang X J，Hagen G，et al. AUX/IAA proteins are active repressors，and their stability and activity are modulated by auxin［J］. Plant Cell，2001，13（12）：2809-2822. DOI：10.1105/tpc.010289.

［5］ Dharmasiri N，Dharmasiri S，Estelle M. The F-box protein TIR1 is an Auxin receptor［J］. Nature，2005，435（7041）：441–445. doi：10.1038/nature03542

［6］ Gray W M，Kepinski S，Rouse D，et al. Auxin regulates SCF（TIR1）-dependent degradation of Aux/IAA proteins［J］. Nature，2001，414（6861）：271-276. doi：N/A

［7］ Maraschin Fdos S，Memelink J，Offringa R. Auxin-induced， SCF （TIR1） -mediated poly-ubiquitination marks Aux/IAA proteins for degradation［J］. Plant J.，2009，59：100-109. DOI：10.1111/j.1365-313X.2009.03854.x

［8］ Reed J W. Roles and activities of Aux/IAA proteins in Arabidopsis［J］. Trends Plant Sci，2001，6（9）：420-425. DOI：10.1016/s1360-1385（01）02042-8.

［9］ Tiwari S B，Hagen G，Guilfoyle T J. Aux/IAA proteins contain a potent transcriptional repression domain［J］.Plant Cell，2004，16（2）：533-543. DOI：10.1105/tpc.017384.

［10］ Hagen G，Guilfoyle T. Auxin-responsive gene expression：genes，promoters and regulatory factors［J］. Plant Mol Biol，2002，49（3/4）：373-385. DOI：10.1023/a：1015207114117.

［11］ Abel S，Oeller P W，Theologis A. Early auxin-induced genes encode short-lived nuclear proteins［J］. PNAS，1994，91（1）：326-330. DOI：10.1073/pnas.91.1.326.

［12］ Kim J，Harter K，Theologis A. Protein-protein interactions among the Aux/IAA proteins［J］. PNAS，1997，94（22）：11786-11791. DOI：10.1073/pnas.94.22.11786.

［13］ Audran-Delalande C，Bassa C，Mila I，et al. Genomewide identification，functional analysis and expression profiling of the Aux/IAA gene family in tomato［J］. Plant Cell Physiol，2012，53（4）：659-672. DOI：10.1093/pcp/pcs022.

［14］ Wang Y，Deng D，Bian Y，et al. Genome-wide analysis of primary auxin-responsive Aux/IAA gene family in maize（Zea mays L.）［J］. Mol Biol Rep，2010，37（8）：3991-4001. DOI：10.1007/s11033-010-0058-6.

［15］ Jain M，Kaur N，Garg R，et al. Structure and expression analysis of early auxin-responsive Aux/IAA gene family in rice（Oryza sativa）［J］. Funct Integr Genomics，2006，6（1）：47-59. DOI：10.1007/s10142-005-0005-0.

［16］ Shen C，Yue R，Yang Y，et al. Genome-wide identification and expression profiling analysis of the Aux/IAA gene family in Medicago truncatula during the early phase of Sinorhizobium meliloti infection［J］. PLoS One，2014，9（9）：e107495. DOI：10.1371/journal.pone.0107495.

［17］ Kalluri U C，Difazio S P，Brunner A M，et al. Genomewide analysis of Aux/IAA and ARF gene families in Populus trichocarpa［J］. BMC Plant Biol，2007，7：59. DOI：10.1186/1471-2229-7-59.

［18］ Ziauddin A，Kasha K J. Long-term callus cultures of diploid Barley（Hordeum vulgare）. I. Auxin effects on culture initiation and maintenance ［J］. Euphytica，1990，48（2）：171-176. DOI：10.1007/bf00037197.

［19］ He C Y，Cui K，Zhang J G，et al. Next-generation sequencing-based mRNA and microRNA expression profiling analysis revealed pathways involved in the rapid growth of developing culms in Moso bamboo［J］. BMC Plant Biol，2013，13：119. DOI：10.1186/1471-2229-13-119.

［20］ Ross J J，O’Neill D P，Rathbone D A. Auxin-gibberellin interactions in pea：integrating the old with the new［J］. J Plant Growth Regul，2003，22（1）：99-108. DOI：10.1007/s00344-003-0021-z.

［21］ Ross J J，O'Neill D P，Wolbang C M，et al. Auxin-gibberellin interactions and their role in plant growth［J］. J Plant Growth Regul，2001，20（4）：336-353. DOI：10.1007/s003440010034.

［22］ Kende H，van der Knaap E，Cho H T. Deepwater rice：a model plant to study stem elongation［J］. Plant Physiol，1998， 118 （4）： 1105-1110. DOI： 10.1104/pp.118.4.1105.

［23］ Bates G W，Cleland R E. Protein synthesis and auxin-induced growth：Inhibitor studies［J］. Planta，1979，145（5）：437-442. DOI：10.1007/BF00380097.10.2307

［24］ Singla B，Chugh A，Khurana J P，et al. An early auxinresponsive Aux/IAA gene from wheat （Triticum aestivum）is induced by epibrassinolide and differentially regulated by light and calcium［J］. J Exp Bot，2006，57（15）：4059-4070. DOI：10.1093/jxb/erl182.

［25］王一，杨文钰，张霞，等. 不同生育时期遮阴对大豆形态性状和产量的影响［J］. 作物学报，2013，39（10）：1871-1879. DOI：10.3724/SP.J.1006.2013.01871.

［26］ Kloosterman B，Visser R G，Bachem C W. Isolation and characterization of a novel potato Auxin /indole-3-acetic acid family member（StIAA2）that is involved in petiole hyponasty and shoot morphogenesis［J］. Plant Physiol，2006，44（11-12）：766-775. doi：10.1016/j.plaphy.2006.10.026

［27］ Fukaki H，Nakao Y，Okushima Y，et al. Tissue-specific expression of stabilized SOLITARY-ROOT/IAA14 alters lateral root development in Arabidopsis［J］. Plant J，2005， 44 （3）： 382-395. DOI：10.1111/j. 1365-313x.2005.02537.x.

［28］ Liscum E，Reed J W. Genetics of Aux/IAA and ARF action in plant growth and development［J］. Plant Mol Biol， 2002， 49（3/4）： 387-400. DOI：10.1023/A：1015255030047.

［29］ Wang H，Bouzayen M. The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis［J］. Plant Cell，2005，17（10）：2676–2692. doi：10.1105/tpc.105.033415

［30］ Gao J，Cao X，Shi S，et al. Genome-wide survey of Aux/IAA gene family members in potato（Solanum tuberosum）：Identification，expression analysis，and evaluation of their roles in tuber development［J］. Biochem Biophys Res Commun，2016，471（2）：320-327. DOI：10.1016/j.bbrc.2016.02.013.

［31］ Wang S K，Bai Y H，Shen C J，et al. Auxin-related gene families in abiotic stress response in Sorghum bicolor［J］. Funct Integr Genom，2010，10（4）：533-546.DOI：10.1007/s10142-010-0174-3.

［32］ Jung H，Lee D，Choi Y D，et al. OsIAA6，a member of the rice Aux/IAA gene family，is involved in drought tolerance and tiller outgrowth［J］. Plant Sci，2015，236：304–312. doi：10.1016/j.plantsci.2015.04.018654

［33］ Hu W，Zuo J，Hou X，et al. The auxin response factor gene family in banana：genome-wide identification and expression analyses during development，ripening，and abiotic stress［J］. Front Plant Sci，2015，6：742. DOI：10.3389/fpls.2015.00742.

［34］孙雪丽，刘范，田娜，等. 香蕉Aux/IAA基因家族的全基因组鉴定及表达分析［J］. 园艺学报，2019，46（10）： 1919-1935. DOI： 10.16420/j. issn. 0513-353x.2018-0743.

［35］ Sato A，Yamamoto K T. Overexpression of the non-canonical Aux/IAA genes causes auxin-related aberrant phenotypes in Arabidopsis［J］. Physiol Plant，2008，133（2）：397-405. DOI：10.1111/j.1399-3054.2008.01055.x.

［36］ Dreher K A，Brown J，Saw R E，et al. The Arabidopsis Aux/IAA protein family has diversified in degradation and auxin responsiveness［J］. Plant Cell，2006，18（3）：699-714. DOI：10.1105/tpc.105.039172.

［37］ Middleton A M，King J R，Bennett M J，et al. Mathematical modelling of the Aux/IAA negative feedback loop［J］. Bull Math Biol，2010，72（6）：1383-1407. DOI：10.1007/s11538-009-9497-4.

［38］ Reed J W. Roles and activities of Aux/IAA proteins in Arabidopsis［J］. Trends Plant Sci，2001，6（9）：420-425. DOI：10.1016/s1360-1385（01）02042-8.

［39］ Singh V K，Jain M. Genome-wide survey and comprehensive expression profiling of Aux/IAA gene family in chickpea and soybean［J］. Front Plant Sci，2015，6：918. DOI：10.3389/fpls.2015.00918.

[1]	杨永庆, 陈圣男, 李欣欣, 赵青松, 付亚书, 杨春燕, 张孟臣, 廖红. 接种根瘤菌环境下大豆叶形遗传及QTL定位分析[J]. 中国油料作物学报, 2021, 43(5): 825-.
[2]	徐影, 于镇华, 李彦生, 金剑, 王光华, 刘晓冰, . 大气CO₂浓度升高对大豆不同器官碳水化合物积累的影响[J]. 中国油料作物学报, 2021, 43(5): 859-.
[3]	王华美, 任春元, 金喜军, 王孟雪, 曹亮, 张明聪, 赵强, 于高波, 张玉先, . 外源褪黑素对高氮下大豆氮代谢及生长发育的影响[J]. 中国油料作物学报, 2021, 43(5): 872-.
[4]	苌兴超, 王雪松, 张沿政, 景雅, 陈龙, 赵佳梁, 房庆伟, 宋春晓, 李永光, 李文滨. 大豆GmPUB32 基因的克隆及耐盐功能分析[J]. 中国油料作物学报, 2021, 43(4): 638-.
[5]	王大刚, 陈圣男, 于国宜, 李杰坤, 韩千笑, 吴倩, 胡国玉, 黄志平. 1983-2019年安徽省夏大豆品种主要性状变化趋势分析[J]. 中国油料作物学报, 2021, 43(3): 510-.
[6]	代玥, 闫伟棋, 姜雪, 杨新宇. 多重PCR技术检测4种大豆镰孢菌根腐病病原[J]. 中国油料作物学报, 2021, 43(2): 307-.
[7]	常小丽, 王对平, Muhammad Naeem, 李红菊, 武晓玲, 雍太文, 杨文钰. 四川带状套作大豆种腐病菌的分离与鉴定[J]. 中国油料作物学报, 2021, 43(2): 314-.
[8]	赵阳, 金龙国, 王步军. 706份常规大豆品系转基因成分筛查及检测[J]. 中国油料作物学报, 2021, 43(1): 117-.
[9]	李允静, 万丹凤, 刘标, 肖芳, 李晓飞, 武玉花, 李俊, 高鸿飞, 沈文静, 李均, 朱莉, 吴刚. 转基因大豆CP4-EPSPS蛋白的降解规律研究[J]. 中国油料作物学报, 2021, 43(1): 124-.
[10]	王成, 姚俊津, 高越, 王雅偲, 解美霞, 赵新, 兰青阔, 王永. 转G2-EPSPS 和GAT 基因的耐除草剂大豆ZH10-6对田间生物多样性的影响[J]. 中国油料作物学报, 2021, 43(1): 141-.
[11]	徐晓丽, 纪艺, 陈笑芸, 王鹏飞, 缪青梅, 来勇敏, 徐俊锋. 转基因大豆中外源基因g10evo-epsps 的qPCR检测方法的建立和验证[J]. 中国油料作物学报, 2021, 43(1): 110-.
[12]	关贝贝, 王燕娟, 陈海峰, 陈水莲, 沙爱华, 曹东. CRISPR/Cas9介导的多基因编辑系统在大豆中的应用[J]. 中国油料作物学报, 2021, 43(1): 149-.
[13]	熊艳萍, 许崇晶, 单金明, 赵琳. 利用发根农杆菌体系检测大豆CRISPR/Cas9系统的靶点[J]. 中国油料作物学报, 2021, 43(1): 161-.
[14]	包冬芳, 董海冉, 王俊, 张婵娟, 赵雪, 韩英鹏. 大豆胞囊线虫病抗性候选基因GmRSCN-6 克隆与表达分析[J]. 中国油料作物学报, 2020, 42(5): 826-.
[15]	王荣繁, 杨存义. 大豆种子耐储藏的生物学机制研究进展[J]. 中国油料作物学报, 2020, 42(5): 911-.