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中国油料作物学报 ›› 2021, Vol. 43 ›› Issue (4): 724-.doi: 10.19802/j.issn.1007-9084.2020096

• 土肥植保 • 上一篇    下一篇

基于ISSR与ITS-RFLP的花生白绢病菌遗传多样性分析

宋万朵(1988-),硕士,从事油料作物病害研究,E-mail:songwanduo@caas.cn   

  1. 中国农业科学院油料作物研究所/油料作物生物学与遗传育种重点实验室,武汉430062
  • 出版日期:2021-08-28 发布日期:2021-08-31
  • 通讯作者: 晏立英,研究员,从事油料作物病害研究,E-mail:yanliying@caas.cn; 廖伯寿,研究员,从事花生病害研究,E-mail:liaoboshou@caas.cn
  • 基金资助:
    国家重点研发计划(2018YFD0201000);国家自然科学基金(31971981);现代农业产业技术体系(CRAS-13)

Genetic diversity of Sclerotium rolfsii infecting groundnut based on ISSR and ITS-RFLP

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
  • Online:2021-08-28 Published:2021-08-31

摘要: 本研究利用简单重复序列区间(Inter Simple Sequence Repeat,ISSR)和ITS-RFLP技术,对采自我国12个省市的39个花生白绢病菌菌株进行遗传多样性分析。结果表明:从筛选出的17条ISSR引物共扩增出269条DNA谱带,其中多态性谱带266条,平均多态性百分率为98.9%,UPGMA聚类分析将这些菌株分为4个类群。限制性内切酶AluⅠ、RsaⅠ、HpaⅡ和MboⅠ作用内部转录间隔区(Internal Transcribed Spacer,ITS)PCR扩增产物产生的谱带,利用ITS-RFLP分析遗传多样性,将39个菌株划分为7个组群。ISSR和ITS-RFLP的结果均表明花生白绢病菌存在较高的遗传变异,但其遗传相似性与地理来源无明显相关。

关键词: 花生白绢病, Sclerotium rolfsii, ISSR, ITS-RFLP, 遗传多样性

Abstract: Inter simple sequence repeat (ISSR) and ITS-RFLP were used to analyze the genetic diversity of 39 Sclerotium rolfsii peanut strains collected from 12 provinces in China. A total of 269 DNA bands were amplified from 17 ISSR primers, 266 bands accounting for 98.9% showed polymorphic among them. These strains were grouped into 4 clusters by UPGMA analysis according to ISSR polymorphic bands. Variation in internal transcribed spacers (ITS) was examined following restriction enzyme digests. Restriction fragment length polymorphisms (RFLPs) were observed in ITS fragments digested by AluⅠ, RsaⅠ, HpaⅡand MboⅠ, and divided 39 strains into seven groups. The results of ISSR and ITS-RFLP showed that there was high level of genetic variation among S.rolfsii strains, and genetic groups did not correlate with geographical origin.

Key words: peanut southern stem rot, Sclerotium rolfsii, ISSR, ITS-RFLP, genetic diversity

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