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中国油料作物学报 ›› 2021, Vol. 43 ›› Issue (6): 1087-1098.doi: 10.19802/j.issn.1007-9084.2020207

• 栽培生理 • 上一篇    下一篇

不同生育期芝麻根系分泌物对连作障碍的响应及其自毒作用

吕丰娟(), 张志华, 汪瑞清, 袁展汽, 林洪鑫, 魏林根(), 肖运萍()   

  1. 江西省农业科学院土壤肥料与资源环境研究所,农业农村部长江中下游作物生理生态与耕作重点实验室,国家红壤改良工程技术研究中心,江西 南昌,330200
  • 收稿日期:2020-07-13 出版日期:2021-12-22 发布日期:2021-12-23
  • 通讯作者: 魏林根,肖运萍 E-mail:fengjuanlv@126.com;lgw0021@163.com;xyp-801@163.com;lgw0021@163.com
  • 作者简介:吕丰娟(1984- ),女,山东泰安人,助理研究员,博士,主要从事花生芝麻高产栽培技术研究,E-mail: fengjuanlv@126.com
  • 基金资助:
    国家自然科学基金(31560367);国家现代农业产业技术体系建设项目(CARS-14-2-15);江西省花生芝麻产业技术体系栽培岗位建设项目(JXARS-18)

Response of sesame root exudates at different growing stages to continuous cropping and its autotoxicity

Feng-juan LYU(), Zhi-hua ZHANG, Rui-qing WANG, Zhan-qi YUAN, Hong-xin LIN, Lin-gen WEI(), Yun-ping XIAO()   

  1. Soil and Fertilizer & Resources and Environment Institute, Jiangxi Academy of Agricultural Sciences, Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs, National Engineering and Technology Research Center for Soil Improvement, Nanchang 330200, China
  • Received:2020-07-13 Online:2021-12-22 Published:2021-12-23
  • Contact: Lin-gen WEI,Yun-ping XIAO E-mail:fengjuanlv@126.com;lgw0021@163.com;xyp-801@163.com;lgw0021@163.com

摘要:

为探讨芝麻根系分泌物中可能导致连作障碍的化感物质,以江西主栽品种金黄麻为材料,设置正茬与连作处理,研究芝麻现蕾期、盛花期和成熟期根系分泌物的组分变化与差异。结果表明:正茬芝麻根系分泌物对芝麻种子的发芽表现出“低促高抑”现象。正茬和连作芝麻在现蕾期、盛花期和成熟期的根系分泌物中鉴定出18~28种有机物。现蕾和盛花期芝麻根系分泌物均含有酯类、烃类、酸类、酚类、胺类、醇类和酮类物质,以反式-(2,3-二苯基环丙基)-苯亚砜甲酯相对丰度为最高;成熟期比现蕾和盛花期多醚类物质,以辛乙烯二醇单正十二烷基酯相对丰度为最高。酯类物质是芝麻根系分泌物的重要组分,尤其在现蕾期其总相对丰度达到75.12%(正茬)、52.96%(连作)。其中,邻苯二酸二异辛酯和辛乙烯二醇单正十二烷基酯的相对丰度在连作芝麻根系分泌物比正茬中高9.48和5.73个百分点。酸类物质在芝麻成熟期根系分泌物中含量最高,总相对丰度在正茬与连作芝麻根系分泌物中分别达17.53和42.71个百分点。其中,连作芝麻根系分泌物中棕榈酸、亚油酸和硬脂酸的相对丰度依次提高15.78、1.33和1.15个百分点;油酸、肉豆蔻酸仅在连作芝麻根系分泌物中检测到,相对丰度为10.66和1.62个百分点。与正茬相比,芥酸酰胺在现蕾期和盛花期的连作芝麻根系分泌物中相对丰度分别提高21.68和11.51个百分点;油酸酰胺相应提高2.16和7.44个百分点。综上,连作改变了芝麻根系分泌物组分。

关键词: 芝麻, 连作, 根系分泌物, 化感物质, 生育期

Abstract:

The objective of this study was to explore the potential allelochemicals in sesame root exudates that might cause sesame continuous cropping obstacles. Cultivar Jinhuangma was selected as material during budding, blooming and maturing stages under normal rotation (CK) and continuous cropping (CC). Sesame root exudates showed "low promotion and high inhibition" to seed germination. 18 to 28 organic compounds were identified in sesame root exudates at budding, blooming and maturing stages under CK and CC treatments. Sesame root exudates at budding and blooming stages contained esters, hydrocarbons, acids, phenols, amines, alcohols and ketones. Among them, the relative abundance of trans-(2,3-diphenylcyclopropyl)-methyl phenyl sulfoxide was the highest. Polyethers were found at maturing stage, but not at budding and blooming stages. The relative abundance of octaethyleneglycol monododecylether at maturing stage was the highest. Esters was important part in sesame root exudates, with the total relative abundance to 75.12% (CK) and 52.96% (CC) respectively at budding stage. Compared to CK, the relative abundance of diisooctyl phthalate and octaethyleneglycol monododecylether was 9.48 and 5.73 percentage points higher in root exudates of continuous cropping sesame. As the main source of allelochemicals, acidic substances had the highest content in root exudates at maturing stage. The total relative abundance of acids reached to 17.53% and 42.71% in exudates under CK and CC treatments respectively. Compared to CK, the relative abundances of palmitic acid, linoleic acid and stearic acid were higher by 15.78, 1.33 and 1.15 points respectively. Both oleic acid and myristic acid were detected in root exudates of continuous cropping sesame only, with the relative abundance of 10.66 and 1.62 points respectively. Compared with CK, the relative abundance of erucamide was 21.68 and 11.51 points higher at budding and blooming stages in the continuous cropping sesame root exudate, while the corresponding oleic acid amide was 2.16 and 7.44 points higher respectively. In summary, continuous cropping changed the compounds of sesame root exudates.

Key words: sesame, continuous cropping, root exudates, allelochemical, growing stage

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