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中国油料作物学报 ›› 2021, Vol. 43 ›› Issue (5): 872-.doi: 10.19802/j.issn.1007-9084.2020246

• 栽培生理 • 上一篇    下一篇

外源褪黑素对高氮下大豆氮代谢及生长发育的影响

王华美(1996- ),女,硕士研究生,主要研究方向为大豆生理生化,E-mail: whm2538505150@163.com   

  1. 1.黑龙江八一农垦大学农学院,黑龙江 大庆, 163319; 2.国家杂粮工程技术研究中心,黑龙江 大庆, 163000
  • 出版日期:2021-11-01 发布日期:2021-11-01
  • 通讯作者: 张玉先(1968- ),男,教授,博士,主要研究方向为土壤耕作和大豆高产栽培技术研究,E-mail: zyx_lxy@126.com; 于高波(1985- ),女,副教授,硕导,主要从事大豆逆境栽培生理及分子研究,E-mail: yugaobo81@163.com
  • 基金资助:
    国家重点研发项目(2018YFD1000905);国家现代农业产业技术体系项目(CARS-04-01A);黑龙江省应用技术研究与开发计划项目(GA19B101-02);黑龙江省农垦总局重点科研计划项目(HKKY190206-01);人才引进研究启动计划(XYB202011)

Effects of exogenous melatonin on nitrogen metabolism and growth of soybean under high nitrogen

  1. 1. College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. National Coarse Cereals Engineering Research Center,Daqing 163000, China
  • Online:2021-11-01 Published:2021-11-01

摘要: 过量的氮素供应会对大豆体内氮代谢及生长发育水平产生一系列的不良影响。因此,探索相应的补救措施具有十分重要的科学与实践意义。为此,本试验针对外源褪黑素对高氮环境下大豆氮代谢活动及整体生长发育的调控作用开展相关研究,分别采用7.5 mmol·L-1(对照组)和15 mmol·L-1(高氮组)的铵态氮作为唯一氮源,于大豆V3期进行100 μmol·L-1褪黑素的喷施处理。研究结果表明,外源褪黑素提高了高氮环境下大豆根系及根瘤的发育水平,并通过增强氮同化过程中谷氨酰胺合成酶等关键酶的活性,提高了氮代谢水平。此外,褪黑素还诱导了叶肉细胞内抗氧化酶活性的进一步升高,降低了膜脂过氧化程度,使光合等碳代谢能力也得到了相应的缓解。因此,褪黑素的施用有利于高氮环境下大豆碳、氮代谢平衡的恢复,进而促进各部分干物质的积累,缓解高氮对大豆生长发育的不利影响。

关键词: 褪黑素, 大豆, 高氮, 氮代谢, 生长发育

Abstract: Excessive nitrogen supply has a series of adverse effects on soybean nitrogen metabolism and growth. Thus, it is of major scientific and practical significance to explore the corresponding remedial measures. Therefore, the regulation of exogenous melatonin of soybean nitrogen metabolism and development under high nitrogen level was conducted in this study. Ammonium nitrogen of 7.5 mmol·L-1 (control group) and 15 mmol·L-1 (high nitrogen group) was used as the only nitrogen source, and 100 μmol·L-1 melatonin was sprayed at V3 stage of soybean. The results showed that exogenous melatonin was beneficial to the development of soybean roots and nodules under high nitrogen condition, and increased the level of nitrogen metabolism by enhancing the activities of key enzymes such as glutamine synthetase in the process of nitrogen assimilation. In addition, melatonin also stimulated a further increase in the activities of antioxidant enzymes in mesophyll cells, reduced the degree of membrane lipid peroxidation, and then alleviated the inhibitions of photosynthesis and other carbon metabolism level. To sum up, the application of melatonin was beneficial to the recovery of carbon and nitrogen metabolism balance of soybean under high nitrogen, and then promoted the accumulation of dry matter in various tissues, and alleviated the adverse effects of high nitrogen on the plant growth.

Key words: melatonin, soybean, high nitrogen, nitrogen metabolism, growth

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