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中国油料作物学报 ›› 2023, Vol. 45 ›› Issue (1): 30-37.doi: 10.19802/j.issn.1007-9084.2021332

• 种质资源·遗传育种 • 上一篇    下一篇

利用分子标记辅助选择选育甘蓝型油菜有限花序新品种

李开祥(), 杜德志()   

  1. 青海大学农林科学院, 国家油菜改良青海分中心,青海省春油菜遗传改良重点实验室,农业农村部春油菜科学观测实验站,青海省春油菜研究开发中心,青海省春油菜工程技术研究中心,青海 西宁,810016
  • 收稿日期:2022-01-04 出版日期:2023-02-25 发布日期:2023-03-03
  • 通讯作者: 杜德志 E-mail:18997174190@163.com;qhurape@126.com
  • 作者简介:李开祥,男,助理研究员,研究方向为春油菜遗传育种,E-mail: 18997174190@163.com
  • 基金资助:
    国家自然科学基金(31960434);现代农业产业技术体系建设专项(CARS-12)

Breeding of determinate inflorescence new varieties of Brassica napus by molecular marker-assisted selection (MAS)

Kai-xiang LI(), De-zhi DU()   

  1. Academy of Agricultural and Forestry Sciences of Qinghai University, Qinghai Research Branch of the National Rapeseed Genetic Improvement Center, Key Laboratory of Spring Rapeseed Genetic Improvement, Spring Rape Scientific Observation Experimental Station of Ministry of Agriculture and Rural Areas, Qinghai Research and Development Center for Spring Rapeseed, Qinghai Province Spring Rape Engineering Technology Research Center, Xining 810016, China
  • Received:2022-01-04 Online:2023-02-25 Published:2023-03-03
  • Contact: De-zhi DU E-mail:18997174190@163.com;qhurape@126.com

摘要:

为提高品种的抗倒性,以甘蓝型有限花序品系4769为供体亲本,分别与早熟甘蓝型油菜保持系144B和优良恢复系111R、122R、361R、536R杂交,并利用与有限花序基因Bnsdt1Bnsdt2紧密连锁的分子标记进行甘蓝型油菜有限花序性状的辅助选择育种。结果表明,共筛选到4个与Bnsdt1Bnsdt2紧密连锁的共显性分子标记(S02、Indel02、BnW09和BnW18),可用于回交转育过程中的分子标记辅助选育,最终培育出具有有限花序性状、抗倒性明显增强的保持系Y144B、不育系Y144A、优良恢复系Y111R、Y122R、Y361R、Y536R;经杂交组配和区域试验选育出一个甘蓝型油菜有限花序新品种青杂有限1号,株高适中,分枝低,抗倒伏性提高。

关键词: 甘蓝型油菜, 分子标记辅助选择, 有限花序, 抗倒伏性, 品种选育

Abstract:

To improve the lodging resistance, Brassica napus determinate inflorescence line was used as donor parent crossed with a maintainer line 144B and other 4 superior restorer lines (111R, 122R, 361R, and 536R) respectively. Molecular markers closely linked with the determinate inflorescence genes Bnsdt1 and Bnsdt2 were used to assist the selection of the breeding in early generations. Results showed that 4 co-dominant molecular markers (S02, InDel02, BnW09 and BnW18) closely linked to determinate inflorescence genes Bnsdt1 and Bnsdt2 were screened out, and could be used for marker-assisted selection during backcross. Thud the maintainer line Y144B, sterile line Y144A and 4 superior restorer lines Y111R, Y122R, Y361R and Y536R with determinate inflorescence and obviously improved lodging resistance were developed. A new B. napus determinate inflorescence variety Qingza determinate 1, was then bred by hybridization and regional test, which had medium plant height, low branching, and improved lodging resistance.

Key words: Brassica napus, molecular marker-assisted selection (MAS), determinate inflorescence, lodging resistance, variety breeding

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